Antibacterial Activity of Endophytic Fungi from Bidara Roots Against Bacteria that Cause Skin Infections

Sitti Nurdayani, Fitriana Fitriana, Sitti Amirah

Sari


Endophytic fungi are fungi that grow and colonize plant tissues and are capable of producing bioactive compounds similar to those produced by their host. This study aims to determine the concentration of endophytic fungal isolates from Bidara roots (Ziziphus mauritiana Lam) that have antibacterial activity against bacteria that cause skin infections using the agar diffusion method. The research method begins with the rejuvenation of IFAZ-6 isolates, and microscopic and macroscopic observations are carried out. The isolates were fermented using Maltose Yeast Broth medium at 200 rpm for 21 days. The fermentate was extracted with an ethyl acetate solvent to obtain a thick extract. The tests carried out were minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibacterial activity tests carried out using the agar diffusion method. The results of the antibacterial activity test showed that the IFAZ-6 isolate extract at a concentration of 800 ppm showed antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes.
The statistical analysis results showed that the extract of isolate IFAZ-6 against Staphylococcus aureus and Staphylococcus epidermidis at concentrations of 800-1600 ppm and 1600–3200 ppm exhibited the same antibacterial activity. The bacterium Pseudomonas aeruginosa showed the same antibacterial activity at all tested concentrations, and the bacterium Propionibacterium acnes at concentrations of 1600–3200 ppm also exhibited the same antibacterial activity.


Teks Lengkap:

PDF

Referensi


Su, H.M. Hubungan Pengetahuan dan Perilaku Hidup Bersih dengan Penyakit Kulit di Puskesmas Makbon Kabupaten Sorong. Media Publikasi Promosi Kesehatan Indonesia (MPPKI). 2005:5(8):964-969.

Sukertiasih, N.K., Megawati, F., Meriyani, H. and Sanjaya, D.A., Studi Retrospektif Gambaran Resistensi Bakteri terhadap Antibiotik. Jurnal Ilmiah Medicamento, 2021:7(2):108-111.

Yunita, S.L., Atmadani, R.N. and Titani, M., Faktor-faktor Yang Mempengaruhi Pengetahuan Dan Perilaku Penggunaan Antibiotika Pada Mahasiswa Farmasi Universitas Muhammadiyah Malang. Pharmaceutical Journal of Indonesia,2021: 6(2):119-123.

Hasiani, V. V., Ahmad, I., & Rijai, L. Isolasi Jamur Endofit dan Produksi Metabolit Sekunder Antioksidan dari Daun Pacar (Lawsonia inermis L.). Jurnal Sains dan Kesehatan, 2015:1(4):146-153.

Murdiyah, S., Fungi endofit pada berbagai tanaman berkhasiat obat di Kawasan Hutan Evergreen Taman Nasional Baluran dan potensi pengembangan sebagai petunjuk parktikum mata kuliah mikologi. Jurnal Pendidikan Biologi Indonesia, 2017:3(1):64-71.

Tulak, I.A., Isolasi Fungi Endofit Daun Bidara (Ziziphus mauritiana L.) Sebagai Penghasil Anibakteri dengan Metode KLT-Bioautografi. Universitas Muslim Indonesia. 2017.

Deponda, R. A., Fitriana, Siska N., dan Herwin. Isolasi Fungi Endofit Kulit Buah Merah (Pandanus conoideus Lam.) yang Berpotensi sebagai Antibakteri Secara Metode KLmbT-Bioautografi. As-Syifaa Jurnal Farmasi. 2019:11(2) : 147-153

Suhartina. Kandou, F.E., Singkoh, M.F., Isolasi dan Identifikasi Jamur Endofit Pada Tumbuhan Paku Asplenium nidus. Jurnal MIPA, 2018:7(2):24-28.

Situmorang, D.A., Rozirwan, R. and Hendri, M., Isolasi dan aktivitas antibakteri jamur endofit pada mangrove Avicennia marina dari Pulau Payung Kabupaten Banyuasin Sumatera Selatan. Jurnal Penelitian Sains, 2021:23(3):125-133.

Abdullah, M. and Fitriana, F., Uji Aktivitas Antioksidan Isolat Fungi Endofit Daun Galing-Galing (Cayratia trifolia L.) dengan Metode 1, 1-Diphenyl-2-Picrylhydrazil (DPPH). Jurnal Farmasi Desember, 2020: 12(2).

Majoumouo, M.S., Tincho, M.B., Kouipou Toghueo, R.M., Morris, T., Hiss, D.C., Boyom, F.F. and Mandal, C., Cytotoxicity potential of endophytic fungi extracts from Terminalia catappa against human cervical cancer cells. Journal of toxicology, 2020:1-9.

Rostinawati, T., Suryana, S., Fajrin, M. and Nugrahani, H., Aktivitas antibakteri ekstrak etanol daun kelakai (Stenochlaena palustris (Burm. F) Bedd) terhadap Salmonella typhi dan Staphylococcus aureus dengan metode difusi agar CLSI M02-A11. Pharmauho: Majalah Farmasi, Sains, Dan Kesehatan, 2018:3(1):1-5.

Kursia, S., Aksa, R. and Nolo, M.M., Potensi Antibakteri Isolat Jamur Endofit dari Daun Kelor (Moringa oleifera Lam.). Pharmauho, 2018:4(1):30-33.

Dhanti, K.R. and Sudarsono, T.A., Karakterisasi Morfologi Jamur dan Deteksi Aflatoksin pada Buah, Biji dan Sayuran dari Pasar Swalayan di Purwokerto. Jurnal Ilmiah Kesehatan, 2018:11(2).

Viogenta, P., Wathan, N., Sunardi, S. and Azizah, J., Profil FTIR dan GC/MS Ekstrak Jamur Endofit dari Akar Seluang Belum (Luvunga sarmentosa (Blume) Kurz.) Asal Kabupaten Tabalong Kalimantan Selatan. Jurnal Pharmascience, 2022:9(2): 344-354

Abna, I.M., Isolasi Dan Analisis Antimikroba Kapang Endofit Dari Tanaman Nangka (Artocarpus heterophyllus Lam). Jurnal Katalisator, 2021:6(2):146-163.

Bahri, S., Amelia, P., Hardini, A., Ramadhan, F. and Muhammad, A.A., Aktivitas antibakteri kapang endofit dari kulit batang tanaman Kayu Jawa (Lannea coromandelica (Hout.) Merr.) terhadap bakteri Streptococcus mutans dan Shigella dysenteriae. Jurnal Biotek Medisiana Indonesia, 2021:10(1):41-48.

Datta, F.U., Daki, A.N., Benu, I., Detha, A.I.R., Foeh, N.D. and Ndaong, N.A., Uji Aktivitas Antimikroba Bakteri Asam Laktat Cairan Rumen Terhadap Pertumbuhan Salmonella enteritidis, Bacillus cereus, Escherichia coli dan Staphylococcus aureus menggunakan metode difusi sumur agar. Jurnal Kajian Veteriner, 2019:66-85




DOI: https://doi.org/10.56711/jms.v4i1.1001

Refbacks

  • Saat ini tidak ada refbacks.


Indexed by:

     


ISSN: 2808-392X | e-ISSN: 2808-3911

Editor's Address:

3rd Floor Laboratory Faculty of Pharmacy,
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

Phone: +62 821 89555447
Fax: +62411425619
E-mail: jmic.sci@umi.ac.id