IDENTIFIKASI KUALITATIF FENOLIK DAN PENAPISAN AKTIVITAS PEREDAMAN RADIKAL DPPH MENGGUNAKAN KLT PADA EKSTRAK BATANG Pluchea indica L.

Ni Putu Ermi Hikmawanti, Agustin Yumita, Jihan Esa Siregar

Abstract


Pluchea indica L. (beluntas) from the Asteraceae is a plant known to be rich in phenolics. Studies on the phenolic content of P. indica stems have not been explored much. The purpose of this study is to qualitatively identify the presence of phenolic compounds and to screen for the radical scavenging ability of DPPH in P. indica stem extracts using the TLC method. Extraction was carried out with the ultrasonic directly and sequentially using 50% ethanol as solvent. Separation was carried out on a silica gel plate GF254 with ethyl acetate: water: formic acid: toluene (20:2:2:1) as the mobile phase. Gallic acid and chlorogenic acid were used as comparisons. Identification of the presence of phenolic using FeCl3 5% as a spray reagent, while screening for radical scavenging activity using DPPH 0.1 mM as a spray reagent. The results showed that the 50% ethanol extracts of P. indica stems, both obtained from direct and stratified extraction, indicated the presence of phenolic compounds as antioxidants. The phenolic compounds detected were identical to gallic acid and chlorogenic acid, respectively, at hRf values of 89.4 and 55.3. The development of phenolic utilization from P. indica stems as a source of antioxidants still needs to be studied.

Keywords


Antioksidan; Beluntas; DPPH; Fenolik; KLT; Pluchea indica.

References


Direktorat Obat Asli Indonesia. Taksonomi: Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Jakarta: Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI). 2008

Alvin A, Miller KI, Neilan BA. Exploring the Potential of Endophytes from Medicinal Plants as Sources of Antimycobacterial Compounds. Microbiol Res. 2014; 169:483–495

Roosita K, Kusharto CM, Sekiyama M, Fachrurozi Y, Ohtsuka R. Medicinal Plants Used by the Villagers of a Sundanese Community in West Java, Indonesia. J Ethnopharmacol. 2008; 115(1):72–81

Zumsteg IS, Weckerle CS. Bakera, a Herbal Steam Bath for Postnatal Care in Minahasa (Indonesia): Documentation of the Plants Used and Assessment of the Method. J Ethnopharmacol. 2007; 111(3):641–650

Yuniarti T. Ensiklopedia Tanaman Obat Tradisional. Yogyakarta: MedPress. 2008

Andarwulan N, Kurniasih D, Apriady RA Rahmat H, Roto AV, Bolling BW. Polyphenols, Carotenoids, and Ascorbic Acid in Underutilized Medicinal Vegetables. J Funct Foods. 2012; 4:339–347

Normala H, Suhaimi MI. Quantification of Total Phenolics in Different Parts of Pluchea indica (Less) Ethanolic and Water Extracts. Pertanika J Sci Technol. 2011; 19(1):19–24

Kongkiatpaiboon S, Chewchinda S, Vongsak B. Optimization of Extraction Method and HPLC Analysis of Six Caffeoylquinic Acids in Pluchea indica Leaves from Different Provenances in Thailand. Revista Brasileira de Farmacognosia 2018; 28(2):145–150

Chewchida S, Vongsak B. Simultaneous HPTLC Quantification of Three Caffeoylquinic Acids in Pluchea indica Leaves and Their Commercial Products in Thailand. Rev Bras Farmacogn. 2019; 29:177–181

Vongsak B, Kongkiatpaiboon S, Jaisamut S, Konsap K. Comparison of Active Constituents, Antioxidant Capacity, and α-Glucosidase Inhibition in Pluchea indica Leaf Extracts at Different Maturity Stages. Food Bioscience 2018; 25:68–73

Marković S, Tošović J. Comparative Study of the Antioxidative Activities of Caffeoylquinic and Caffeic Acids. Food Chem. 2016; 210:585–592

Liu W, Li J, Zhang X, Zu Y, Yang Y, Liu W, Xu Z, Gao H, Sun X, Jiang X, Zhao Q. Current Advances in Naturally Occurring Caffeoylquinic Acids: Structure, Bioactivity, and Synthesis. J Agric Food Chem. 2020; 68(39):10489–10516

Meinhart AD et al. Study of New Sources of Six Chlorogenic Acids and Caffeic Acid. J Food Compos Anal. 2019; 82(01):103244

Dai J, Mumper RJ. Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties. Molecules. 2010; 15:7313–7352

Rafi M, Heryanto R, Septiningsih DA (eds). Kromatografi Lapis Tpis Tumbuhan Obat Indonesia. Vol. 1. Bogor: Penerbit IPB Press. 2017

Irianti T, Murti YB, Kanistri DN, Pratiwi DR, Kuswandi, Kusumaningtyas RA. DPPH Radical Scavenging Activity of Aqueous Fraction from Ethanolic Extract of Talok Fruit (Muntingia calabura L.). Tradit Med J. 2016; 21(1):38–47

Kementerian Kesehatan RI. Farmakope Herbal Indonesia (FHI). 2nd ed. Jakarta: Kementerian Kesehatan RI. 2017

Shaikh JR, Patil M. Qualitative Tests for Preliminary Phytochemical Screening: An Overview. Int J Chem Stud. 2020; 8(2):603–608

Zhang Q-W, Lin L-G, Ye W-C. Techniques for Extraction and Isolation of Natural Products: A Comprehensive Review. Chin Med.; 13(20). DOI: 10.1186/s13020-018-0177-x

Tiwari P, Kaur M, Kaur H. Phytochemical Screening and Extraction: A Review. Int Pharm Sci. 2011; 1(1):98–106

Fatmawati S, Sjahid LR, Utami NM, Kartini K. Total Phenolic, Total Flavonoid Content and in Vitro Sun Protection Factor Test of Arabica coffee Leaves Extract (Coffea arabica L). J Sci Technol Res Pharm. 2021; 1(2):57–66

Khoddami A, Wilkes MA, Roberts TH. Techniques for Analysis of Plant Phenolic Compounds. Molecules. 2013; 18(2):2328–2375

Sjahid LR, Aqshari A, Sediarso S. Penetapan Kadar Fenolik Dan Flavonoid Hasil Ultrasonic Assisted Extraction Daun Binahong (Anredera cordifolia [Ten] Steenis). J Ris Kim. 2020; 11(1):16–23

Lu H, Tian Z, Cui Y, Liu Z, Ma X. Chlorogenic Acid: A Comprehensive Review of the Dietary Sources, Processing Effects, Bioavailability, Beneficial Properties, Mechanisms of Action, and Future Directions. Compr Rev Food Sci Food Saf. 2020; :1–29

Upadhyay R, Mohan Rao LJ. An Outlook on Chlorogenic Acids-Occurrence, Chemistry, Technology, and Biological Activities. Crit Rev Food Sci Nutr. 2013; 53(9):968–984

Gulcin İ. Antioxidants and Antioxidant Methods: An Updated Overview. Arch Toxicol. 2020; 94(3):651–715

Prastiwi R, Elya B, Sauriasari R, Hanafi M and Desmiaty Y. Arginase Inhibitory, Antioxidant Activity and Pharmacognosy Study of Sterculia Macrophylla Vent. Leaves. Pharmacogn J. 2018; 10(6):1109–1113

Munteanu IG, Apetrei C. Analytical Methods Used in Determining Antioxidant Activity: A Review. Int J Mol Sci.; 22(7). DOI: 10.3390/ijms22073380




DOI: https://doi.org/10.56711/jifa.v14i2.812

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Faculty of Pharmacy, Univeristas Muslim Indonesia
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