OPTIMIZATION OF TEMPERATURE CELLULOSE PRODUCING BACTERIAL ISOLATES FROM DRAGON FRUIT (Hylocereus polyrhizus)

Ayyub Harly Nurung, Fairuz Diah Widowaty, Herwin Herwin, Fitriana Fitriana

Sari


Isolation of bacterial cellulose from fruits in traditional market of Makassar have been done. This objective of this research was to determine optimum temperature of isolate bacteria that produce cellulose from Dragon fruit (Hylocereus polyrhizus) using dilution agar method. Isolation of bacteria produced cellulose using Hestrin-Schramm agar (HSA) obtain 5 (five) isolate NB02, NB03, NS01 and NS02. The result of screening activity showed that isolate NB04 has a potential as bacteria produce cellulose. The temperature optimize of NB04 has been done at 25oC and 40oC. Based on research results showed that optimum temperature for NB04 was at 25oC and has highest cellulose content at 1,29 g.

Key word: Dragon fruit (Hylocereus polyrhizus), bacteria cellulose, temperature optimization


Teks Lengkap:

PDF

Referensi


Lapuz MM, Gollardo E.G, Palo M .A. The Organism and Culture Requirements, Characteristics and Identity. The Philippine J Science. 1967;

MELLIAWATI R. Pengolahan limbah kulit buah buahan menjadi selulosa oleh bakteri Acetobacter sp. RMG-2. In Masyarakat Biodiversitas Indonesia; 2015.

Herwin H, Fitriana F, Nurung AH. ISOLASI BAKTERI PENGHASIL SELULOSA DARI BUAH-BUAHAN DIPASAR TRADISIONAL MAKASSAR. Jurnal Ilmiah As-Syifaa. 2020 Jul 3;12(1).

Sarkono S, Rahman F. KEMAMPUAN PRODUKSI SELULOSA ISOLAT Gluconacetobacter xylinus ANG-29 DALAM MEDIA DASAR AIR KELAPA DAN LIMBAH [Internet]. 2017. Available from: https://www.researchgate.net/publication/322753555

Umor NA. Isolation and screening of thermo-stable cellulase enzyme fungal producer at different temperature Biobutanol production in two-stage system View project [Internet]. Article in Malaysian Journal of Analytical Sciences. 2015. Available from: https://www.researchgate.net/publication/282954578

Emil S. Untung Berlipat Dari Bisnis Buah Naga Unggul. Yogyakarta: Lili Publisher; 2011.

Römling U. Molecular biology of cellulose production in bacteria. Vol. 153, Research in Microbiology. 2002.

Chawla PR, Bajaj IB, Survase SA, Singhal RS. Microbial cellulose: Fermentative production and applications. Vol. 47, Food Technology and Biotechnology. 2009.

Agung M.B. Optimasi Produksi Selulase Dari Basillus Sp. BPPT CC RK 2 Menggunakan Metode Respon Permukaan Dengan Variasi C/N Da Waktu Fermentasi. [Indonesia]; 2012.

Ayu Saropah D, Jannah A, Maunatin A. KINETIKA REAKSI ENZIMATIS EKSTRAK KASAR ENZIM SELULASE BAKTERI SELULOLITIK HASIL ISOLASI DARI BEKATUL. Vol. 2, ALCHEMY. 2012.

Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper’s Illustrated Biochemistry, Twenty-Sixth Edition. Harper ’ s Illustrated Biochemistry. 2003.

Sethi S, Datta A, Gupta BL, Gupta S. Optimization of Cellulase Production from Bacteria Isolated from Soil. ISRN Biotechnology. 2013 Feb 19;2013:1–7.


Refbacks

  • Saat ini tidak ada refbacks.


##submission.copyrightStatement##