Potential of Antibacterial Producing Rhizosphere Bacteria from Porang Roots (Amorphophallus muelleri Blume) against Gastrointestinal Pathogens

Authors

  • Siska Nuryanti Indonesian Muslim University of Makassar image/svg+xml
  • Ayyub Harly Nurung Indonesian Muslim University of Makassar image/svg+xml
  • Andi Al Kahfi Akihito mr Indonesian Muslim University of Makassar image/svg+xml

DOI:

https://doi.org/10.56711/jms.v5i2.1342

Keywords:

Rhizospheric Bacteria, Amorphophallus muelleri Blume, MIC, MBC, Gastrointestinal Infections

Abstract

Increasing antibiotic resistance in gastrointestinal pathogens such as Escherichia coli, Shigella dysenteriae, and Vibrio cholerae encourages the search for new antimicrobial agents. This study aims to evaluate the antibacterial potential of rhizosphere bacteria from porang roots (Amorphophallus muelleri Blume) against the three pathogens through in vitro tests. Isolation was carried out from rhizosphere soil using a serial dilution technique. Eleven isolates were characterized morphologically and tested for antibacterial activity using the agar diffusion method. Two selected isolates (IBRAM-2 and IBRAM-11) were fermented and extracted with ethyl acetate. Antibacterial activity was evaluated using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests with the microdilution and drop plate methods, each with three replications and chloramphenicol as a positive control. The results showed an inhibition zone of 13.2–18.7 mm. The MIC value is 25,000 ppm, while the MBC value ranges from 25,000–50,000 ppm. Although it shows antibacterial activity, the high concentration required indicates weak antibacterial potential.

Author Biographies

  • Siska Nuryanti, Indonesian Muslim University of Makassar
    Department of Pharmaceutical Microbiology
  • Ayyub Harly Nurung, Indonesian Muslim University of Makassar
    Department of Pharmaceutical Microbiology
  • Andi Al Kahfi Akihito mr, Indonesian Muslim University of Makassar
    Undergraduate Program in Pharmacy, Faculty of Pharmacy

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Published

2025-11-21

Issue

Vol. 5 No. 2 (2025): Volume 5(2) 2025

Section

Articles

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