ANALISIS KADAR FENOLIK FRAKSI ETIL ASETAT DAUN PETAI CINA (Leucaena leucocephala) (Lam.) de Wit) SECARA SPEKTROFOTOMETRI Uv-Vis

Intan Purnama Sari, Zainal Abidin, St Maryam

Abstract


Traditionally, river tamarind leaves (Leucaena leucocephala (Lam.) De Wit.) can be used as medicine for injuries, swollen, diarrhea and also as antiseptic. The river tamarind leaves contains several main compounds such as alkaloids, saponins, flavonoids, phenols, mimosine, lectin, protein, oil, calcium, phosphorus, iron, vitamin A and vitamin B. The research aimed to determine the phenolic levels found in the ethyl acetate fraction of the leaves. The extraction was done by maceration method using ethanol 96% then fractionated with ethyl acetate solvent. The obtained fraction was analysed by UV-Vis spectrophotometry method at the maximum wavelength of 761 nm using gallic acid as a standard. Based on the qualitative test, river tamarind leaves positively contained phenolic. The phenolic compound contained in the ethyl acetate fraction was 0.176 mgGAE/g in that every gram of ethyl acetate fraction had phenolic of 0.176 mg equivalent to garlic acid.


Keywords


Phenolic; Ethyl Acetate Fraction; River Tamarind Leaves (Leucaena leucocephala (Lam.) De Wit.), UV-Vis spectrophotometry.

References


Hendra R, Ahmad S, Sukari A, Shukor MY, Oskoueian E. Flavonoid analyses and antimicrobial activity of various parts of Phaleria macrocarpa (Scheff.) Boerl fruit. Int J Mol Sci. 2011; 12(6):3422-31.

Srivastava N, Chauhan AS, Sharma B. Isolation and Characterization of Some Phytochemicals from Indian Traditional Plants. Biotechnol Res Int. 2012.

Harborne JB. Phytochemical Methods A Guide To Modern Tecniques Of Plant Analysis, Third Edition. Chapman Hall. 1998.

Gustandy M, Soegihardjo CJ. Uji Aktivitas Antioksidan Menggunakan Radikal 1,1-Difenil-2- Pikrilhidrazil dan Penetapan Kandungan Fenolik Total Fraksi Etil Asetat Ekstrak Etanol Buah Anggur Bali (Vitis vinifera L.). J Farm Sains dan Komunitas. 2013;10(2):109-120.

Ritna A, Anam S, Khumaidi A. Identifikasi Senyawa Flavonoid Pada Fraksi Etil Asetat Benalu Batu (Begonia Sp.) Asal Kabupaten Morowali Utara. J Farm Galen (Galenika J Pharmacy). 2016;2(2):83-89.

Sovia S, Andayani NGPS, Prawishanti IGAR, Ermawan R, Pintaningrum Y. Acute lower limb ischaemia in patient with definite infective endocarditis. Eur Hear Journal, Suppl. 2017.

Ahmad AR, Juwita J, Ratulangi SAD. Penetapan Kadar Fenolik dan Flavonoid Total Ekstrak Metanol Buah dan Daun Patikala (Etlingera elatior (Jack) R.M.SM). Pharm Sci Res. 2015; 2(1):1-10.

Lestari ARA, Syahfitri SA, Cahyo ST, Wardaniati I, Herli MA. Aktivitas Antibakteri Seduhan Biji Pepaya (Carica papaya L) Terhadap Escherichia coli, Salmonella thypi dan Staphlycocus aureus. Journal Pharm Sci. 2018;1(2):40-45.

Stanković M. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujev J Sci. 2011; 33:63-72.

Alfian R, Susanti H. Penetapan Kadar Fenolik Total Ekstrak Metanol Kelopak Bunga Rosella Merah (Hibiscus sabdariffa Linn) dengan Variasi Tempat Tumbuh Secara Spektrofotometri. Pharmaciana. 2012;2(1):73 – 80.

Apsari PA, Sari DNE, Kusuma AP, Indrati O. Effervescent Tablet Formulation Melinjo Seed Extract (Gnetum gnemon L.) Using PEG 6000 As Lubricant and Citric Acid - Tartaric Acids As Acid Sources. J EKSAKTA. 2018;18(1):30-41.

Liu GL, Guo HH, Sun YM. Optimization of the extraction of anthocyanins from the fruit skin of rhodomyrtus tomentosa (Ait.) hassk and identification of anthocyanins in the extract using high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS). Int J Mol Sci. 2012; 13(5):6292-302

Purbowati ISM, Syamsu K, Warsiki E, Sri H. Stabilitas Senyawa Fenolik Dalam Ekstrak Dan Nanokapsul Kelopak Bunga Rosella Pada Berbagai Variasi pH, Suhu dan Waktu. Agrointek. 2016;10(1):31-40.

Dai J, Mumper RJ. Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010.

Salamah E, Ayuningrat E, Purwaningsih S. Penapisan awal komponen bioaktif dari kijing taiwan (Anodonta woodiana Lea.) Sebagai Senyawa Antioksidan. Bul Teknol Has Perikan. 2008;11(2):119-133.

Fuentes E, Báez ME, Bravo M, Cid C, Labra F. Determination of Total Phenolic Content in Olive Oil Samples by UV-visible Spectrometry and Multivariate Calibration. Food Anal Methods. 2012; 5(6):1311-1319.




DOI: https://doi.org/10.56711/jifa.v12i2.681

Refbacks

  • There are currently no refbacks.


Indexed by:

     

ISSN: 2085-4714 | e-ISSN: 2502-9444

Editor's Address:

Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

E-mail: jurnal.farmasi@umi.ac.id

View My Stats