Antibacterial Activity of Keji Beling (Strobilanthes crispa) Ethanol Extract Using TLC – Bioautography

Nurul Fadhillah Junardin, Siska Nuryanti, Ira Asmaliani

Sari


Antibacterials are compounds that inhibit the growth of bacteria that are harm humans. Keji Beling (Strobilanthes crispa) contains secondary metabolites such as saponins, flavonoids, terpenoids, polyphenols, and potassium. One part used in traditional medicine is the leaves which can treat various diseases. In this study, the ethanol extract of Keji Beling leaves was tested using TLC-bioautography This study tested the ethanol extract of Keji Beling leaves using TLC-bioautography. The examination begins with a screening for antibacterial activity against several pathogen bacteria, with results that inhibit the growth of Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermis, Pseudomonas aeruginosa, Streptococcus mutans, Salmonella typhii, Shygella dysenteriae, and Vibrio cholera. Test results using the TLC-bioautography method using n-hexane eluent: ethyl acetate (1: 2) found that the spots were active against bacteria Pseudomonas aeruginosa and Staphylococcus aureus Rf value 0.74 and bacteria Bacillus subtilis, Escherichia coli, Salmonella thypii, Staphylococcus epidermidis, Staphylococcus mutans, Shygella dysenteriae, Vibrio cholera with an Rf value of 0.83 The results of the identification of chemical components showed the presence of positive compounds belonging to the class of flavonoids and anthraquinones. The ethanol extract of keji beling leaves contains a class of flavonoid compounds that have the potential as antibacterial agents with an Rf value of 0.83


Teks Lengkap:

PDF

Referensi


Jawetz, E., Melnick, J. L., & Adelberg, E. A. Mikrobiologi kedokteran edisi XXII. Jakarta: Salemba Medika.2001.

Asnita, Rachmat K, Herwin, dan Ayyub H. N. 2020. Isolasi dan Identifikasi Fungi Endofit Batang Seseru (Euphorbia antiquorum L.) sebagai Penghasil Antibakteri dengan Metode KLT-Bioautografi. As-Syifaa Jurnal Farmasi.2020; 12(2):144 – 149.

Pratiwi RH. Mekanisme Pertahanan Bakteri Patogen Terhadap Antibiotik. J Pro-Life. 2017;4(3):418–429

Amalia SN, Syafnir L, Purwanti L. Pengaruh letak daun terhadap kadar katekin total pada daun kejibeling (Strobilanthes Crispus). [Karya ilmiah]. Bandung: Universitas Islam Bandung.2015.

Adibi S, Nordan H, Ningsih SN, Kurnia M, Rohiat S. Aktivitas antioksidan dan antibakteri ekstrak daun Strobilanthes crispus Bl ( Keji Beling ) terhadap Staphylococcus aureus Dan Escherichia coli. ALOTROP J Pendidik dan Ilmu Kim. 2017;1(2):148–154.

Rivai H, Yulianti S, Chandra B. Analisis Kualitatif dan Kuantitatif dari Ekstrak Heksan, Aseton, Etanol, dan Air dari Daun Salam (Syzygium polyanthum (WIGHT) Walp.). J Fak Farm Univ Andalas. 2019;(March):1–13.

Mardiana RN, Handayani N. Antibacterial activity of the sambiloto leaf extracts (Andrographis paniculata) to Bacillus cereus and Pseudomonas aeruginosa. Asian Journal of Natural Product Biochemistry. 2016;14(1):19-24.

Risdayanti, R., Nuryanti, S., & Herwin, H. (2020). ‘Aktivitas Antibakteri Ekstrak Etanol Daun Patikan Kebo (Euphorbia hirta L.). 2020.2.23–29.

Fitriana, F., Abdullah, A. A., & Achmar, A. A. Profil Bioautogram Ekstrak Fermentat Isolat Fungi Endofit Dari Daun Galing-Galing (Cayratia trifolia L) Sebagai Antibakteri’. Jurnal Ilmiah As-Syifaa. 2019. 11(1). 17–23.

Deponda, R. A., Fitriana, Siska N., dan Herwin. Isolasi Fungi Endofit Kulit Buah Merah (Pandanus conoideus Lam.) yang Berpotensi sebagai Antibakteri Secara Metode KLT-Bioautografi’. As-Syifaa Jurnal Farmasi.2019. 11(2). 147-153.

Marpaung, M. P., & Wahyuni, R. C. Identifikasi Dan Penetapan Kadar Flavonoid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers). Talenta Conference Series: Tropical Medicine (TM). 2018. 1(3). 095–098

Kristanti, A. N., Aminah, N. S., Tanjung , M., Kurniadi, B. 2008, Buku Ajar Fitokimia, Airlangga University Press, Surabaya.

Banu, R. H., Nagarajan, N. TLC and HPTLC fingerprinting of leaf extracts of Wedelia chinensis (Osbeck) Merrill. Journal of Pharmacognosy and Phytochemistry. 2014. 2(6). 29-33.

Paputungan, W. A., Lolo, W. A., & Siampa, J. P. Aktivitas Antibakteri Dan Analisis KLT-Bioautografi Dari Fraksi Biji Kopi Robusta (Coffea canephora Pierre ex A. Froehner). Pharmacon. 2019. 8(3). 516.

Aslah, Aprilia Pratiwi, Widya Astuty Lolo, dan Imam Jayanto. 2019. “Aktivitas Antibakteri Dan Analisis KLT-Bioautografi Dari Fraksi DAUN Mengkudu (Morinda citrifolia L.) Program Studi Farmasi FMIPA UNSRAT Manado , 95115

Sapara, T. U., Waworuntu, O. dan Juliatri. (2016). Efektivitas antibakteri ekstrak daun pacar air (Impatiens balsamina L.) terhadap pertumbuhan Porphyromonas gingivalis. Pharmacon, Jurnal Ilmiah Farmasi, 5(4): 10-17.




DOI: https://doi.org/10.56711/jms.v4i1.1037

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

Indexed by:

     


ISSN: 2808-392X | e-ISSN: 2808-3911

Editor's Address:

3rd Floor Laboratory Faculty of Pharmacy,
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

Phone: +62 821 89555447
Fax: +62411425619
E-mail: jmic.sci@umi.ac.id