Antibacterial Activity of N-Hexane and Ethyl Acetate Fractions Of Pandanus Nut (P. Julianettii) Against Oral Pathogens
DOI:
https://doi.org/10.56711/jms.v5i2.1333Keywords:
Pandanus julianettii, Oral Infection, Antibacterial, Agar Diffusion, Streptococcus mutans, Porphyromonas gingivalis.Abstract
Oral infections caused by Streptococcus mutans and Porphyromonas gingivalis are common health problems. This in vitro study aimed to evaluate the antibacterial activity of Pandanus nut (Pandanus julianettii) with n-hexane and ethyl acetate fractions against these pathogens through agar diffusion assays. The n-hexane and ethyl acetate fractions were subjected to agar diffusion testing, with Minimum Inhibitory Concentration (MIC) values determined at 0.8% and 0.2% respectively against both bacterial strains. Subsequent Minimum Bactericidal Concentration (MBC) testing revealed values of 6.4-12.8% for n-hexane fraction and 3.2-6.4% for ethyl acetate fraction. In agar diffusion assays, the n-hexane fraction at 12.8% concentration produced inhibition zones of 15.54±0,20 mm on S. mutans and 15.46±0,35 mm on P. gingivalis. The ethyl acetate fraction showed significantly larger inhibition zones of 18.76±0,79 mm against S. mutans and 15.59±0,42 mm against P. gingivalis at the same concentration. These findings demonstrate that both n-hexane and ethyl acetate fractions of Pandanus nut possess substantial antibacterial activity against oral pathogens.References
Hadinata D, Lutfi B. Patofisiologi. Tasikmalaya: Edu Publisher; 2022.
Rafiei M, Kiani F, Sayehmiri F, Sayehmiri K. Study of Porphyromonas gingivalis in Periodontal Diseases: A Systematic Review and Meta-Analysis. Med J Islam Repub Iran. 2017;31(1):355–362.
Goering RV, Dockrell HM, Zuckerman M, Chiodini PL. MIMS Medical Microbiology and Immunology. Singapore: Elsevier; 2019.
World Health Organization (WHO). Global Status Report on Oral Health 2022. Geneva: WHO; 2022. Accessed 30 Nov 2024.
Kementerian Kesehatan Republik Indonesia (BKPK). Survei Kesehatan Indonesia. Jakarta: Kementerian Kesehatan; 2023.
Priamsari MR, Wibowo AC. Aktivitas Antibakteri Ekstrak Perasan Daun Mengkudu (Morinda citrifolia L.) terhadap Escherichia coli secara In Vitro. Jurnal Riset Kefarmasian Indonesia. 2020;2(1):26–34.
Seran L, Herak R, Missa H. Pembuktian Kemampuan Antibakteri Ekstrak Daun dan Kulit Jarak Pagar (Jatropha curcas) terhadap Bakteri Staphylococcus aureus secara In Vitro dalam Pembelajaran dengan Metode PBL. Jurnal Studi Guru dan Pembelajaran. 2020;3(1):39–50.
Daud NS, Arni DP, Idris SA, Saehu MS. Uji Aktivitas Antibakteri Ekstrak Batang Meistera chinensis terhadap Escherichia coli ATCC 35218. Jurnal Warta Farmasi. 2023;12(1):8–18.
Zebua LI, Purnamasari V. Minyak Pandan Kelapa Hutan (Pandanus jiulianettii Martelli): Sifat Fisikokimia, Total Fenol, Total Karoten, Vitamin E, dan Aktivitas Antioksidan. Jurnal Biologi Udayana. 2017;21(2):71–77.
Kagoya B. Tanaman Woromo Papua. Accessed 30 Nov 2024. Available from: https://woromopapua.wordpress.com/category/buku/.
Condro N, Lada YG. Skrining Fitokimia Pandanus julianetii sebagai Sumber Pangan Fungsional Lokal Papua. Jurnal Ilmu Pangan dan Hasil Pertanian. 2020;4(1):74–82.
Nurdyansyah F, Widyastuti DA, Retnowati EI. Studi Senyawa Metabolit Sekunder dan Aktivitas Antibakteri Ekstrak Sirsak (Annona muricata Linn.). Seminar Nasional Hasil Penelitian (SNHP). 2021;2:325–334.
Fitriana K, Muslihin AM, Irwandi. Formulasi dan Uji Karakteristik Stabilitas Fisik Sediaan Gel Sunscreen Ekstrak Etanol Buah Merah (Pandanus conoideus). Jurnal Etnofarmasi Unimuda. 2023;1(1):30–37.
Pujiastuti E, El’Zeba D. Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70% dan 96% Buah Naga Merah (Hylocereus polyrhizus) dengan Spektrofotometri. Cendekia Journal of Pharmacy. 2021;5(1):28–43.
Herwin, Nurung AH, Ambo NI, Naid T. Identifikasi Komponen Kimia Ekstrak Etanol Daun Pacar Kuku (Lawsonia inermis L.) sebagai Antibakteri dan Antioksidan. Journal Microbiology Science. 2022;2(1):26–33.
Rusli, Prayitno D. Isolasi dan Identifikasi Golongan Kimia Aktif Antibakteri Ekstrak N-Butanol Sawo Manila (Achras zapota Linn.) asal Kota Palopo. As-Syifaa Jurnal Farmasi. 2017;9(2):201–210.
Asnita, Kosman R, Herwin, Nurung AH. Isolasi dan Identifikasi Fungi Endofit Batang Sesuru (Euphorbia antiquorum L.) sebagai Penghasil Antibakteri dengan Metode KLT-Bioautografi. As-Syifaa Jurnal Farmasi. 2020;12(2):144–149.
Yahya FA, Herwin, Fitriana. Antibacterial Activity of N-Hexan and Ethyl Acetate Fractions of Gaharu (Aquilaria malaccensis) Leaves Against Bacteria That Cause Infection. Journal Microbiology Science. 2024;4(2):208–218.
Fitriana, Amirah S, Rahman S, Sukmawati. Aktivitas Antimikroba Ekstrak Etanol Batang Wole Woe terhadap Mikroba Patogen Menggunakan Difusi Agar. As-Syifaa Jurnal Farmasi. 2023;15(1):54–60.
Herwin, Sari ZP, Nuryanti S. Aktivitas Antibakteri Ekstrak Etanol Daun dan Ampas Teh Hijau (Camellia sinensis L.) terhadap Bakteri Penyebab Jerawat (Propionibacterium acnes dan Staphylococcus epidermidis) secara Difusi Agar. Jurnal Ilmiah As-Syifaa. 2018;10(2):247–254.
Herwin, Mile FA. Aktivitas Antibakteri Ekstrak Etanol Batang Phytocrene macrophylla Blume. Jurnal Ilmiah As-Syifaa. 2017;9(2):165–172.
Imawati MF, Indriasari C, Azsrina GN. Studi Variasi Metode Pengeringan terhadap Skrining Fitokimia Simplisia Krokot Magenta (Portulaca grandiflora). Jurnal Mahasiswa Ilmu Farmasi dan Kesehatan. 2023;1(3):181–188.
Azis MA, Wardani TS, Fitriawati A. Uji Aktivitas Antibakteri Ekstrak dan Fraksi N-Heksan, Etil Asetat, Air Kulit Buah Naga Putih (Hylocereus undatus) terhadap Staphylococcus epidermidis ATCC 12238. OBAT: Jurnal Riset Ilmu Farmasi dan Kesehatan. 2024;2(4):41–56.
Kusuma AE, Aprileili DA. Pengaruh Jumlah Pelarut terhadap Rendemen Ekstrak Daun Katuk (Sauropus androgynus L. Merr). SITAWA: Jurnal Farmasi Sains dan Obat Tradisional. 2022;1(2):125–135.
Alviola AB, Amin A, Mun’im A, Radji M. Rasio Nilai Rendemen dan Lama Ekstraksi Maserat Etanol Daging Buah Burahol (Stelecocharpus burahol) Berdasarkan Cara Preparasi Simplisia. Makassar Natural Product Journal. 2023;1(3):176–184.
Aliwu I, Rorong JA, Suryanto E. Skrining Fitokimia dan Uji Efek Sedatif Pelarut dari Daun Takokak (Solanum torvum Swartz) pada Tikus Putih Galur Wistar. Chemistry Progress Journal. 2020;13(1):6–10.
Syafa’ah N, Rubiyanti R, Aji N. Pengaruh Pelarut Campur Etil Asetat dan N-Heksan terhadap Rendemen dan Golongan Senyawa Ekstrak Biji Alpukat. Jurnal Media Informasi. 2019;15(1):54–64.
Rusli, Mahmud MF, Kosman R. Aktivitas Antibakteri Ekstrak Etanol Daun Bawang Dayak (Eleutherine palmifolia (L.) Merr.) terhadap Bakteri Penyebab Infeksi Kulit dengan Metode Difusi Agar. Jurnal Novem Medika Farmasi. 2023;1(3):42–53.
Arma U, Mailiza F, Anggestia W, Azira FN. Uji Zona Hambat Ekstrak Metanol Teripang Pandan (Stichopus variegatus Semper) terhadap Bakteri Porphyromonas gingivalis. Clin Dent J UGM. 2024;10(1):1–8.
Daris US, Syam H, Sukainah A. Uji Daya Hambat serta Penentuan Minimum Inhibitor Concentration (MIC) dan Minimum Bactericidal Concentration (MBC) Ekstrak Daun Bidara terhadap Bakteri Patogen. Jurnal Pendidikan Teknologi Pertanian. 2023;9(2):223–234.
Wahyuni S, Yunita I, Sundari UY, Pagalla DB. Ekstraksi Bahan Alam. Padang: Gita Lentera Redaksi; 2024.
Ferdinal N, Jannah M, Afrizal. Analisis Senyawa Metabolit Sekunder dari Ekstrak Daun Mengkudu (Morinda citrifolia L.) serta Uji Aktivitas Antibakteri dan Antijamur. Jurnal Kimia Unand. 2022;14(1):1–10.
Downloads
Published
Issue
Section
License
OPEN ACCESS POLICY
The Journal Microbiology Science (JMS) is an open access journal, with the aim of informing and publishing research results for development of science and technology, especially in the field of microbiology freely available to the public supports a greater global exchange of knowledge. All articles can be downloaded for free. Articles published in the Journal are Open Access articles distributed under Creative Commons Attribution-ShareAlike 4.0 International License.
The benefits of open access for authors include:
- Open access articles are freely available online and permanently.
- Authors hold copyright to their work..
- Increased visibility and readership.
- No spatial restrictions.
For readers to become writers, There is no space limit for additional material including images, extensive data, or video recordings. Allows readers to read, downloads, quotes, distributes, prints, searches, or links to the full text and allows readers to use them for other lawful purposes.
COPYRIGHT
Submission of a manuscript implies that the submitted work has not been published before (except as part of a thesis, report, or abstract), is not under consideration for publication elsewhere, and has been approved by all co-authors. Upon acceptance, authors retain copyright to their work and grant the Journal Microbiology Science (JMS) a license to distribute the work under the Creative Commons Attribution-ShareAlike 4.0 International License. This license permits others to share (copy and redistribute the material in any medium or format) and adapt (remix, transform, and build upon the material for any purpose, including commercial use), provided proper credit is given to the original authors, changes are indicated, and any derivative works are distributed under the same license. Authors are encouraged to manage intellectual property rights, including patents, for any new inventions prior to publication.
DISCLAIMER
No responsibility is assumed by publisher and co-publishers, nor by the editors for any injury and/or damage to persons or property as a result of any actual or alleged libelous statements, infringement of intellectual property or privacy rights, or products liability, whether resulting from negligence or otherwise, or from any use or operation of any ideas, instructions, procedures, products or methods contained in the material therein
Journal Microbiology Science by Faculty of Pharmacy Universitas Muslim Indonesia is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
