Isolation Of Endophytic Fungi From Aloe Vera Against Bacteria That Cause Digestive Tract Infections By TLC-Bioautography And Agar Diffusion

Evi Rahma Ramadhana, Herwin Herwin, Siska Nuryanti

Sari


The Aloe vera plant, also known as the Lidah buaya plant, has antibacterial properties due to its secondary metabolites, which include alkaloids, flavonoids, tannins, and saponins. In this study, endophytic fungi from Lidah Buaya (Aloe vera) were isolated against bacteria that cause gastrointestinal tract infections using TLC-Bioautography and Agar Diffusion. In this study, 7 isolates of endophytic fungi were obtained, one of which was IFLB 1 which had potential as an antibacterial, purification of the endophytic fungi was carried out and then macroscopic examination was carried out on the fungi to see the colonies formed. After that, a screening test was carried out for isolates of endophytic fungi. Then the isolates that gave the best activity were reproduced and continued with the fermentation process for 14 days. The extraction results were then evaporated to obtain a thick extract and a Thin Layer Chromatography (TLC) Identification test was carried out in which the eluent used was chloroform: methanol (5:1). Then continued the TLC-Bioautographic Antibacterial Activity Test on Escherichia coli, Salmonella thypi, Shigella dysentriae and Vibrio cholerae bacteria and obtained 2 active spots with Rf1 = 0.78 and Rf2 = 0.30. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 4% for Escherichia coli with an inhibition zone diameter of 15.80 mm, Salmonella thypi with an inhibition zone diameter of 14.90 mm, Shigella dysentriae with an inhibition zone diameter of 13 .85 mm and Vibrio cholerae with an inhibition zone diameter of 14.70 mm


Teks Lengkap:

PDF

Referensi


Besung INK. Pegagan (Centella Asiatica) Sebagai Alternatif Pencegahan Penyakit Infeksi Pada Ternak. Bul Vet Udayana. 2009; 1(2):61–67

Andayasari L. Kajian Epidemiologi Penyakit Infeksi Saluran Pencernaan Yang Disebabkan Oleh Amuba Di Indonesia. Media Litbang Kesehat. 2011; 21:1–9

Baskaran C, bai VR, Velu S, Kumaran K. The Efficacy of Carica Papaya Leaf Extract on Some Bacterial and a Fungal Strain by Well Diffusion Method. Asian Pacific J Trop Dis.; 2(SUPPL2). DOI: 10.1016/S2222-1808(12)60239-4

Prihatiningtias W, Widyastuti SM, Wahyuono S. Aktivitas Antibakteri Fungi Endofit Thievalia Polygonoperdia, Isolat Dari Tumbuhan Akar Kuning (Fibraurea Chloroleuca Miers). Maj Obat Tradis. 2005; 12(14):1–7

Sari SP, Raharjo SJ. Profil Senyawa Metabolit Sekunder Pada Tanaman Lidah Buaya (Aloe Vera L) Dengan Metode Kromatografi Lapis Tipis (KLT). Akad Farm Putra Indones Malang. 2019; :1–8

Purbaya J. Mengenal Dan Memanfaatkan Khasiat Aloe Vera, URL: http://ojs.ukmc.ac.id/index.php/JOH. (2003)

Rahayu I. Aloe Barbadensis Miller Dan Aloe Chinensis Baker Sebagai Antibiotik Dalam Pengobatan Etnoveteriner Unggas Secara Invi Vitro. J Protein.; 13(1), URL: http://juke.kedokteran.unila.ac.id/index.php/majority/article/view/63/62. (2006)

Ariyanti NK, Darmayasa IBG, Sudirga SK. Daya Hambat Ekstrak Kulit Daun Lidah Buaya ( Aloe Barbadensis Miller ) Terhadap Pertumbuhan Bakteri Staphylococcus Aureus ATCC 25923 Dan Escherichia Coli ATCC 25922. J Biol. 2012; 16(1):1–4

Adriani. Aktivitas Antibakterial Fungi Endofit Caulerpa Racemosa Terhadap Bakteri Escherichia Coli Dan Staphylococcus Aureus. Pros Semin Nas Mikrobiol Kesehat dan Lingkung. 2015; (2014):11–15

Reckow, V., Widayat, W., Rijai W. Pendahuluan Bawang Dayak ( Eleutherine Palmifolia ( L .) Merr .). Lab Penelit dan Pengemb "FARMAKA Trop Fak Farm Univ Mulawarman, Samarinda, Kalimantan Timur. 2016; :20–21

Ramadhani M. Potensi Fungi Endofit Bunga Asoka (Ixora Coccinea L.) Sebagai Penghasil Antibakteri. Fak Farm Univ Muslim Indones. DOI: 10.32922/jkp.v8i1.92

Siradjuddin M. Uji Aktivitas Antibakteri Ekstrak Etanol Dan Fermentat Isolat Fungi Endofit Dari Buah Dengen (Dillenia Serrate Thunb.). Fak Farm Univ Muslim Indones., URL: http://journal.trunojoyo.ac.id/jurnalkelautan. (2018)

Mani MM et al. Antimicrobial Activity and Phytochemical Screening of Various Parts of Ixora Coccinea. J Med Plants Res. 2014; 8(10):423–429

Murdiyah S. Endophytic Fungi of Various Medicinal Plants Collected from Evergreen Forest Baluran National Park and Its Potential as Laboratory Manual for Mycology Course. JPBI (Jurnal Pendidik Biol Indones. 2017; 3(1):64–71

Fitriana F, Nurshitya E. Aktivitas Antibakteri Ekstrak Isolat Fungi Endofit Dari Akar Mangrove (Rhizophora Apiculata Blume) Secara KLT Bioautografi. J Ilm As-Syifaa. 2017; 9(1):27–36

Mahmudah FL, Atun S. Uji Aaktivitas Antibakteri Dari Ekstrak Etanol Temukunci (Boesenbergia Pandurata) Terhadap Bakteri Streptococcus Mutans. Penelit Saintek. 2017; 22(1):59–66

Rusli R, Rahmaniar D. Penelusuran Potensi Mikroba Endofit Dari Rimpang Paku Kepala Tupai (Drynaria Quercifolia J.Smith) Sebagai Penghasil Senyawa Antibiotika. J Ilm As-Syifaa. 2013; 5(2):128–139




DOI: https://doi.org/10.56711/jms.v4i1.993

Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##

Indexed by:

     


ISSN: 2808-392X | e-ISSN: 2808-3911

Editor's Address:

3rd Floor Laboratory Faculty of Pharmacy,
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

Phone: +62 821 89555447
Fax: +62411425619
E-mail: jmic.sci@umi.ac.id