Antibacterial Activity of N-Hexan and Ethyl Acetate Fractions of Gaharu (Aquilaria Malaccensis) Leaves against Bacteria that Cause Infection
DOI:
https://doi.org/10.56711/jms.v4i2.1139Abstract
Gaharu (Aquilaria malaccensis) leaves contain alkaloids, terpenoids, flavonoids, saponins, and tannins that can help cure infections. This study aims to determine the antibacterial activity of n-hexane and ethyl acetate fractions of Gaharu leaves against skin infection bacteria such as Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis by Bioautography-TLC. Gaharu leaves symplisia was extracted using the maceration method using ethanol 96% solvent and fractionation with solid-liquid method to obtain n-hexane and ethyl acetate fractions. The result of the screening antibacterial activity of n-hexane and ethyl acetate fractions of Gaharu leaves with concentrations of 0,5% active to 4 bacteria test. The results of antibacterial activity n-hexane and acetaet ethyl fraction by Bioautography-TLC using n-hexane:ethyl acetate (1:4) eluent showed Rf 0.67 and Rf 0,16 value in the n-hexane fraction and an Rf 0.78 value in the ethyl acetate fraction active to 4 bacteria test. The identification results of the chemical compound of the n-hexane fraction contain flavonoids and tannins (AlCl3 and FeCl3 reagents.), the ethyl acetate fraction contains flavonoids ( AlCl3 reagents).
References
Pratiwi, R.H., 2017. Mekanisme Pertahanan Bakteri Patogen Terhadap Antibiotik. Jurnal Pro-Life, 4(3), Pp.418-429.
Tari, M. And Nely, N., 2016. Uji Aktivitas Antibakteri Terhadap Beberapa Fraksi Daun Sembung Rambat (Mikania Micrantha Kunth.) Terhadap Bakteri Penyebab Penyakit Kulit. Jurnal Ilmiah Bakti Farmasi, 1(2).
Kementrian Kesehatan Republik Indonesia. (2011). Profil Kesehatan Indonesia 2010. Jakarta : Kementrian Kesehatan Republik Indonesia.
Fitriani, I.R., Fitriana, F. And Nuryanti, S., 2023. Aktivitas Antibakteri Ekstrak Etanol Daun Ketepeng Cina (Cassia Alata L.) Terhadap Beberapa Bakteri Penyebab Infeksi Kulit. Makassar Natural Product Journal (Mnpj), 1(1), Pp.22-28.
Puteri, T. And Milanda, T., 2016. Uji Daya Hambat Ekstrak Daun Lidah Buaya (Aloe Vera L.) Terhadap Bakteri Escherichia Coli dan Staphylococcus Aureus. Farmaka, 14(2), Pp.9-17.
Oroh, S.B., Kandou, F.E., Pelealu, J. And Pandiangan, D., 2015. Uji Daya Hambat Ekstrak Metanol Selaginella Delicatula dan Diplazium Dilatatum Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli. Jurnal Ilmiah Sains, Pp.52-58.
Wijayanti, E.D., 2018. Perbandingan Kadar Fenolik Total Antara Seduhan Daun Gaharu Dan Kombucha Daun Gaharu (Aquailaria Malaccensis). Jc-T (Journal Cis-Trans): Jurnal Kimia Dan Terapannya, 2(1).
Misrahanum, M., Zahira, C.A.D. And Saidi, N., 2022. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Gaharu (Aquilaria Malaccensis Lamk.) Dan Identifikasi Senyawa Dengan Metode Gc-Ms. Jurnal Pharmascience, 9(2), Pp.310-318.
Sari, R., Muhani, M. And Fajriaty, I., 2017. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Gaharu (Aquilaria Microcarpa Baill.) Terhadap Bakteri Staphylococcus Aureus dan Proteus Mirabilis. Pharmaceutical Sciences and Research, 4(3), P.4.
Suhardiman, A., Hikmiah, H. And Budiana, W., 2020. Aktivitas Fraksi Daun Gaharu (Aquilaria Malaccensis Lam) Sebagai Antijerawat dan Uji Bioautografi. Jurnal Sains Dan Teknologi Farmasi Indonesia, 9(1), Pp.1-16.
Elfasyari, T.Y., Kintoko, K. And Nurkhasanah, N., 2018, December. Gambaran Penyembuhan Luka Tikus Diabetes dengan Fraksi Etil Asetat Daun Binahong (Anredera Cordifolia (Tenore) Steenis). In Talenta Conference Series: Tropical Medicine (Tm) (Vol. 1, No. 3, Pp. 158-161).
Asnita, A., Herwin, H., Kosman, R., & Nurung, A. H. 2020. Isolasi dan Identifikasi Fungi Endofit Batang Sesuru (Euphorbia Antiquorum L.) Sebagai Penghasil Antibakteri Dengan Metode Klt-Bioautografi. Jurnal Ilmiah As-Syifaa, 12(2), 144-149.
Hibai, A.R.Y., Herwin, H. And Kosman, R., 2015. Antibacterial Activity Assay Of Ethanolic Extract Of Bulbs Sticky Taro (Colocasia Esculenta) Use Tlc-Bioautografi. As-Syifaa Jurnal Farmasi, 7(1), Pp.76-84.
Hasma, H. and Winda, W., 2019. Identifikasi Senyawa Metabolit Sekunder Ekstrak Etanol Kulit Buah Pisang Kepok (Musa paradisiaca L) dengan Metode KLT. Jurnal Kesehatan Manarang, 5(2).
Razak, A.R., Ridhay, A., Sumarni, N.K. And Rahim, E.A., 2022. Aktivitas Antibakteri Ekstrak Kulit Batang Tumbuhan Johar (Senna Siamea Lam) Pada Berbagai Polaritas Pelarut. Kovalen: Jurnal Riset Kimia, 8(2), Pp.184-195.
Latif, S.K.H., Naid, T. and Fitriana, F., 2023. Aktivitas Antibakteri Fraksinasi Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius) Terhadap Bakteri Escherichia coli Menggunakan Metode KLT-Bioautografi. Makassar Pharmaceutical Science Journal (MPSJ), pp.195-203.
Fitriani, I.R., Fitriana, F. And Nuryanti, S., 2023. Aktivitas Antibakteri Ekstrak Etanol Daun Ketepeng Cina (Cassia Alata L.) Terhadap Beberapa Bakteri Penyebab Infeksi Kulit. Makassar Natural Product Journal (Mnpj), 1(1), Pp.22-28.
Makatambah, V., Fatimawali, F. and Rundengan, G., 2020. Analisis senyawa tannin dan aktifitas antibakteri fraksi buah sirih (piper betle l) terhadap streptococcus mutans. Jurnal MIPA, 9(2), pp.75-89.
Paputungan, W.A., Lolo, W.A. And Siampa, J.P., 2019. Aktivitas Antibakteri Dan Analisis Klt-Bioautografi Dari Fraksi Biji Kopi Robusta (Coffea Canephora Pierre Ex A. Froehner). Pharmacon, 8(3), Pp.516-524.
Rusli, R., Kosman, R., Muthmainnah, M. And Nurung, A.H., 2023. Aktivitas Antibakteri Fermentat Fungi Endofit Daun Kasumba Turate (Carthamus Tinctorius L.) Asal Galesong Terhadap Bakteri Uji Penyebab Infeksi Kulit. As-Syifaa Jurnal Farmasi, 15(1), Pp.37-45.
Ngajow, M., Abidjulu, J. And Kamu, V.S., 2013. Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia Pinnata) Terhadap Bakteri Staphylococcus Aureus Secara In Vitro. Jurnal Mipa, 2(2), Pp.128-132.
Downloads
Published
Issue
Section
License
OPEN ACCESS POLICY
The Journal Microbiology Science (JMS) is an open access journal, with the aim of informing and publishing research results for development of science and technology, especially in the field of microbiology freely available to the public supports a greater global exchange of knowledge. All articles can be downloaded for free. Articles published in the Journal are Open Access articles distributed under Creative Commons Attribution-ShareAlike 4.0 International License.
The benefits of open access for authors include:
- Open access articles are freely available online and permanently.
- Authors hold copyright to their work..
- Increased visibility and readership.
- No spatial restrictions.
For readers to become writers, There is no space limit for additional material including images, extensive data, or video recordings. Allows readers to read, downloads, quotes, distributes, prints, searches, or links to the full text and allows readers to use them for other lawful purposes.
COPYRIGHT
Submission of a manuscript implies that the submitted work has not been published before (except as part of a thesis, report, or abstract), is not under consideration for publication elsewhere, and has been approved by all co-authors. Upon acceptance, authors retain copyright to their work and grant the Journal Microbiology Science (JMS) a license to distribute the work under the Creative Commons Attribution-ShareAlike 4.0 International License. This license permits others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, including commercial use), provided proper credit is given to the original authors, changes are indicated, and any derivative works are distributed under the same license. Authors are encouraged to manage intellectual property rights, including patents, for any new inventions prior to publication.
DISCLAIMER
No responsibility is assumed by publisher and co-publishers, nor by the editors for any injury and/or damage to persons or property as a result of any actual or alleged libelous statements, infringement of intellectual property or privacy rights, or products liability, whether resulting from negligence or otherwise, or from any use or operation of any ideas, instructions, procedures, products or methods contained in the material therein
Journal Microbiology Science by Faculty of Pharmacy Universitas Muslim Indonesia is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
