Antibacterial Activity of Ethanolic Extract of Pandanus Nut (Pandanus julianettii) Gastrointestinal Bacteria Using The Agar Diffusion Method
DOI:
https://doi.org/10.56711/jms.v5i2.1334Keywords:
Key Words, Pandanus julianetii, Gastrointestinal Infection, Antibacterial, Agar Diffusion Method.Abstract
Gastrointestinal infections are a major global health concern, often caused by pathogenic bacteria. This study aimed to evaluate the antibacterial activity of the ethanolic extract of Pandanus nut (Pandanus julianettii) against gastrointestinal pathogens using the agar diffusion method. The Minimum Inhibitory Concentration (MIC) of the extract was determined to be 6.4% for Shigella dysenteriae, Salmonella typhi, and Escherichia coli, and 3.2% for Vibrio cholerae. The Minimum Bactericidal Concentration (MBC) for all tested bacteria was 12.8%. Antibacterial activity analysis revealed that at the 12.8% concentration, the largest inhibition zones were observed: 22.68 mm for Salmonella typhi, 22.20 mm for Shigella dysenteriae, 21.26 mm for Escherichia coli, and 20.80 mm for Vibrio cholerae. These findings indicate that the ethanolic extract of Pandanus julianettii possesses strong antibacterial properties and holds potential as a natural therapeutic agent for managing gastrointestinal infections.
References
Rabbana R, Kosman R, Nuryanti S. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelica (Houtt.) Merr.) terhadap Bakteri Penyebab Infeksi Saluran Pencernaan dengan Metode Difusi Agar. Makassar Pharmaceutical Science Journal. 2023;1(2):66–75. Available at: https://journal.farmasi.umi.ac.id/index.php/mpsj
Rusli, Fajri MD, Sari ASN, Asmasari N. Jamur Endosimbion si Bintang Laut (Asterias forbesi) sebagai Alternatif Antibakteri Baru pada Bakteri Penyebab Infeksi Saluran Pencernaan. Jurnal Ilmiah As-Syifaa. 2016;8(2):1–9. Available at: https://doi.org/10.33096/jifa.v8i2.199
Kristiana T. Pendahuluan Sistem Pencernaan pada Tubuh Manusia: Fungsi Penghancuran Makanan dan Peran Enzim. Jurnal Ilmu Komputer. 2018;14(2):65–80.
Siswoyo UC, Fitrianingsih SP, Hazar S. Studi Literatur Potensi Antibakteri Tanaman Sawo (Manilkara zapota (L.) P. Royen) terhadap Bakteri Penyebab Infeksi Saluran Pencernaan. Bandung Conference Series: Pharmacy. 2022;2(2):272–280. Available at: https://doi.org/10.29313/bcsp.v2i2.4111
Zebua LI, Purnamasari V. Oil of Pandan Kelapa Hutan (Pandanus jiulianettii Martelli): Physicochemical Properties, Total Phenols, Total Carotene, Vitamin E, and Antioxidant Activity. Jurnal Biologi Udayana. 2018;21(2):71–77. Available at: https://doi.org/10.24843/jbiounud.2017.vol21.i02.p05
Puspasari S, Nurhamidah N, Amir H. Uji Sitotoksik dan Aktivitas Antibakteri Ekstrak Daun Pandan Laut (Pandanus odorifer) terhadap Bakteri Staphylococcus aureus. Alotrop. 2020;4(1):42–50. Available at: https://doi.org/10.33369/atp.v4i1.13708
Fitriana K, Irwandi, Muslihin AM. Formulasi dan Uji Karakteristik Stabilitas Fisik Sediaan Gel Sunscreen Ekstrak Etanol Buah Merah (Pandanus conoideus). Jurnal Etnofarmasi. 2023;1(1):30–37.
Pujiastuti E, El’Zeba D. Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70% dan 96% Kulit Buah Naga Merah (Hylocereus polyrhizus) dengan Spektrofotometri. Cendekia Journal of Pharmacy. 2021;5(1):28–43. Available at: https://doi.org/10.31596/cjp.v5i1.131
Hibai ARY, Herwin, Kosman R. Antibacterial Activity Assay of Ethanolic Extract of Bulbs Sticky Taro (Colocasia esculenta) Using TLC-Bioautography. Jurnal Ilmiah As-Syifaa. 2015;7(1):76–84. Available at: https://doi.org/10.33096/jifa.v7i1.24
Maryam S, Juniasti S, Kosman R. Uji Aktivitas Antibakteri Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi L.) Asal Kota Watampone. Jurnal Ilmiah As-Syifaa. 2015;7(1):60–69. Available at: https://doi.org/10.33096/jifa.v7i1.21
Azis MA, Wardani TS, Fitriawati A. Uji Aktivitas Antibakteri Ekstrak dan Fraksi N-Heksan, Etil Asetat, dan Air Kulit Buah Naga Putih (Hylocereus undatus) terhadap Staphylococcus epidermidis ATCC 12238. OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan. 2024;2(4):41–56. Available at: https://doi.org/10.61132/obat.v2i4.478
Hasnaeni, Wisdawati, Usman S. Pengaruh Metode Ekstraksi terhadap Rendemen dan Kadar Fenolik Ekstrak Tanaman Kayu Beta-Beta (Lunasia amara Blanco). Jurnal Farmasi Galenika. 2019;5(2):175–182.
Herwin, Maryam S. Aktivitas Antimikroba Ekstrak Daun Teh Hijau (Camellia sinensis) dan Jati Belanda (Guazuma ulmifolia Lamk.) terhadap Mikroba Patogen secara KLT-Bioautografi. Jurnal Ilmiah As-Syifaa. 2017;9(1):92–98.
Herwin, Sari ZP, Nuryanti S. Aktivitas Antibakteri Ekstrak Etanol Daun dan Ampas Teh Hijau (Camellia sinensis L.) terhadap Bakteri Penyebab Jerawat (Propionibacterium acnes dan Staphylococcus epidermidis) secara Difusi Agar. As-Syifaa Jurnal Farmasi. 2018;10(2):247–254.
Rusli, Mahmud MF, Kosman R. Aktivitas Antibakteri Ekstrak Etanol Daun Bawang Dayak (Eleutherine palmifolia (L.) Merr.) terhadap Bakteri Penyebab Infeksi Kulit dengan Metode Difusi Agar. Jurnal Novem Medika Farmasi. 2023;1(3):42–53.
Wendersteyl NV, Wewengkang DS, Abdullah SS. Uji Aktivitas Antimikroba dari Ekstrak dan Fraksi Ascidian Herdmania momus dari Perairan Pulau Bangka Likupang terhadap Pertumbuhan Mikroba Staphylococcus aureus, Salmonella typhimurium, dan Candida albicans. Pharmacon. 2021;10(1):706.
Arma U, Mailiza F, Anggestia W, Azira FN. Uji Zona Hambat Ekstrak Metanol Teripang Pandan (Stichopus variegatus Semper) terhadap Bakteri Porphyromonas gingivalis. Clinical Dental Journal UGM. 2024;10(1):1–8.
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