Antibacterial Activity of Extra Ethanol Kopasanda Leaves (Chromolaena Odorate L.) Against Pathogenic Bacteria of Urinary Tract Infection by TLC-Bioautography and Agar Diffusion

Alif Yusra Wirawan, Rachmat Kosman, Herwin Herwin

Sari


Kopasanda  (Chromolaena odorata L.) leaves are one of the plants (herbs) containing various types of secondary metabolites, including saponins, tannins, alkaloids, and flavonoids, which function as antibacterial agents used as wound medicine for the community. This study aims to determine the antibacterial activity of the ethanol extract of Kopasanda (Chromolaena odorate L.) leaves using the TLC-bioautography and diffusion methods. The results of the antibacterial activity screening test obtained the active extract at a concentration of 0.1% against Escherichia coli and Staphylococcus aureus. The results of the antibacterial activity test using the TLC-Bioautography method with the eluent N-Hexane:Ethyl Acetate (4:1) showed results from Staphylococcus aureus bacteria with Rf values = 0.90, 0.78, 0.67, 0.52, 0.38, 0.27, 0.14, 0.05, and Escherichia coli bacteria with Rf values = 0.90, 0.52, 0.38, 0.27, 0.14, 0.05. Based on the results of testing the antibacterial activity with the agar diffusion method, the largest diameter of the inhibition zone at a concentration of 10% was obtained for Escherichia coli bacteria with an inhibition zone diameter of 13.59 mm, and Staphylococcus aureus with an inhibition zone diameter of 15.52 mm.


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Adnan ML. Wanita Usia 26 Tahun, Multigravida Hamil 35 Minggu Dengan Diagnosis Infeksi Saluran Kemih. JIMKI: Jurnal Ilmiah Mahasiswa Kedokteran Indonesia. 2019; 7(2):54–59

Yusnita R, Meylina L, Ibrahim A, Rijai L. Kajian Efektivitas Penggunaan Antibiotik Pada Pasien Infeksi Saluran Kemih (ISK) di Rumah Sakit Samarinda Medika Citra (SMC) Kota Samarinda. In: Proceeding of Mulawarman Pharmaceuticals Conferences (Proc. Mul. Pharm. Conf.). 2017, pp. 205–222

Benjamin VT, Oguntimelun I. Phytochemical and Antibacterial Studies on the Essential Oil of Euphatorium Odoratum. Department of Pharmacognosy, School of Pharmacy, University of Lagos, Nigeria.

Rusli R, Ramadhan AM. Aktivitas Antibakteri Daun Kirinyuh (Chromolaena Odorata (L.) RM King & H. Rob.) Terhadap Bakteri Gangren. In: Proceeding of Mulawarman Pharmaceuticals Conferences. 2015, pp. 75–81

Andika B, Halimatussakdiah H, Amna U. Analisis Kualitatif Senyawa Metabolit Sekunder Ekstrak Daun Gulma Siam (Chromolaena odorata L.) Di Kota Langsa, Aceh. QUIMICA: Jurnal Kimia Sains dan Terapan. 2020; 2(2):1–6

Rusli RK, Melinda P. Penelusuran Fungsi Endofit Pada Daun Kopasanda (Chromolaena odorata L.) yang Berpotensi Sebagai Penghasil Antibakteri Terhadap Bateri Penyebab Infeksi Kulit. As-Syifaa Jurnal Farmasi. 2020; 12(1):64–69

Aslah A, Lolo WA, Jayanto I. Aktivitas Antibakteri Dan Analisis Klt-Bioautografi dari Fraksi Daun Mengkudu (Morinda citrifolia L.). Pharmacon. 2019; 8(2):505

Hibai YRA, Herwin, Kosman R. Antibacterial Activity Assay of Ethanolic Extract of Bulbs Sticky Taro (Colocacia esculenta) Use TLC-Bioautografi. Jurnal As-Syifaa. 2015; 07(01):76–84

Deponda RA, Fitriana F, Nuryanti S, Herwin H. Isolasi Fungi Endofit Kulit Buah Merah (Pandanus conoideus Lam.) yang Berpotensi Sebagai Antibakteri Secara Metode KLT - Bioautografi. As-Syifaa Jurnal Farmasi. 2019; 11(2):147–153

Herwin H, Nuryanti S. Skrining Aktivitas Antimikroba Ekstrak Herba Belimbing Tanah (Oxalis corniculata L.) secara KLT-Bioautografi dan Difusi Agar. As-Syifaa Jurnal Farmasi. 2012; 4(1):74–81

Fitrah M. Identifikasi Ekstrak Daun Kopasanda (Chromolaena odorata Linn) Terhadap Sel Antiproliferasi Tikus Leukemia L1210. Jurnal Farmasi UIN Alauddin Makassar. 2016; 4(3):99–105

Manik DF, Hertiani T, Anshory H. Analisis Korelasi Antara Kadar Flavonoid dengan Aktivitas Antibakteri Ekstrak Etanol dan Fraksi-Fraksi Daun Kersen (Muntingia Calabura L.) terhadap Staphylococcus aureus. Khazanah. 2014; 6(2):1–11

KC AP, Kumar K, Vijayan M. A Cross Sectional Study on Distribution of Urinary Tract Infection and Their Antibiotic Utilisation Pattern in Kerala. International Journal of Research in Pharmaceutical and Biomedical Sciences. 2012; 3:1125–1130

Alkhyat SH, Al-Maqtari MA. Prevalence of Microorganisms Isolates from Urinary Tract Infections at Some Hospitals in Sana’a City, Yemen. Int. J. Curr. Microbiol. Appl. Sci. 2014; 3(6):876–885

Jarvis TR, Chan L, Gottlieb T. Assessment And Management Of Lower Urinary Tract Infection In Adults. Aust Prescr. 2014; 37(1):7–9

Kibret M, Abera B. Prevalence And Antibiogram Of Bacterial Isolates From Urinary Tract Infections At Dessie Health Research Laboratory, Ethiopia. Asian Pac J Trop Biomed. 2014; 4(2):164–168

Swetha VV, Rao US, Prakash PH, Subbarayudu S. Aerobic Bacteriological Profile Of Urinary Tract Infections In A Tertiary Care Hospital. Int. J. Curr. Microbiol. Appl. Sci. 2014; 3(3):120–125

Seta IS, Indah HL, Rizka R. Pola Kepekaan Bakteri Penyebab Infeksi Saluran Kemih pada Anak Terhadap Antimikroba. Majalah Kedokteran Sriwijaya. 2015; 47(2):85–90

Wendersteyt NV, Wewengkang DS, Abdullah SS. Uji Aktivitas Antimikroba Dari Ekstrak Dan Fraksi Ascidian herdmania momus Dari Perairan Pulau Bangka Likupang Terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium Dan Candida albicans. PHARMACON. 2021; 10(1):706–712

Wijayati N, Astutiningsih C, Mulyati S. Transformasi α-Pinena Dengan Bakteri Pseudomonas aeruginosa ATCC 25923. Biosaintifika: Journal of Biology & Biology Education. 2014; 6(1):24–28

Sari R, Apridamayanti P, Pratiwi L. Efektivitas SNEDDS Kombinasi Fraksi Etil Asetat Daun Cengkodok (Melasthoma malabathricum)-Antibiotik Terhadap Bakteri Hasil Isolat Dari Pasien Ulkus Diabetik. Pharmaceutical Journal of Indonesia. 2022; 7(2):105–114

Hoffmann JJ, Timmermann BN, Mclaughlin SP, Punnapayak H. Potential Antimicrobial Activity of Plants from the Southwestern United States. International Journal of Pharmacognosy. 2008; 31(2):101–115

Romsiah, Utami DP. Identifikasi Sakarin dan Siklamat pada Minuman Es Tidak Bermerk yang Dijual Di Pasar 16 Ilir Palembang Dengan Menggunakan Metode Kromatografi Lapis Tipis. Jurnal Ilmiah Bakti Farmasi. 2018; 3(1):47–52

Samosir AS, Bialangi N, Iyabu H. Analisis Kandungan Rhodamin B pada Saos Tomat yang Beredar Dipasar Sentral Kota Gorontalo Dengan Menggunakan Metode Kromatografi Lapis Tipis (KLT). Jurnal Entropi. 2018; 13(1):45–49




DOI: https://doi.org/10.56711/jms.v3i2.979

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