Exploring The Antibacterial Potential of Aqueous and Ethanol Extracts from Bawang Dayak (Eleutherine palmifolia (L.) Merr) Against Skin Infectious Bacteria Through KLT-Bioautography
DOI:
https://doi.org/10.56711/jms.v4i2.1131Abstract
Dayak onion plants (Eleutherine palmifolia (L.) Merr) have chemical compounds that have potential as antibacterials such as alkaloids, glycosides, flavonoids, phenolics, steroids and tannins. This study aims to determine the antibacterial activity of water extracts and ethanol extracts of dayak onion bulbs (Eleutherine palmifolia (L.) Merr). This research was conducted with the stages of sample extraction, screening of test bacteria, bioautogram profile testing and antibacterial activity by KLT-Bioautography method. Screening test results at a concentration of 1% against test bacteria Propionibacterium acnes, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis were able to inhibit the growth of test bacteria. Then continued with KLT-Bioautography testing using n-hexan: ethyl acetate (2: 1) eluent. The KLT-Bioautography test results showed that there was 1 active spot with an Rf value of 0.63 in the water extract and an Rf value of 0.60 in the ethanol extract against Pseudomonas aeruginosa, Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis bacteria. Based on the research, water extract and ethanol extract of dayak onion bulb (Eleutherine palmifolia (L.) Merr) have potential as antibacterial.
Keywords: Antibacterial, KLT-Bioautography, Dayak Onion Bulbs
References
Sugiharti RJ, Halimah I, Mahendra I, Sriyani ME. Pencitraan Di Kedokteran Nuklir Memerlukan Sensitivitas Yang Baik. Tc-Ketokonazol Adalah Radiofarmaka Antibiotik Yang Disintesis Dengan Menandai Ketokonazol Dengan Radionuklida Teknesium-99m. Radiofarmaka Ini Diharapkan Dapat Digunakan Untuk Mendeteksi In. 2016; :71–82
Azizah M, Lingga LS, Rikmasari Y. Uji Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Seledri (Apium graviolens L.) Dan Madu Hutan Terhadap Beberapa Bakteri Penyebab Penyakit Kulit. J Penelit Sains. 2020; 22(1):37
Fitriyanti F, Abdurrazaq A, Nazarudin M. Uji Efektivitas Antibakteri Esktrak Etil Asetat Bawang Dayak (Eleutherine palmifolia Merr) Terhadap Staphylococcus aureus Dengan Metode Sumuran. J Ilm Manuntung. 2019; 5(2):174–182
Prayitno B, Mukti BH, Lagiono. Optimasi Potensi Bawang Dayak (Eleutherine Sp.) Sebagai Bahan Obat Alternatif. J Pendidik Hayati. 2018; 4(3):149–158
Fitriyanti, Ridha A, Ramadhan H. Daya Antibakteri Ekstrak Etanol 96 % Umbi Bawang Dayak ( Eleutherine americana Merr .) Terhadap Bakteri Propionibacterium acnes. 2023; 4(2):265–272
Mierza V, Nasution MP, Suryanto D. Aktivitas Antibakteri Fraksi Sisa Dari Ekstrak Etanol Umbi Bawang Sabrang (Eleutherine palmifolia Merr.). J Pharm Sci. 2021; 4(2):60–68
Arisanty A, Dewi RP. Uji Efektivitas Ekstrak Air Buah Belimbing Wuluh (Averrhoa bilimbi) Terhadap Pertumbuhan Propionibacterium acnes. Media Farm. 2018; 14(2):66
Rusli, Mahmud MF, Kosman R. Aktivitas Antibakteri Ekstrak Etanol Daun Bawang Dayak (Eleutherine palmifolia (L.) Merr) Terhadap Bakteri Penyebab Infeksi Kulit Dengan Metode Difusi Agar Antibacterial Activity of Ethanol Extract of Dayak Leeks (Eleutherine palmifolia ( L .) Merr). J Novem Med Farm. 2023; 1(3):42–53
Asnita, Kosman R, Nurung AH. Isolasi Dan Identifikasi Fungi Endofit Batang Sesuru (Euphorbia antiquorum L.) Sebagai Penghasil Antibakteri Dengan Metode KLT-Bioautografi. As-Syifaa. 2020; 12(2):144–149
Damanis FVM, Wewengkang DS, Antasionasti I. Uji Aktivitas Antioksidan Ekstrak Etanol Ascidian herdmania momus Dengan Metode DPPH (1,1-Difenil-2-Pikrilhidrazil). Pharmacon. 2020; 9(3):464
Wijaya H, Novitasari, Jubaidah S. Rendemen Ekstrak Daun Rambai Laut. J Ilm Manuntung. 2018; 4(1):79–83
Octaviani M et al. Uji Aktivitas Antimikroba Ekstrak Etanol Dari Kulit Bawang Merah (Allium cepa L.) Dengan Metode Difusi Cakram Antimicrobial Activity of Ethanol Extract of Shallot (Allium cepa L.) Peels Using the Disc Diffusion Method. Pharm Sci Res. 2019; 6(1):62–68
Hiola F, Sy Pakaya M, Akuba J. Analisis Kadar Senyawa Rhodamin B Pada Sediaan Lipstik Menggunakan Metode Spektrofotometri UV-Vis. J Syifa Sci Clin Res. 2022; 3(2):98–105
Aslah A, Lolo WA, Jayanto I. Aktivitas Antibakteri Dan Analisis KLT-Bioautografi Dari Fraksi Daun Mengkudu (Morinda citrifolia L.). Pharmacon. 2019; 8(2):505–515
Wahyuni S, Marpaung MP. Penentuan Kadar Alkaloid Total Ekstrak Akar Kuning (Fibraurea chloroleuca Miers) Berdasarkan Perbedaan Konsentrasi Etanol Dengan Metode Spektrofotometri UV-VIS. Dalt J Pendidik Kim dan Ilmu Kim. 2020; 3(2):52–61
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