Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

Puteri Amelia; Rachma Ayunda; Saiful Bahri

doi:10.33096/jffi.v8i3.729

Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

Puteri Amelia, Rachma Ayunda, Saiful Bahri

Abstract

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

Keywords

Medinilla speciosa Blume; endophytic fungi; antibacterial activity; disk-diffusion method

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References

Aharwal, R. P., Kumar, S., Thakur, Y., Deshmukh, L., & Sandhu, S. S. (2018). Evaluation of Antibacterial Activity of Endophytic Fungi Aspergillus japonicus Isolated From Tridax procumbens L. Asian Journal of Pharmaceutical and Clinical Research, 11(9), 212. https://doi.org/10.22159/ajpcr.2018.v11i9.21564

Akanksha, B., Bhardwaj, A., & Agrawal, P. (2014). A review fungal endophytes: as a store house of bioactive compound. World Journal Of Pharmacy And Pharmaceutical Sciences, 3(9), 228–237.

Alsohaili, S. A., & Bani-Hasan, B. M. (2018). Morphological and molecular identification of fungi isolated from different environmental sources in the northern eastern Desert of Jordan. Jordan Journal of Biological Sciences, 11(3), 329–337.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005

Bhardwaj, A., Sharma, D., Jadon, N., & Agrawal, P. K. (2015). Antimicrobial and phytochemical screening of endophytic fungi isolated from spikes of Pinus roxburghii. Archives of Clinical Microbiology, 6(3), 1–9.

Christina, A., Christapher, V., & Bhore, S. (2013). Endophytic bacteria as a source of novel antibiotics: An overview. Pharmacognosy Reviews, 7(13), 11–16. https://doi.org/10.4103/0973-7847.112833

Gouda, S., Das, G., Sen, S. K., Shin, H. S., & Patra, J. K. (2016). Endophytes: A treasure house of bioactive compounds of medicinal importance. Frontiers in Microbiology, 7(SEP). https://doi.org/10.3389/fmicb.2016.01538

Hanum, A. S., Prihastanti, E., & Jumari. (2017). Ethnobotany of utilization, role, and philosopical meaning of parijoto (Medinilla, spp) on Mount Muria in Kudus Regency, Central Java. AIP Conference Proceedings, 1868(2017). https://doi.org/10.1063/1.4995210

Jayatilake, P. L., & Munasinghe, H. (2020). Antimicrobial Activity of Cultivable Endophytic and Rhizosphere Fungi Associated with “mile-a-Minute,” Mikania cordata (Asteraceae). BioMed Research International, https://doi.org/10.1155/2020/5292571

Kumala, S., DwiYuliani, K., & Simanjuntak, P. (2015). Antimicrobial activity of secondary metabolites produced by endophytic fungi isolated from stems of Jati tree (Tectonagrandis L.F). International Journal of Pharmaceutical Sciences and Research, 6(6), 2349–2353. https://doi.org/10.13040/IJPSR.0975-8232.6(6).2349-53

Ma, D., Zhu, J., Jiang, J., Zhao, Y., Li, B., Mu, W., & Liu, F. (2018). Evaluation of bioactivity and control efficacy of tetramycin against Corynespora cassiicola. Pesticide Biochemistry and Physiology, 152, 106–113. https://doi.org/10.1016/j.pestbp.2018.09.009

Mbilu, M., Wanyoike, W., Kangogo, M., Bii, C., Agnes, M., & Kihia, C. (2018). Isolation and Characterization of Endophytic Fungi from Medicinal Plant Warburgia ugandensis. Journal of Biology, Agricultue and Healthcare, 8(12), 57–66.

Navi, S. S., Bandyopadhyay, R., Hall, A. J., & Bramel -Cox, P. J. (1999). A Pictorial Guide for the Identification of Mold Fungi on Sorghum Grain Hall. In International Crops Research Institute for Semi Arid Tropics, 1999. (Issue 59).

Strobel, G., Daisy, B., Castillo, U., & Harper, J. (2004). Natural Products from Endophytic Microorganisms. Journal of Natural Products, 67(2), 257–268. https://doi.org/10.1021/np030397v

Sudirga, S. K. (2016). Isolasi Dan Identifikasi Jamur Colletotrichum Spp. Isolat Pcs Penyebab Penyakit Antraknosa Pada Buah Cabai Besar (Capsicum Annuum L.) Di Bali. Metamorfosa: Journal of Biological Sciences, 3(1), 23–30. https://doi.org/10.24843

Techaoei, S., Jirayuthcharoenkul, C., Jarmkom, K., Dumrongphuttidecha, T., & Khobjai, W. (2020). Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera. Saudi Journal of Biological Sciences, 27(11), 2883–2889. https://doi.org/10.1016/j.sjbs.2020.08.037

DOI: https://doi.org/10.33096/jffi.v8i3.729

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