Antioxidant Activity of Bioproduced Endophytic Fusarium Sp. HSFP-3 from Sijangkang Plant (Hornstedtia scyphifera Var. fusiformis)

Penulis

  • Alfa Michael Gabriel Koraag Magister Farmasi Universitas Pancasila
  • Yunahara Farida Magister Farmasi Universitas Pancasila
  • Elsera Br. Tarigan Badan Riset Inovasi Nasional
  • Oscar Efendy Badan Riset Inovasi Nasional
  • Andria Agusta Badan Riset Inovasi Nasional
  • Partomuan Simanjuntak Magister Farmasi Universitas Pancasila Badan Riset Inovasi Nasional

DOI:

https://doi.org/10.33096/jffi.v11i3.1252

Kata Kunci:

Endophytic Fungi, Fusarium sp., Sijangkang, Antioxidant, DPPH

Abstrak

Endophytic mold Fusarium sp. HSFP-3 is one of the endophytic molds isolated from Sijangkang plants (Hornstedtia scyphyfera var. fusiformis). The purpose of this study is to conduct bioproduction, and test the antioxidant activity of bioproduced extracts and identify active fractions that have antioxidant activity. The research method started from the cultivation of endophytic mold HSFP-3 on PDB media for 21 days, the bioproduction results were extracted using ethyl acetate and acetone solvents. Antioxidant activity of HSFP-3 extract was determined by IC50 and AAI values, using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Isolation of antioxidant active fraction was carried out by column chromatography method using sephadex LH-20 stationary phase and silica. Isolation of antioxidant active compounds of HSFP-3 extract using bioautography guided assay approach with TLC-DPPH Bioautography method. The antioxidant test results of HSFP-3 extract has moderate activity with IC50 value 74,64 and AAI 0,844. Fraction 2.4 is an active fraction that has spot compounds with higher yellow color intensity compared to other fractions. Spot with Rf 0.35 in fraction 2.4 has a dark zone under UV 254 light. has a sea blue color under UV 366 light and a dark purple color after with Vanillin-Sulfate reagent

Biografi Penulis

  • Alfa Michael Gabriel Koraag, Magister Farmasi Universitas Pancasila
    Master of Pharmacy, Faculty of Pharmacy, Pancasila University Jakarta, Indonesia
  • Yunahara Farida, Magister Farmasi Universitas Pancasila
    Lecturer. Master of Pharmacy, Faculty of Pharmacy, Pancasila University Jakarta, Indonesia.
  • Elsera Br. Tarigan, Badan Riset Inovasi Nasional
    Research Center for Medicinal Raw Materials and Traditional Medicines (PR BBO-OT)
  • Oscar Efendy, Badan Riset Inovasi Nasional

    Research Center for Medicinal Raw Materials and Traditional Medicines (PR BBO-OT)

  • Andria Agusta, Badan Riset Inovasi Nasional
    Research Center for Medicinal Raw Materials and Traditional Medicines (PR BBO-OT)
  • Partomuan Simanjuntak, Magister Farmasi Universitas Pancasila Badan Riset Inovasi Nasional

    Lecturer. Master of Pharmacy, Faculty of Pharmacy, Pancasila University Jakarta, Indonesia.

    Research Center for Medicinal Raw Materials and Traditional Medicines (PR BBO-OT)

Referensi

Ardinis Arbin, Syamsurdi, n.d. Distribusi Hornstedtia Retz. (Zingiberaceae) di Sumatera. BioETI 76.

Bernatoniene, J., Kopustinskiene, D.M., 2018. The Role of Catechins in Cellular Responses to Oxidative Stress. Molecules. https://doi.org/10.3390/molecules23040965

Chen, X., Song, H., Zhou, S., Yuan, C., Li, J., 2023. Exploring separation patterns and mechanisms of proanthocyanidins in grape seeds and pomace with diverse molecular weights, compositions, and structures. Food Chem X 20, 101008. https://doi.org/https://doi.org/10.1016/j.fochx.2023.101008

Coskun, O., 2016. Separation Tecniques: Chromatography. North Clin Istanb. https://doi.org/10.14744/nci.2016.32757

Farida, Y., Qodriah, R., Nilesh, S., 2022. Quality Parameters And Determination Of Total Flavonoid Levels From The Highest Antioxidant Activity Of Ethanol 70% Extract Jackfruit Peel (Artocarpus Heterophyllus L.) By Maceration, Reflux, And Ultrasonic Methods. International Journal of Applied Pharmaceutics 14, 100–103. https://doi.org/10.22159/ijap.2022.v14s3.21

Fathoni, A., Hudiyono, S., Budianto, E., Cahyana, A.H., Agusta, A., 2021. Metabolite Detection and Antibacterial Activity of Fungal Endophytic Extracts Isolated from Brotowali (Tinospora crispa) Plants using TLC-Bioautography Assay, in: IOP Conference Series: Materials Science and Engineering. IOP Publishing Ltd. https://doi.org/10.1088/1757-899X/1011/1/012041

Foti, M.C., Daquino, C., Mackie, I.D., DiLabio, G.A., Ingold, K.U., 2008. Reaction of phenols with the 2,2-diphenyl-1-picrylhydrazyl radical. Kinetics and DFT calculations applied to determine ArO-H bond dissociation enthalpies and reaction mechanism. Journal of Organic Chemistry 73, 9270–9282. https://doi.org/10.1021/jo8016555

Mottaghipisheh, J., Iriti, M., 2020. Sephadex® LH-20, Isolation, and Purification of Flavonoids from Plant Species: A Comprehensive Review. Molecules. https://doi.org/10.3390/molecules25184146

Munir, E., Lutfia, A., Hartanto, A., Yurnaliza, Y., Lenny, S., 2022. Metabolites produced by endophytic fungi isolated from Hornstedtia scyphifera (J. Koenig) Steud. and their antioxidant activities. Agriculture and Natural Resources 56, 1249–1258. https://doi.org/10.34044/j.anres.2022.56.6.19

N. Eevers, M. Gielen, A. Sánchez-López, S. Jaspers, J. C. White, J. Vangronsveld, N. Weyens, 2015. Optimization of isolation and cultivation of bacterial endophytes through addition of plant extract to nutrient media. Microb Biotechnol 8, 707–715.

Praptiwi, Raunsai, M., Wulansari, D., Fathoni, A., Agusta, A., 2018. Antibacterial and antioxidant activities of endophytic fungi extracts of medicinal plants from Central Sulawesi. J Appl Pharm Sci 8, 069–074. https://doi.org/10.7324/JAPS.2018.8811

Praptiwi, Sulistiarini, D., Qodrie, E.N.P., Sahroni, D., 2021. Antibacterial activity, antioxidant potential, total phenolic and flavonoids of three plant species of Rubiaceae from Banggai Island, Indonesia. Biodiversitas 22, 2773–2778. https://doi.org/10.13057/biodiv/d220540

Salehi, B., Martorell, M., Arbiser, J.L., Sureda, A., Martins, N., Maurya, P.K., Sharifi-Rad, M., Kumar, P., Sharifi-Rad, J., 2018. Antioxidants: Positive or negative actors? Biomolecules. https://doi.org/10.3390/biom8040124

Santoni, A., Efdi, M., Suhada, A., 2019. Identifikasi Senyawa Metabolit Sekunder, Uji Antimikroba dan Antioksidan Ekstrak Akar Gantung Hornstedtia Scyphifera Var. Fusiformis Holttum (Sijangkang). Jurnal Riset Kimia 10, 94–98. https://doi.org/10.25077/jrk.v10i2.335

Shirly, K., 2019. Pemanfaatan Mikroba Endofit Dalam Bidang Farmasi. PT. ISFI Penerbitan, Jakarta Barat.

Simarmata, R., Naswita, S., Masaenah, E., Simanjuntak, P., 2022. Bioproduction of Antioxidant Chemical Compounds from Endophytic Bacteria of Red Betel Plants (Piper cf. fragile. Benth). Buletin Penelitian Tanaman Rempah dan Obat 33, 22. https://doi.org/10.21082/bullittro.v33n1.2022.22-30

Sy. Pakaya, M., Ain Thomas, N., Hasan, H., H. Hutuba, A., Mbae, G., 2023. Isolasi, Karakterisasi, dan Uji Antioksidan Fungi Endofit dari Tanaman Batang Kunyit (Curcuma domestica Val.,). Journal Syifa Sciences and Clinical Research 5. https://doi.org/10.37311/jsscr.v5i2.20341

Unduhan

Diterbitkan

2025-02-19

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Vol 11 No 3 (2024): JURNAL FITOFARMAKA INDONESIA (ENGLISH EDITION)

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