Aktivitas Antibakteri Fraksi-Fraksi Daun Colocacia esculenta L. Terhadap Salmonella thypi Dan Staphylococcus aureus Secara Bioautography-TLC

Muzakkir Baits, Herwin herwin, ririn ririn

Sari


Antibacterial activity test has been done on Colocacia esculenta L leaves fractions. The aim of the research was to determine the antibacterial activity of Colocacia esculenta L leaves fractions delivered from Pare-Pare District of South Sulawesi on Salmonella thypii and Staphyllococcus aureus by means of vacuum liquid chromatography and Bioautographic-TLC. Results of fractionations of the ethanol extract by the former method using eluent of 100 mL n-hexane, n-hexane:ethyl acetate (10:1; 1:1), 100 mL ethyl acetate, ethyl acetate:methanol (10:1; 1:1) and 100 mL methanol were obtained 7 fractions. Bioautographic-TLC assay then carried out on the obtained fractions they were fraction 3,4,5,6 and 7 respectively and obtained Rf value 0.80 was possessed antibacterial activity on both tested bacteria.

Keywords: Leaves fraction of Colocacia esculenta L, Bioautography-TLC, Antibacterial


Teks Lengkap:

Fulltext PDF

Refbacks

  • Saat ini tidak ada refbacks.