Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

Nurlinda Nurlinda; Virsa Handayani; Faradiba Abdul Rasyid

doi:10.33096/jffi.v8i3.712

Spectrophotometric Determination of Total Flavonoid Content in Biancaea Sappan (Caesalpinia sappan L.) Leaves

Nurlinda Nurlinda, Virsa Handayani, Faradiba Abdul Rasyid

Abstract

Abstract

Biancaea sappan (BS) is traditionally used to treat anticonvulsants, anti-inflammatory, antiproliferative, anticoagulant, antiviral, immunostimulant, antioxidant, and antimicrobial treatments. Flavonoids are found in Secang; flavonoids are secondary metabolites that have antioxidant activity. This study aims to identify the flavonoids using TLC and determination of flavonoids content in BS leaves. Initially, The methanol extract of BS was obtained by maceration with ethanol. The qualitative analysis of flavonoid was using TLC and visualization by sprayed with AlCl_3.The determination of total flavonoid content is conducted based on the AlCl3 method with total flavonoids expressed in QE (Quercetin equivalent) at the maximum wavelength of 431 nm. The research results showed that BS leaves contain flavonoids and the total flavonoid content of BS leaf extract is 1.0318 mg QE / g extract.

Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

Keywords

Keywords: Caesalpinia sappan; Flavonoid content, Spectrophotometric

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References

Ahmad, A,R, Juwita, Ratulangi, A, D dan Malik, A 2015, Penetapan Kadar Fenolik dan Flvonoid Total Ekstrak Metanol Buah dan Daun Patikala

(Etlingera elatior (Jack) R.M.SM), Pharmaceutical Science and Reseach, Vol. 2 No. 1

Dianasari, N 2009, ‘Uji Aktivitas Antibakteri Ekstrak Etanol Kayu BS(Caesalpinia sappan L.) Terhadap Staphylococcus dan Shygella dysentriae serta Bioautografinya’, S.Si Skripsi, Universitas

Muhammadiyah Surakarta,

Direktorat Jendral Pengawasan Obat dan Makanan 2000, ‘Parameter Standar Umum Ekstrak Tumbuhan Obat, Departemen Kesehatan Repoblik Indonesia, Jakarta.

Dyah N, A, Endang, K, Fahrauk, F 2014, ‘ Penetapan Kadar Flavonoid Metode AlCl3 pada Ekstrak Metanol Kulit buah Kakao (Theobroma cacao L.)’, Fakultas Farmasi Universitas Jendral Achmad Yani, Bandung

Gandjar, G. dan Rohman., 2009. Kimia Farmasi Analisis. Pustaka Pelajar : Yogyakarta.

Hanani, E 2015, Analisis Fitokimia, EGC, Jakarta, pp. 11

Harbone, J.B 1987, Metode Fitokimia, Penerbit ITB, Bandung

Heinrich, M, Bames, J, Gibbons, S, Williamson, E, M 2010, ‘ ‘Farmakognosi dan Fitoterapi’, Penerbit Buku Kedokteran, Jakarta

Ipandi I, Triyasmono L, Prayitno B 2016, Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan Ekstrak Etanol Daun Kajajahi (Leucosyke capitellata Wedd.), Jurnal Pharmascience, Vol 3 No. 1, Hal 93-100

Markham, K, R. 1988, ‘Cara Mengidentifikasi Flavonoid’, Padmawinata K, penerjemah, Terjemahan dari: Techniques of Flavonoid Identification.

Martinus, B, V, Verawati 2015, ‘ Penentuan Kadar Flavonoid Total dan Aktivitas Antioksidan dri ekstrak Daun Bandotan (Ageratium conyzoides L.), Sekolah Tinggi Farmasi Indonesia Perintis Padang, Scientia Vol. 5, No.

Miller, A, L 1996, ‘Antioxidant Flavonoids, Structure, Function and Clinical Usage,’ Alt, Med, Rev 1(2)

Mukhriani, Nonci, F, Y, Munawarah, S 2015, ‘ Analisis Kadar Flavonoid Total Pada Ekstrak Daun Sirsak (Annona muricata L.) dengan Metode

Spektrofotometri UV- Vis’, JK FIK UINAM Vol. 3, No. 2

Pertamawati, Nuralih, Fahrudin, F 2014, ‘ Ekstrak BSSebagai Bahan Diuretikum (Pecobaan terhadap Tikus Putih Jantan Galur Spraque Dawley’, Jurnal Biologi Vol. 7 No. 2

Saputra,A 2015 ‘Uji Aktivitas Antiinflamasi Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) Dengan Metode Stabilisasi Membran Sel Darah Merah Secara In Vitro’ S.Si Skripsi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri, Jakarta.

DOI: https://doi.org/10.33096/jffi.v8i3.712

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