UJI AKTIVITAS ANTIOKSIDAN FRAKSI n-HEKSAN, ETIL ASETAT, DAN AIR EKSTRAK DAUN BIWA (Eriobotrya japonica (Thunb.) Lindl.) DENGAN METODE DPPH

Kristi Melsi, Vivin Nopiyanti, Endang Sri Rejeki

Abstract


Loquat leaf (Eriobotrya japonica (Thunb.) Lindl.)  have been known to contain flavonoid compounds, alkaloids, tannins, saponins, triterpenoids and glycosides, these compounds have the potential to be antioxidants and can ward off free radicals. The purpose of this study was to determine the fraction of n-hexane ethyl acetate and water with extract of loquat leaf which have antioxidant activity, and to find out, respectively, which fraction had the most active activity from the IC50 value in activity antioxidant. The extraction method of loquat was macerated using 96% ethanol and then fractionated with n-hexane, ethyl acetate, and water. The obtained fraction was tested for antioxidant activity against free radicals using the DPPH method using UV-Vis spectrophotometer at the wavelength of 516 nm. The results of the loquat leaf fraction test have antioxidant activity which can be seen from the IC50 value, namely the n-hexane fraction is 1792 ppm, the ethyl acetate fraction is 89.60 ppm, the water fraction is 394.99 ppm, and quercetin is 13.03 ppm, so the most active antioxidant is obtained ethyl acetate


Keywords


Antioxidant; DPPH; Eriobotrya japonica (Thunb.) Lindl.; Loquat leaf.

References


Bangun E. Biwa Tanaman Buah Langka, Kebun Percobaan Tanaman Buah Berastagi. Berastagi; 2004.

Yokota, J, Takuma D, Hamada A, Onogawa M, Yoshioka S., Kusunose M. et al. Gastroprotective activity of Eriobotrya japonica seed extract on experimentally induced gastric lesions in rats. J Nat Med. 2008;62(1):96–100.

Kumaresan S, Karthi V, Senthilkumar V, Balakumar BS, Stephen A. Biochemical Constituents and Antioxidant Potential of Endophytic Fungi isolated from the Leaves of Azadirachta indica A. Juss (Neem) from Chennai, India. J Acad Ind Res. 2015;3(8):355.

Molyneux P. The use of the stable free radical diphenylpicry hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol. 2004;26(2):211–9.

Ditjen POM DR. Cara Pembuatan Simplisia. Departemen kesehatan RI. Jakarta; 1985.

Ditjen POM DR. Parameter Standar Umum Ekstrak Tumbuhan Obat. Departemen Kesehatan Republik Indonesia. Jakarta; 2000.

Hamsidi R, Widyawaruyanti A, Hafid AF, Ekasari W, Kasmawati H, Akib NI, et al. In vitro antimalarial activity of chloroform, n-butanol, and ethyl acetate fractions of ethanol extracts of Carthamus tinctorius Linn. Flowers. Asian J Pharm Clin Res. 2018;11(2):121–3.

I Kuncahyo S. Uji aktivitas antioksidan ekstrak belimbing wuluh (Averrhoa blimbi L.) terhadap DPPH. Semin Nas Teknol. 2007;1–1.

Marianne, Septiani R, Yuliana Y. Aktivitas Antioksidan Ekstrak Etanol Daun Biwa (Eriobotrya japonica (Thunb.) Lindl.) Terhadap DPPH (1,1-diphenyl-2-picrylhydrazyl). Talenta Conference Series: Tropical Medicine (TM). 2018. p. 086–9.

Mukhriani. Ekstraksi, Pemisahan Senyawa, dan Identifikasi Senyawa Aktif. J Kesehat. 2014;7(2):363.

Kuncahyono I and Sunardi. Uji Aktivitas Antiokisdan Ekstrak Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). Seminar Nasional Teknologi. 2007:1-9




DOI: https://doi.org/10.56711/jifa.v14i2.851

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ISSN: 2085-4714 | e-ISSN: 2502-9444

Editor's Address:

Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

E-mail: jurnal.farmasi@umi.ac.id

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