AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI  ENDOFIT TANAMAN KUNYIT (Curcuma longa L.) TERHADAP PERTUMBUHAN  Staphylococcus aureus

Saskya Maulidya Astari; Ambar Rialita; Mahyarudin Mahyarudin

doi:10.33096/jffi.v8i2.644

AKTIVITAS ANTIBAKTERI ISOLAT BAKTERI ENDOFIT TANAMAN KUNYIT (Curcuma longa L.) TERHADAP PERTUMBUHAN Staphylococcus aureus

Saskya Maulidya Astari, Ambar Rialita, Mahyarudin Mahyarudin

Abstract

Staphylococcus aureus is a commensal bacterial of the body that can become pathogen at any time. Along with the increase of antibacterial resistant cases, it is necessary to explore the new potential metabolites as antibacterial. Endophytic bacteria that live on turmeric (Curcuma longa L.) are thought to be able to produce secondary metabolites that are similar and even more varied than their host plants.To determine the antibacterial effect of secondary metabolite compounds of turmeric (Curcuma longa L.) endophytic bacteria on Staphylococcus aureus. Endophytic bacterial isolates were subcultured on NA media with a streak plate method. Antibacterial activity test useds disk diffusion method. Potential endophytic bacterial isolates were characterized based on colony morphology, cell morphology and biochemical properties and secondary metabolite compounds produced are identified with a ciulei method. A total of 17 endophytic bacterial isolates were successfully subcultured. All endophytic bacterial isolates had antibacterial activity range from 7.4-13.76 mm. One of the most potential isolates was M8 isolate which had similarity with a genus Pseudomonas. The secondary metabolites that was produced bt M8 isolates were alkaloids, terpenoids and saponins. The most potential endophytic bacteria as antibacterial belong to the genus Pseudomonas and had alkaloids, terpenoids and saponins.

Keywords

Endophytic bacteria; Tumeric; Staphylococcus aureus

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References

Adityawarman (2017) ‘Isolasi, identifikasi dan aktivitas antibakteri bakteri endofit daun pegagan (Centella asiatica L.) terhadap Escherichia coli’, SKRIPSI.

Ahmed, N. et al. (2008) ‘Antibiotic Bushrin’, US Patent Publication No US 2008/0090900 A1.

Almeida, C. et al. (2017) ‘Time-dependent production of the bioactive peptides endolides A and B and the polyketide mariline A from the Sponge-derived Fungus Stachylidium bicolor 293K04’, Fermentation, 3(45), pp. 1–12.

Chakraborty, A., Kundu, S. and Ghosh, B. (2019) Endophytism in Zingiberaceae: Elucidation of beneficial impact. Springer Nature Switzerland.

Chen CJ and Huang YC (2014) ‘New epidemiology of Staphylococcus aureus infection in Asia’, Clinical Microbiology Infection, 20(7), pp. 605–606.

Dewi, A. (2013) ‘Isolasi, identifikasi dan uji sensitivitas Staphylococcus aureus terhadap Amoxillin dari sampel susu kambing, peranakan ettawa (PE) penderita mastitis di wilayah Grimulyo, Kulomprogo, Yogyakarta’, Jurnal Sain Veteriner, 31(2), pp. 138–150.

Erikawati, D., Santosaningsih, D. and Santoso, S. (2016) ‘Tingginya prevalensi MRSA pada isolat klinik periode 2010-2014 di RSUD Dr. Saiful Anwar Malang, Indonesia’, Jurnal Kedokteran Brawijaya, 29(2), pp. 149–156.

Halim, Jasril and Saryono (2014) ‘Optimalisasi produksi senyawa metabolit sekunder dari Pseudomonas sp. endofit tanaman dahlia (Dahlia variabilis)’, Ind.Che.Acta, 5(1), pp. 8–14.

Haryati, N., Saleh, C. and Erwin (2015) ‘Uji toksisitas dan aktivitas antibakteri ekstrak daun merah tanaman pucuk merah (Syzygium myrtifolium walp.) terhadap bakteri Staphylococcus aureus dan Escherichia coli’, Jurnal Kimia Mulawarman, 13(1), pp. 35–40.

Holt, J. et al. (2000) Bergey’s of determinative bacteriology. 9th edn. USA: A Wolters Kluwer Company Philadelphia.

Hudzicki, J. (2009) Kirby-Bauer Disk Diffysion Susceptibility Test Protocol, American Society for Microbiology.

Husna, C. (2018) ‘Peran protein adhesi ekstraselular dalam patogenitas bakteri Staphylococcus aureus’, Jurnal Averrous, 4(2), pp. 1–12.

Indrawati, I., Rossiana, N. and Diresna, D. (2018) ‘Bioprospecting of bacterial endophytes from Curcuma aeruginosa, Curcuma xanthorrhiza and Curcuma zedoariaas antibacterial against pathogenic bacteria’, IOP Conf. Series: Earth and Environmental Science 197, pp. 1–7.

Jayatilake, G. et al. (1996) ‘Metabolites from an Antarctic sponge-associated bacterium, Pseudomonas aeruginosa’, Journal of Natural Products, 59(3), pp. 293–296.

Kemenkes RI (2011) ‘Peraturan Menteri Kesehatan Republik Indonesia Nomor 2406/Menkes/PER/XII/2011 tentang Pedoman umum penggunaan antibiotik’.

Kumar, A. et al. (2016) ‘Isolation and characterization of bacterial endophytes of Curcuma longa L.’, Biotech, 6(60), pp. 1–8.

Kurniawati AFS, Satyabakti P, A. N. (2015) ‘Perbedaan risiko multidrug resistance organism (MDROS) menurut faktor risiko dan kepatuhan hand hygiene’, Jurnal Berkala Epidemiologi, 3(3), pp. 277–289.

Liana P (2014) ‘Gambaran kuman Methicillin-Resistant Staphylococcus aureus (MRSA) di Laboratorium Mikrobiologi Departemen Patologi Klinik Rumah Sakit Dr. Cipto Mangunkusumo (RSCM) periode januari-desember 2010’, MKS, 46(3), pp. 171–175.

Machmud, M. (2001) ‘Teknik Penyimpanan dan Pemeliharaan Mikroba’, Balai Penelitian Bioteknologi Tanaman Pangan, 4(1), pp. 24–32.

Muharni et al. (2014) ‘Antibacterial and antioxidant activity testing of pyranon derivated compound from endophytic fungi penicillium sp of kunyit putih ( Curcuma zedoaria ( Berg ) Roscoe )’, Trad. Med. J., 19(3), pp. 107–112.

Omejate, G. et al. (2014) ‘Mechanisms of antimicrobial actions of phytochemicals against enteric pathogens – A Review’, Journal of Pharmaceutical, Chemical and Biological Sciences, 2(2), pp. 77–85.

Pinu, F. and Boas, S. (2017) ‘Extracellular microbial metabolomics: The state of the art’, Metabolites, 7(43), pp. 1–15.

Pratiwi, R. et al. (2011) ‘Identification and characterization of three endophytic bacteria from Neesia altissima(Malvaceae) antagonistic to diarrhea-causing bacteria’, Malaysian Journal of Microbiology, 12(4), pp. 300–307.

Rahmi, Y. et al. (2015) ‘Identifikasi bakteri Staphylococcus aureus pada preputium dan vagina kuda (Equus caballus)’, Jurnal Medika Veterinaria, 9(2), pp. 154–158.

Rizaldi, H. (2018) ‘Karakterisasi bakteri Gram positif endofit tanaman kunyit (Curcuma longa) yang memiliki kemampuan quorom quenching’, SKRIPSI.

Ryu, S. et al. (2014) ‘Colonization and infection of the skin by S. aureus: immune system evasion and the response to cationic antimicrobial peptides’, International Journal of Molecular Sciences, 15, pp. 8754–8772.

Singh, M. et al. (2017) ‘Endophytic bacteria: a new source of bioactive compounds’, Biotech, 7(315), pp. 1–14.

Sulistiyani, Ardyati, T. and Winarsih, S. (2016) ‘Antimicrobial and antioxidant activity of endophyte bacteria associated with Curcuma longa Rhizome’, J.Exp. Life Sci., 6(1), pp. 45–51.

Sulistiyani, R. et al. (2014) ‘Population and diversity of endophytic bacteria associated with medicinal plant Curcuma zedoaria’, Microbiology Indonesia, 8(2), pp. 65–72.

Takajian S (2014) ‘New epidemiology of Staphylococcus aureus infection in the middle east’, Clinical Microbiology and Infection (CMI), 20(7), pp. 624–628.

Tchaou, W. and dkk (1996) ‘Inhibition of pure cultures of oral bacteria by root canal filling materials’, Pediatric Dentistry, 18(7), pp. 444–449.

Tong, S. Y. C. et al. (2015) ‘Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management’, Clinical Microbiology Reviews, 28(3), pp. 603–661. doi: 10.1128/CMR.00134-14.

Triana, O., Sarjono, P. and Mulyani, N. (2017) ‘Isolasi bakteri endofit pada rimpang jahe merah (Zingiber officinale Linn. Var Rubrum) penghasil senyawa antioksidan’, Jurnal Kimia Sains dan Aplikasi, 20(1), pp. 25–29.

Uzair, B. et al. (2008) ‘The isolation, puriWcation and biological activity of a novel antibacterial compound produced by Pseudomonas stutzeri’, FEMS Microbiol Lett, 279, pp. 243–250.

Widowati Tiwit, D. (2016) ‘Isolasi dan Identifikasi Kapang Endofit dari Tanaman Kunyit sebagai Penghasil Antioksidan’, Biopropal Industri, 7(1), pp. 9–16.

Yuanita, M. (2018) ‘Eksplorasi bakteri gram negatif endofit tanaman kunyit (Curcuma longa L.) yang memiliki kemampuan quorum quenching’, SKRIPSI.

Yuliati (2016) ‘Uji efektivitas ekstrak kunyit sebagai antibakteri dalam pertumbuhan Bacillus sp dan Shigella dysentriae secara in vitro’, Jurnal Profesi Medika, 10(1), pp. 25–32.

Zulkifli, L. et al. (2016) ‘Isolasi bakteri endofit dari sea grass yang tumbuh di kawasan pantai pulau lombok dan potensinya sebagai sumber antimokroba terhadap bakteri patogen’, Jurnal Biologi Tropis, 16(2), pp. 80–93.

DOI: https://doi.org/10.33096/jffi.v8i2.644

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