Isolasi Dan Identifikasi Senyawa Kimia Monoterpen Dari Fraksi Etilasetat Daun Keji Beling (Strobilanthes crispa (L.) Blume)  Yang Mempunyai Daya Sitotoksik

Lilik Sulastri; Ristanti Mega Lestari; Partomuan Simanjuntak

doi:10.33096/jffi.v8i1.721

Isolasi Dan Identifikasi Senyawa Kimia Monoterpen Dari Fraksi Etilasetat Daun Keji Beling (Strobilanthes crispa (L.) Blume) Yang Mempunyai Daya Sitotoksik

Lilik Sulastri, Ristanti Mega Lestari, Partomuan Simanjuntak

Abstract

Isolation and identification one of chemical compound mono-terpene from Keji beling plant (local name) (Strobilanthes crispa) has been done. Extraction was carried out by maceration of the keji beling leaf powder with ethanol 96%, then partitioned with water-ethyl acetate. Ethylacetate extract was isolated and purified by a cytotoxic guide fractionation system with Brine Shrimp Lethality Test (BSLT) on column chromatography (SiO₂; n-hexane - ethylacetate = 20 : 1); and preparative Thin Layer Chromatography (TLC) (SiO₂; n-hexane - ethylaseate = 10 : 1) which gave one compound in oily form. Based on identification by infra red spectra and gas chromatography-mass spectra (GC-MS), the compound is a monoterpene D-limone which has a cytotoxic power of 73,11 ppm.

Keywords

D-limonene; monoterpene; keji Beling; Strobilanthus crispa (L.) Blume; cytotoxic

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References

Adibi, S. et al. (2017) ‘Induksi Tunas Keji Beling (Strobilanthes crispus L) Dengan Penambahan Kombinasi Naphtalen Acetic Acid (NAA) Dan 6-benzyl Amino Purin (BAP) Dalam Medium Cair’.

Buckley, J. P. (1966) ‘Pharmaceutical sciences (Np)’, Science, 151(3712), pp. 874–875. doi: 10.1126/science.151.3712.874.

Clarkson, C. et al. (2004) ‘In vitro antiplasmodial activity of medicinal plants native to or naturalised in South Africa’, Journal of Ethnopharmacology, 92(2–3), pp. 177–191. doi: 10.1016/j.jep.2004.02.011.

Dali, A. et al. (2017) ‘Uji Aktivitas Antioksidan Ekstrak Metanol Daun Pecah Beling (Strobilanthes crispus)’, Al-Kimia, 5(2), pp. 145–153.

Flowers, R. et al. (2006) ‘Best Practices in Microbiological Methodology.’, Aoac International, 12, p. 201.

Ghasemzadeh, A., Jaafar, H. Z. E. and Rahmat, A. (2015) ‘Phytochemical constituents and biological activities of different extracts of Strobilanthes crispus (L.) Bremek leaves grown in different locations of Malaysia’, BMC Complementary and Alternative Medicine. BMC Complementary and Alternative Medicine, 15(1), pp. 1–10. doi: 10.1186/s12906-015-0873-3.

Koay, Y. C. et al. (2013) ‘Chemical constituents and biological activities of strobilanthes crispus L’, Records of Natural Products, 7(1), pp. 59–64.

Koh, R. Y. et al. (2017) ‘Anticancer mechanisms of Strobilanthes crispa Blume hexane extract on liver and breast cancer cell lines’, Oncology Letters, 14(4), pp. 4957–4964. doi: 10.3892/ol.2017.6821.

Meyer, B. N., Ferrigni, N. R. and Putnam, J. E. (1982) ‘Brine shrimp: A convenient general bioassay for active plant constituents’, Planta Medica, 45(1), pp. 31–34. doi: 10.1055/s-2007-971236.

Nurraihana, H. and Norfarizan-Hanoon, N. A. (2013) ‘Phytochemistry, pharmacology and toxicology properties of Strobilanthes crispus’, International Food Research Journal, 20(5), pp. 2045–2056.

R. Hamidi, M., Jovanova, B. and Kadifkova Panovska, T. (2014) ‘Toxicological evaluation of the plant products using Brine Shrimp (Artemia salina L.) model’, Macedonian Pharmaceutical Bulletin, 60(01), pp. 9–18. doi: 10.33320/maced.pharm.bull.2014.60.01.002.

Rahmat, A. et al. (2006) ‘Anticarcinogenic Properties of Strobilanthes crispus Extracts and its Compounds in vitro’, pp. 47–49.

Setyawan, B. et al. (2016) ‘Pembuktian Ekstrak Daun Kejibeling Dalam Meningkatkan Sistem Imun Info Artikel Verification Of Kejibeling Leaf Extract In Improving The Immune System’, Kesehatan Masyarakat, 11(2).

Wijaya, H., Novitasari and Jubaidah, S. (2018) ‘Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambut Laut (Sonneratia caseolaris L. Engl)’, Jurnal Ilmiah Manuntung, 4(1), pp. 79–83.

DOI: https://doi.org/10.33096/jffi.v8i1.721

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