Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection
DOI:
https://doi.org/10.56711/jms.v4i1.995Abstract
Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.References
Handrianto Prasetyo. Aktivitas Antibakteri Ekstrak Metanol Jamur Lingzhi (Ganoderma lucidum) terhadap Staphylococcus aureus. Journal of Pharmacy and Science. 2018; 3(1): 2527-6328.
Siswoyo UC, Fitrianingsih SP, Hazar S. Studi Literatur Potensi Antibakteri Tanaman Sawo (Manilkara zapota (L.) P. Royen) terhadap Bakteri Penyebab Infeksi Saluran Pencernaan. Bandung Conference Series:Pharmacy. 2022; 2(2):272-280
Bakhtra DDA, Jubahar J, Yusdi E. Uji Aktivitas Fraksi dari Ekstrak Daun Sambung Nyawa (Gynura procumbens (lour)merr.) terhadap Bakteri Shigella dysenteriae. In Jurnal Farmasi Higea. 2018; 10(1):10-18
Jap ALS, Widodo AD. Diare Akut yang Disebabkan oleh Infeksi. Jurnal Kedokteran Meditek. 2021; 27(3):282-288
Nofitasari F, Widya HS. Penerapan Kompres Hangat untuk Menurunkan Hipertermia pada Anak dengan Demam Typoid. In Jurnal Manajemen Asuhan Keperawatan. 2019; 3(2):282-288.
Nuha AR, Resmawan. Analisis Model Matematika Penyebaran Penyakit Kolera Dengan Mempertimbangkan Masa Inkubasi. Jurnal Ilmiah Matematika Dan Terapan. 2020; 17(2), 212–229
Zeniusa P, Ramadhian MR. Efektifitas Ekstrak Etanol Teh Hijau Dalam Menghambat Pertumbuhan Escherichia coli. Majority. 2017;7(1):26-30.
Djawaria DPA, Setiadi AP, Setiawan E. Analisis Perilaku Dan Faktor Penyebab Perilaku Penggunaan Antibiotik Tanpa Resep Di Surabaya. Media Kesehatan Masyarakat Indonesia. 2018; 14(4):406-417
Saputri R, Rakhman HA. Edukasi Dagusibu Antibiotik Yang Tepat Untuk Mencegah Resistensi Bakteri Dikelurahan Basirih. Majalah Cendekia Mengabdi. 2023;1(2): 57-60
Islamiyah AN, Septiani V, Margayani, Margayani E. Perilaku Dan Pengetahuan Penggunaan Antibiotik Pada Populasi Masyarakat Bandung Raya. Pharmacoscrip. 2023; 6(1):53-67
Setyarini AIR., et al. Farmakologi Dasar. Jakarta : Gramedia. 2022
Jati NK, Agung TP, Mursiti S. Isolasi, Identifikasi, Dan Uji Aktivitas Antibakteri Senyawa Alkaloid Pada Daun Pepaya. Jurnal Mipa. 2019; 42(1):1–6.
Haeria, Nursyamsi D, Risnawati H. Aktivitas Antibakteri Fraksi-Fraksi Daun Bidara (Ziziphus mauritiana) Antibacterial Activity Of Bidara Leaf Fractions (Ziziphus mauritiana). J.Pharm.Sci. 2018; 1(2):94-102
Sari R., Mutiara M, Inara F. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Gaharu (Aquilaria microcarpa baill.) Terhadap bakteri Staphylococcus aureus dan Proteus mirabilis Antibacterial Activity Of Ethanolic Leaves Extract Of Agarwood (Aquilaria microcarpa baill.) Against Staphylococcus aureus and Proteus mirabilis. Pharm Sci Res. 2017; 4(3):143-154
Nofiani R, Ardiningsih. Isolasi Dan Identifikasi Serta Uji Aktivitas Antibakteri Fungi Endofit Gaharu Dari Aquilaria sp. Jurnal Kimia Khatulistiwa. 2020; 8 (4):13–18.
Murdiyah S. Endophytic Fungi Of Various Medicinal Plants Collected From Evergreen Forest Baluran National Park And Its Potential As Laboratory Manual For Mycology Course. Jurnal Pendidikan Biologi Indoneisa. 2020; 3(1):64-71)
Nurung AH, Fitriana, Herwin. Penentuan Aktivitas Antibakteri Ekstrak Isolat Fungi Endofit Dari Daun Galing-Galing (Cayratia trifolia L) (Determination Of Antibacterial Activities Endophytic Fungi Isolate From Leaves Galing-Galing (Cayratia trifolia L)). As-syifaa. 2022; 14(1):11-17.
Tulak Imelyani Amba. Isolasi Fungi Endofit Daun Bidara (Ziziphus mauritiana Lam.) Sebagai Penghasil Antibakteri Dengan Metode KLT-Bioautografi. Fakultas Farmasi. Universitas Muslim Indonesia. Skripsi. 2017
Asnita A, Herwin H, Kosman R, Nurung AH. Isolasi Dan Identifikasi Fungi Endofit Batang Sesuru (Euphorbia antiquorum l.) Sebagai Penghasil Antibakteri Dengan Metode KLT-bioautografi. Jurnal Ilmiah. As-syifaa. 2021; 12 (2):144-149.
Suryaningsih V, Ferniah RS, Kusdiyantini E. Karakteristik Morfologi, Biokimia, Dan Molekuler Isolat Khamir IK-2 Hasil Isolasi Dari Jus Buah Sirsak (Annona muricata l.). Jurnal biologi. 2018; 7(1):18-25.
Ayu GSD, Hendri M. Isolasi Dan Aktivitas Antibakteri Jamur Endofit Pada Mangrove Avicennia Marina Dari Pulau Payung Kabupaten Banyuasin Sumatera Selatan. Jurnal Penelitian Sain. 2021; 23(3):125-133.
Rusli, Kosman R, Melinda P. Penelusuran Fungi Endofit Pada Daun Kopasanda (Chromolaena odorata l.) Yang Berpotensi Sebagai Penghasil Antibakteri Terhadap Bakteri Penyebab Infeksi Kulit. As-syifaa. 2020; 12(1):64-69.
Majoumouo MS, Tincho MB, Kouipou Toghueo RM, Morris T, Hiss DC, Boyom FF, Mandal C. Cytotoxicity Potential Of Endophytic Fungi Extracts from Terminalia Catappa Against Human Cervical Cancer Cells. Journal of Toxicology. 2020; 20(20):1-9
Wijayati N, Astutiningsih C, Mulyani S. Transformation α-Pinena by Bacteri Pseudomonas aeruginosa ATCC 25923. Biosaintifika. 2014;6(1):24-28
Aslah AP, Widya AL, Imam J. Aktivitas Antibakteri Dan Analisis KLT-Bioautografi Dari Fraksi Daun Mengkudu (Morinda citrifolia l.). Pharmacon. 2019; 8(2):505-515
Annisa Khusnul. Penentuan Waktu Optimum Produksi Antibakteri Isolat Fungi Endofit Dari Tanaman Bidara (Ziziphus mauritiana Lam.). Skrripsi. 2023
Ernawati, Telo YM. Teknik Pengamatan Dan Identifikasi Jamur Pada Ikan Yang Dilalulintaskan Di Stasiun Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan Palembang. Jurnal Ilmiah Biologi Sriwijaya Bioscientia. 2022; 22(1):22-26
Rante H, Abd HU, Dominggus PM. Isolasi FungiEendofit Dari Daun Asam Jawa (Tamarindus indica L.) Sebagai Penghasil Senyawa Antibakteri. Original Article MFF. 2021; 25(2):66-68
Widjajanti H, Nurnawati E, Muharni, Zahwa ED. Optimization Of Antibacterial Production Of Endophytic Fungi With Various Sources of C, N, and pH Using The Response Surface Methodology. In Science And Technology Indonesia. 2020; 7(2):149-157
Abdullah M, Fitriana, Maryam S. Uji aktivitas antioksidan isolat fungi endofit daun galing-Galing (Cayratia trifolia l.) Dengan metode 1,1-diphenyl-2-picrylhydrazil (dpph). As-Syifaa. 2020; 12(2):117-122
Sari AIP. Aktivitas Antibakteri Isolat Fungi Endofit Dari Tanaman Bidara (Ziziphus mauritiana Lam.) Secara KLT-Bioautografi. Skripsi. 2023
Saputera MMA, Marpaung TWA, Ayuchecaria N. Konsentrasi Hambat Minimum (KHM) Kadar Ekstrak Etanol Batang Bajakah Tampala (Spatholobus littoralis hassk) Terhadap Bakteri Escherichia coli Melalui Metode Sumuran. Jurnal Ilmiah Manuntung. 2019; 5(2):167–173.
Pertiwi FD, Rezaldi F, Puspitasari R. Aktivitas Antibakteri Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Terhadap Bakteri Staphylococcus epidermidis. Jurnal Ilmiah Biosaintropis. 2020; 7(2):57-68
Prabasari, P.A., Sumarya, I.M, & Juliasih, N. K. A. Daya Hambat Ekstrak Lidah Buaya (Aloe barbadensis miller) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Secara In Vitro. Jurnal Widya Biologi. 2019; 1(1):23-32
Downloads
Published
Issue
Section
License
OPEN ACCESS POLICY
The Journal Microbiology Science (JMS) is an open access journal, with the aim of informing and publishing research results for development of science and technology, especially in the field of microbiology freely available to the public supports a greater global exchange of knowledge. All articles can be downloaded for free. Articles published in the Journal are Open Access articles distributed under Creative Commons Attribution-ShareAlike 4.0 International License.
The benefits of open access for authors include:
- Open access articles are freely available online and permanently.
- Authors hold copyright to their work..
- Increased visibility and readership.
- No spatial restrictions.
For readers to become writers, There is no space limit for additional material including images, extensive data, or video recordings. Allows readers to read, downloads, quotes, distributes, prints, searches, or links to the full text and allows readers to use them for other lawful purposes.
COPYRIGHT
Submission of a manuscript implies that the submitted work has not been published before (except as part of a thesis, report, or abstract), is not under consideration for publication elsewhere, and has been approved by all co-authors. Upon acceptance, authors retain copyright to their work and grant the Journal Microbiology Science (JMS) a license to distribute the work under the Creative Commons Attribution-ShareAlike 4.0 International License. This license permits others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, including commercial use), provided proper credit is given to the original authors, changes are indicated, and any derivative works are distributed under the same license. Authors are encouraged to manage intellectual property rights, including patents, for any new inventions prior to publication.
DISCLAIMER
No responsibility is assumed by publisher and co-publishers, nor by the editors for any injury and/or damage to persons or property as a result of any actual or alleged libelous statements, infringement of intellectual property or privacy rights, or products liability, whether resulting from negligence or otherwise, or from any use or operation of any ideas, instructions, procedures, products or methods contained in the material therein
Journal Microbiology Science by Faculty of Pharmacy Universitas Muslim Indonesia is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
