ISOLATION OF SKY ROOT ENDOPHITE (Helminthostachys zeylanica (L.) Hook) PRODUCING ANTIBIOTICS DIFFERENTLY AGAR

Andi Mitra, Herwin Herwin, Siska Nuryanti

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Tunjuk Langit (Helminthostachys Zelanica (L) Hook.) is a plant with family Opioglossaceae traditionally and empirically used as antibacterial with chemical compound flavonoid and saponin. This research aims to determine antibacterial activity of endophytic fungi isolate of Tunjuk Langit (Helminthostachys Zelanica (L) Hook.) root on bacteria that cause gastrointestinal infections. The result of the isolation endophytic fungi against from Tunjuk Langit (Helminthostachys Zelanica (L) Hook.) root using PDA+C medium and purification isolate obtained 7 pure isolates and analysis macroscopic from 8 isolates (IFATL-01, IFATL-02, IFATL-03, IFATL-04, IFATL-05, IFATL-05, IFATL-06, IFATL-07 and IFATL-08) obtained different charasteristics. Production of secondary metabolites of isolate active (IFATL05) by fermentation unsing maltose yeast broth (MYB) obtained supernatant and miselia. Metabolites supernatant were extracted using ethyl acetat, so obtained extract ethyl acetate supernatan. The result of the antibacterial activity of isolate endophytic fungi against V. cholerae, S. dysenteriae and E. coli bacteria by Agar diffusion obtained the largest diameter of the inhibition zone in isolate IFATL-05 = 29.13 mm active against S. dysenteriae bacteria. Based on phytochemical screening f

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Asnita., Kosman, R., Herwin,. Nurung, H.A. (2020). Isolasi dan identifikasi fungi endofit batang sesuru (Euphorbia antiquorum L.) sebagai penghasil antibakteri dengan metode klt-biaoutografi. Jurnal Farmasi Desember 2020;12(2):144-149. ISSN : 2502-9444.

Baldwin, B.G., M.J. Sanderson, J.M. Porter, M.F. Wojciechowski, C.S. Campbell, and M.J. Donoghue. 1995. The ITS region of nuclear ribosomal DNA: A valueble source of evidence on Angiosperm phylogeney. Annual Missouri Botsnic Garden 82:247-277

Chase, M.W., D.E. Soltis, and R.G. Olmstead. 1993. Phylogenetics of seed plants: An analysis of nucleotide sequences from the plastid gene rbcL. Annual Missouri Botanic Garden 80:528-580.

Fitrya dan Muharani. (2014). Efek Hipourisemia Ekstrak Etanol Akar Tumbuhabn Tunjuk Langit (Helminthostachis zeylanica (L.) Hook.) Terhadap Mencit Jantan Galur Swiss.Traditional Medicine Journal,volume 19 No. 1.ISSN: 1410-5918

Fitriana,. Maryam, S,. Naid,T, Maryana.(2016). 'Penelusuran Fungi Endofit Sebagai Penghasil Senyawa Antibiotika Dari Daun Nanas (Ananas comosus(L) Meer)', Jurnal As-Syifaa,Vol.08(01). Hal.1-3.

Hakim, SS. (2015). Fungi Endofit: Potensi Pemanfaatannya Dalam Budidaya. Tanaman Kehutanan', Balai Kehutanan Banjarbaru,Vol. 1, no,1,hal. 2-3.

Herwin,. Baits, M., Ririn. ( 2016). Aktivitas AntiBakteri Ekstrak Etanol Daun Talas Ketan (Colocasia esculenta) Terhadap Bakteri Saphylococcus aureus DAN Salmonella thypi Secara Difusi Agar, As- Syifaa Vol 08 (01) : Hal. 69- 75, Juli 2016 ISSN : 2085-4714

Hidayati, M. ( 2011). 'uji aktivitas Anti diare Ekstrak Etanol 50% Daun Salam (Syzygumpolyanthum (Wight.) Walp.h) Terhadap Mencit Jantan yang Diinduksi Oleum Ricini', S.Farm Skripsi,Fakultas Farmasi, Universitas Muhammadiyah Surakarta, Surakarta.

Hillis, D.M., C. Moritz, and B.K. Mable.1996. Molecular Sistematic. 2 Ed. Sinauer Assocites,Massachusetts.

Huang.YC.,Hwang.TL.,Chang.VS.,Yang.YL.,Shen.CN.,Liao.WY.,Ch en.SC and Liaw. CC. 2009. Anti-inflamantory Flavonoids from the Rhizome of Helminthostachys zelanica. Natural Product,72.




DOI: https://doi.org/10.56711/jms.v3i1.952

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