ANTIBACTERIAL ACTIVITY OF PATCHOULI OIL REFINERY WASTE AGAINTS STAPHYLOCOCCUS AUREUS BACTERIA

Authors

  • Sarmila Sarmila Faculty of Mathematics and Natural Science, Tadulako University, Palu, Central Sulawesi
  • Abd. Rahman Razak Faculty of Mathematics and Natural Science, Tadulako University, Palu, Central Sulawesi
  • Jamaluddin Jamaluddin Faculty of Mathematics and Natural Science, Tadulako University, Palu, Central Sulawesi

DOI:

https://doi.org/10.56711/jms.v2i1.821

Abstract

Patchouli oil refining waste is biomass or organic waste which has chemical contect so that it has antibacterial potential. This study aims to determine the antibacterial activity of patchouli oil refining waste against Staphylococcus aureus and knowing the groups of compounds identified as having antibacterial activity after the TLC Bioautography test was carried out. The sample was extracted using the maceration method with 96%  ethanol solvent. Antibacterial activity testing was carried out with a concentration variant of 50%, 25% and 10% with the well method. Thin layer Chromatography (TLC) using n-hexane : ethyl acetate (3:1) elucants. TLC Bioautography using contact bioautography method. From the research that has been done, it is found that the ethanol extract of patchouli oil refining can inhibit Staphylococcus aureus which has the largest diameter of resistance with a concentration of 50% which is categorized as very strong. The results of the TLC Bioautography study showed the saponin compound class after being sprayed with H2SO4 10% reagent, it was found that the ethanol extract of patchouli oil refined pulp has antibacterial activity against Staphylococcus aureus and compounds to play a role in antibacterial activity are saponins.

References

Setya NH, Aprilia B, Mahfud. Proses Pengambilan Minyak Atsiri Dari Daun Nilam Dengan Pemanfaatan Gelombang Mikro (Microwave). Jurnal Teknik ITS. 2012; 1(1):25–29

Riwayati I, Setianto MS. Pemanfaatan Limbah Penyulingan Daun Nilam Sebagai Bahan Bakar Alternatif Melalui Pembuatan Briket. Momentum. 2010; 6(2):1–4

Yuliani S, Usmiati S, Nurdjannah N. Efektivitas Lilin Penolak Lalat (Repelen) Dengan Bahan Aktif Limbah Penyulingan Minyak Nilam. Journal Penelitian Pascapanen Pertanian. 2005; 2(1):1–10

Sanarto S. Mekanisme Resistensi Antimikroba Pada Bakteri Penyebab Infeksi Kulit. In: Skin Infection it’s Amust Know Disease. Malang: Universitas Brawijaya Press (UB Press). 2016, p. 25

Adriani M, Wirjatmadi B. Gizi Dan Kesehatan Balita: Peranan Mikro Zinc Pada Pertumbuhan Balita. Jakarta: KENCANA Prenada Media Group. 2014

Widowati R, Handayani S, Lasdi I. Aktivitas Antibakteri Minyak Nilam (Pogostemon Cablin) Terhadap Beberapa Spesies Bakteri Uji. Pro-Life. 2019; 6 Nomor 3:244

Novaldi N, Prismawiryanti P, Hardi J, Ys H. Mikroenkapsulasi Ekstrak Ampas Jahe Merah Dari Hasil Pemisahan Minyak Jahe Merah. KOVALEN: Jurnal Riset Kimia. 2019; 5(1):17–23

Afni N, Said N, Yuliet Y. Uji Aktivitas Antibakteri Pasta Gigi Ekstrak Biji Pinang (Areca catechu L.) terhadap Streptococcus mutans dan Staphylococcus aureus. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal). 2015; 1(1):48–58

Sumarsih S. Untung Besar Usaha Bibit Jamur Tiram. I. Jakarta: Penebar Swadaya

Bakhtra, Dwi DA; Jubahar, Junuarty; Yusdi E. Uji Aktivitas Fraksi Dari Ekstrak Daun Sambung Nyawa (Gynura procumbens (Lour) Merr.) Terhadap Bakteri Shigella dysenteriae. Jurnal Farmasi Higea. 2018; 10(1):10–18

Rahmadani F. Uji Aktivitas Antibakteri Dari Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea Coromandelica) Terhadap Bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Pseudomonas aeruginosa. Skripsi. Jakarta. 2015

Usman S, Ibrahim I. Uji Aktivitas Senyawa Bioaktif Antimikroba Dari Ekstrak Daun Sembukan (Paederia foetida L.) Pada Bakteri Staphylococcus aureus Dengan Metode Bioautografi. Media Farmasi. 2019; 13(2):42

Junianti J. Uji Aktivitas Antibakteri Ekstrak Daun Sumambu (Hyptiscatita Jacq.) Terhadap Bakteri Escherichia coli dan Staphylococcus aureus. Skripsi. Palu. 2018

Lasut MRC, Fatimawali F, Antasionasti I. Uji Daya Hambat Nanopartikel Ekstrak Rimpang Lengkuas Merah (Alpinia purpurata K. Schum) terhadap Pertumbuhan Bakteri Klebsiella pneumoniae Isolat Urin pada Penderita Infeksi Saluran Kemih Resisten Antibiotik Ciprofloxacin. Pharmacon. 2019; 8(4):870

Djide MN, Sartini. Analisis Mikrobiologi Farmasi. Makassar: Laboratorium Mikrobiologi Farmasi Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Hasanuddin. 2005

Wahyulianingsih W, Handayani S, Malik Abd. Penetapan Kadar Flavonoid Total Ekstrak Daun Cengkeh (Syzygium aromaticum (L.) Merr & Perry). Jurnal Fitofarmaka Indonesia. 2016; 3(2):188–193

Suryanto E. Fitokimia Antioksidan. Surabaya: Putra Media Nusantara. 2012

Hasnaeni, Wisdawati, Usman S. Pengaruh Metode Ekstraksi Terhadap Rendemen Dan Kadar FenolikEkstrak Tanaman Kayu Beta-Beta (Lunasia Amara Blanco). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal). 2019; 5(2):175–182

Misna M, Diana K. Aktivitas Antibakteri Ekstrak Kulit Bawang Merah (Allium cepa L.) Terhadap Bakteri Staphylococcus aureus. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal). 2016; 2(2):138–144

Paputungan WA, Lolo WA, Siampa JP. Aktivitas Antibakteri Dan Analisis Klt-Bioautografi Dari Fraksi Biji Kopi Robusta (Coffea canephora Pierre Ex A. Froehner). Pharmacon. 2019; 8(3):516

Ajizah A. Sensitivitas Salmonella Typhimurium Terhadap Ekstrak Daun Psidium guajava L. Sensitivitas Salmonella Typhimuriumterhadap Ekstrak Daun Psidiumguajava L. 2004; 1:31–38

Jangnga ID, Kambaya PP, Kosala K. Uji Aktivitas Antibakteri Dan Analisis Bioautografi Kromatografi Lapis Tipis Ekstrak Etanol Daun Srikaya (Annona squamosa L) terhadap Enterococcus faecalis Secara In Vitro. ODONTO: Dental Journal. 2018; 5(2):102

Khan MI et al. Green Tea Seed Isolated Saponins Exerts Antibacterial Effects against Various Strains of Gram Positive and Gram-Negative Bacteria, a Comprehensive Study in Vitro and in Vivo. Evidence-based Complementary and Alternative Medicine.; 2018. DOI: 10.1155/2018/3486106

Nuriyah B. Skrining Aktivitas Antibakteri Ekstrak Etanol 70% Dari Beberapa Daun Tanaman di Indonesia Terhadap Bakteri Salmonella Typhi Serta Bioautografinya. Skripsi. Surakarta. 2016

Rahman FA, Haniastuti T, Utami TW. Skrining Fitokimia Dan Aktivitas Antibakteri Ekstrak Etanol Daun Sirsak (Annona muricata L.) Pada Streptococcus Mutans ATCC 35668. Majalah Kedokteran Gigi Indonesia. 2017; 3(1):1

Downloads

Published

2022-04-04

Issue

Vol. 2 No. 1 (2022)

Section

Articles

License

OPEN ACCESS POLICY

The Journal Microbiology Science (JMS) is an open access journal, with the aim of informing and publishing research results for development of science and technology, especially in the field of microbiology freely available to the public supports a greater global exchange of knowledge. All articles can be downloaded for free. Articles published in the Journal are Open Access articles distributed under Creative Commons Attribution-ShareAlike 4.0 International License. 

The benefits of open access for authors include:

  • Open access articles are freely available online and permanently.
  • Authors hold copyright to their work..
  • Increased visibility and readership.
  • No spatial restrictions.

For readers to become writers, There is no space limit for additional material including images, extensive data, or video recordings. Allows readers to read, downloads, quotes, distributes, prints, searches, or links to the full text and allows readers to use them for other lawful purposes.

COPYRIGHT

Submission of a manuscript implies that the submitted work has not been published before (except as part of a thesis, report, or abstract), is not under consideration for publication elsewhere, and has been approved by all co-authors. Upon acceptance, authors retain copyright to their work and grant the Journal Microbiology Science (JMS) a license to distribute the work under the Creative Commons Attribution-ShareAlike 4.0 International License. This license permits others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, including commercial use), provided proper credit is given to the original authors, changes are indicated, and any derivative works are distributed under the same license. Authors are encouraged to manage intellectual property rights, including patents, for any new inventions prior to publication.

DISCLAIMER

No responsibility is assumed by publisher and co-publishers, nor by the editors for any injury and/or damage to persons or property as a result of any actual or alleged libelous statements, infringement of intellectual property or privacy rights, or products liability, whether resulting from negligence or otherwise, or from any use or operation of any ideas, instructions, procedures, products or methods contained in the material therein

 

 
Journal Microbiology Science by Faculty of Pharmacy Universitas Muslim Indonesia is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.