UJI AKTIVITAS EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM α-GLUKOSIDASE DENGAN MENGGUNAKAN ELISA READER
DOI:
https://doi.org/10.33096/jffi.v7i2.506Abstract
Kersen leaves (Muntingia calabura L) contain metabolites such as alkaloids, anthrax, polyphenols, tannins, saponins and are rich in flavonoid components such as flavones, flavonones, flavan and biflavan which have antidiabetic activity. According to a study conducted by Apriyanti (2016), the ethanol extract of kersen (Muntingia calabura L) at a dose of 250 mg / kg BB significantly reduced blood glucose levels in male wistar rats. In this study aimed at determining IC50 ethanol extract of Muntingia calabura L as an inhibitor of α-glucosidase enzymes using ELISA reader. The method was divided into 3 category is 1 (sample extract), 2 (blank) and 3 (akarbose). Each group added 25 µL α-glucosidase solution (0.25 units / mL then measured using ELISA reader 405 nm. The results showed that the ethanol extract of kersen (Muntingia calabura L) had activity as an inhibitor of α-glucosidase enzyme with IC50 34,197 µg / mL and can be categorized as active.References
Apriyanti, E 2016,’Efek Ekstrak Etanol Daun Kersen (Muntingia calabura L.) terhadap Penghambatan Peningkatan Kadar Gula Darah Pada Tikus Putih Jantan Galur Wistar’, Perpusnwu.web.id/karyailmiah/document
Arum, YP, Supartono, & Sudarmin 2012, ‘Isolasi dan Uji Daya Antimikroba Ekstrak Daun Kersen (Muntingia calabura)’, Journal UNNES, urnal MIPA, vol. 35, no. 2, pp 165-174, viewed 20 Desember 2016, http://journal.unnes.ac.id/sju/index.php/jm.
Chakrabarti R & Rajagopalan R 2002, Diabetes and Insulin Resistance associated disorders: Disease and the theraphy, IISC, Current science, Vol. 83, No. 12, viewed 18 Desember 2016, www.iisc.ernet.in/currscie/dec252002/1533.pdf .
Corwin & Elizabeth, J 2009, Buku Saku Patofisiologi edisi 3, EGC, Jakarta.
Departemen Kesehatan Republik Indonesia 2005,’Pharmaceutical Care untuk Penyakit Diabetes Melitus’, Jakarta: Direktorat Bina Farmasi Republik Indonesia.
Desmiaty, Y, Tambunan, RMarisi, & Pithaloka, LDyah 2014, ‘ Uji Aktivitas Penghambatan Enzim α-glukosidase serta Uji Mutu Ekstrak Etanol Batang Brotowali (Tinospora crispa L. Miers)’, Jurnal Ilmu Kefarmasian Indonesia, vol. 12, no. 2, pp 232-238, viewed 18 Desember 2016, http://jifi.ffup.org/wp-content/uploads/2015/11/232-237_yesi.pdf
Dewi, RT, et al 2007, ‘Inhibitory Effect of Koji Aspergillus terreus on α-glucosidase activity and postprandial hyperglycemia’, Pakistan Journal of Biological Science, vol. 10, no.18, pp 3131-3135, viewed 31 Desember 2016, s3.amazonaws.com/academia.edu.document.
Febrinda, AEarly, Astawan, M, Wesdiyati, T, & Yuliana, NDewi 2013, ‘Kapasitas Antioksidan dan Inhibitor Alpha Glukosidase Ekstrak Umbi Bawang Dayak’, J Teknol dan Industri Pangan, JPB, vol. 24, no. 2, pp 161-167, viewed 28 Desember 2016. http://journal.jpb.ac.id/index.php/jtip
Febriyani, V 2014,’ Uji Potensi Alfa Glukosidase dan Hipoglikemik Ekstrak Biji Mahoni (Swietenia Mahagoni Jacq) Sebaga Kandidat Obat Antidiabetes’, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Gunawan, S 2007, Farmakologi dan terapi Edisi 5, Fakultas Kedokteran UI, jakarta.
Hanani, E 2015, Analisis fitokimia, EGC, Jakarta
Hasanah, M, Andriani, N & Noprizon 2016,’Perbandingan Aktivitas Antioksidan Esktrak Etanol Daun Kersen (Muntingia calabura L.) Haasil Ekstraksi Maserasi dan Refluks’, SCIENTIA, viewed 12 Marete 2017, www.jurnalscientia.org/index.php/scientia/article/download /52/64
Henry, A, MT, S, & Yanuar, A 2002, ‘Analisis Spektrofotometri UV-Vis Pada Obat Influenza dengan Menggunakan Aplikasi Sistem Persamaan Linear, KOMMIT, viewed 27 Desember 2016, http://publication.gunadarma.ac.id/handle/123456789/3211
Hikmah, Z, 2015, ‘Uji Aktivitas Inhibitor Enzim Alfa-glukosidase Fraksi Etanol Daun Kenitu (Chrysophyllum cainito L.) berbagai Varian di Daerah Jember’, S.Si Skripsi, Fakultas Farmasi. Universitas Jember, Jember.
Hikmal, Alam, G, & Mufidah 2015, ‘Aktivitas Inhibitor Alpha Glukosidase Fungi Endofit MPD2 yang Diisolasi dari Tanaman Ongkea (Mezzetia parviflora Becc.)’, JST Kesehatan, vol. 5, no. 1, pp97-102, viewed 18 Desember 2016, http://pasca.unhas.ac.id/jurnal/files
Integrated Taxonomic Information System, 2016, Taxonomic Serial No.: 21503, viewed 23 Desember 2016, http://www.itis.gov/
Krishnaveni, M & Dhanalaksmi, R 2014, ‘Qualitative and Quantitative Study of Phytochemicals in Muntingia Calabura L. Leaf And Fruit’, WJPR, World Journal of Pharmaceutical Research, Vol. 3, No. 6, pp 1687-1696, viewed 18 Desember 2016, www.wjpr.net
Kimura et al 2003,’Two potent competitive Inhibitors Discriminating alpha glucosidase Family I From Family II’, ELSEVIER, viewed 14 Maret 2017, https://www.ncbi.nlm.nih.gov/pubmed/15063189.
Lebovitz, HE 1997,’Alpha Glukosidase Inhibitors-Endocrinology and Metabolism Clinics of North America’, Science direct, vol. 26, no. 3, pp 539-551, viewed 31 Desember 2016, www.sciencedirect.com/science.article/pii
Nair SS, Kavrekar V, & Mishra A 2013, ‘In vitro Studies on Alpha Amylase and Alpha Glucosidase Inhibitory Activities of Selected Plant Extracts, Pelagia Research library’, European Journal of Experimental Biology, Vol 3, No. 1, pp 128-132, viewed 18 Desember 2016, www.pelagiaresearchlibrary.com
Nurhasanah, N 2012, Isolasi Senyawa Antioksidan Ekstrak Metanol Daun Kersen (Muntingia calabura L), Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Jendral Achmad Yani.
Ophardt, CE 2003,’Enzyme Inhibitors’,Virtual chembook, viewed 31 Desember 2016, http://chemistry.elmhurst.edu/vchembook/573
Rais, IRidwan, et al 2013, ‘Penentuan Aktivitas Isolat Andrografolid Terhadap alpha amilase dan alpha Glukosidase menggunakan Metode Apostolidis and Mayur’, Traditional Medicine Journal, vol. 18, no. 3, pp 162-166, viewed 21 Desember 2016,
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