Isolation and Identification of Free Radical Scavenging Compound from Stem Bark Soursoup (Annona muricata L.) Extract

Authors

  • Mentarry Bafadal Faculty of Pharmacy, Universitas Halu Oleo, Indonesia
  • A Amaliah Dahlia Phytochemistry Division, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia
  • Ahmad Najib Pharmacognosy Division, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia

DOI:

https://doi.org/10.33096/jffi.v7i3.656

Keywords:

Acetogenin, Annona muricata L, Free antiradical, Isolation, Stem Bark

Abstract

Isolation and Identification of free radical active compound from stem bark soursoup (Annona muricata L.) extract. The aim of this research is to observe the chemical compound of stem bark the soursoup (A. muricata L.) which is active as a free radical. 550 gram of stem bark the soursoup (A. muricata L.) was by graduate extracted using n-heksan, ethyl acetat and ethanol solvents respectively. Extract result is the extraction, it was produced 2.4 gram n-heksan extract, 8.4 gram ethyl acetat extract, 2.1 gram ethanolic extract. The isolation of ethyl acetat extract was conducted using colom chromatography (TLC) method using eluent n-hexane : ethyl acetat (7:3) and it was produced four fractions. The third fraction was isolated using preparative thin layer chromatography with mob ile phase n-hexane : ethyl acetat (6:1) and got 2 bands. The isolate identified by UV-Vis spectroscopy, IR spectroscopy and analyzed by chemistry reaction. From the data result show that isolate is acetogenin.

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Published

2020-09-01

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Vol. 7 No. 3 (2020): JURNAL FITOFARMAKA INDONESIA (English Edition)

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