Aktivitas Antiinflamasi Ekstrak Etanol Dan Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Menggunakan Metode Spektrofometri UV-Vis

Asriani Suhaenah; Siska Nuryanti; Zainal Abidin; Nur Evatiara

doi:10.33096/jffi.v11i2.1288

Aktivitas Antiinflamasi Ekstrak Etanol Dan Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Menggunakan Metode Spektrofometri UV-Vis

Asriani Suhaenah, Siska Nuryanti, Zainal Abidin, Nur Evatiara

Abstract

Ficus is a species that is rich in polyphenolic compounds, such as flavonoids. These compounds are strong antioxidants that can aid in the prevention and treatment of various diseases caused by oxidative stress. They also protect cell structures, have anti-inflammatory properties, and can act as antibiotics. The aim of this study was to evaluate the anti-inflammatory properties of the ethanol extract and ethyl acetate fraction of rubber kebo (Ficus elastica) leaves using the Bovine Serum Albumin (BSA) protein denaturation method. The measurements were taken on a Uv-Vis spectrophotometer at a wavelength of 660 nm. The study consisted of four experimental groups: a negative control, a positive control of diclofenac sodium, and test groups of ethanol extract and ethyl acetate fraction. The study revealed that the positive control (IC₅₀) had an anti-inflammatory value of 9.2 μg/mL. Additionally, the ethanol extracts and ethyl acetate fractions of rubber kebo leaves (Ficus elastica) demonstrated anti-inflammatory activity with IC₅₀ values of 31.24 μg/mL and 37.89 μg/mL, respectively. These findings suggest that the ethanol extracts and ethyl acetate fractions of rubber kebo leaves possess anti-inflammatory properties.

Keywords

anti-inflammatory; bovine serum albumin; ethanol extract of rubber kebo leaf; ethyl acetate fraction of rubber kebo leaf

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References

Abidin, Z., Putri, U.A. and Widiastuti, H. (2020) ‘Potensi Anti-inflamasi Fraksi Etil Asetat Ranting Patah Tulang (Euphorbia tirucalli L.) dengan Uji Penghambatan Denaturasi Protein’, ad-Dawaa’ Journal of Pharmaceutical Sciences, 2(2), pp. 49–54. Available at: https://doi.org/10.24252/djps.v2i2.11549.

Aditya, M.R.T. et al. (2015) ‘Potensi Antiinflamasi Buah Manggis( Garcinia mangostana ) Terhadap Denaturasi Protein In Vitro’, pp. 149–156.

Bashori, Y.M. (2008) Efek Antiinflamasi Ekstrak Etanol Daun Belimbing Wuluh ( Averrhoa bilimbi Linn .) Pada Tikus Putih Jantan Yusuf Muamar Bashori Fakultas Farmasi. Universitas Muhammadiyah Surakarta.

Becker, D.E. (2013) ‘Basic and clinical pharmacology of Glucocorticosteroids’, Anesthesia Progress, 60(1), pp. 25–32. Available at: https://doi.org/10.2344/0003-3006-60.1.25.

Burhannuddin, B. and Karta, I.W. (2023) ‘Uji Aktivitas Antiinflamasi Teh Cang Salak Secara In Vitro Dengan Metode Stabilisasi Membran Human Red Blood Cell’, Jurnal Fitofarmaka Indonesia, 10(2), pp. 39–46. Available at: https://doi.org/10.33096/jffi.v10i2.903.

Can-aké, R. et al. (2004) ‘Bioactive Terpenoids from Roots and Leaves of Jatropha gaumeri’, pp. 11–14.

Czub, M.P. et al. (2021) ‘HHS Public Access Author manuscript Drugs in Mammalian Blood Plasma’, J Med Chem, 63(13), pp. 6847–6862. Available at: https://doi.org/10.1021/acs.jmedchem.0c00225.SMILES.

Dwiyani, R. (2013) Mengenal Tanaman Pelindung Di Sekitar Kita. Denpasar : Udayana University Press.

Handayani, S., Kurniawati, I. and Rasyid, F.A. (2020) ‘Uji Aktivitas Antioksidan Ekstrak Daun Karet Kebo (Ficus Elastica) dengan Metode Peredaman Radikal Bebas Dpph (1,1-Diphenyl-2-Picrylhydrazil)’, Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 6(1), pp. 141–150. Available at: https://doi.org/10.22487/j24428744.2020.v6.i1.15022.

Hermawati, E., Abdillah, S. and Rahmat, D. (2021) ‘Efek Antiinflamasi Nanopartikel Kitosan-Ekstrak Dan In Vivo In Vitro And In Vivo Anti-Inflammatory Effects Of Chitosan Nanoparticles-Dried Extract Of Sea Cucumber ( Stichopus variegatus )’, pp. 115–132.

Hidayati, S. et al. (2022) ‘Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak etanol Daun Mangga Arumanis (Mangifera indica L.) ‘, Jurnal Sains dan Kesehatan, 4(5), pp. 488–494.

Kumar, V. et al. (2012) ‘Evaluation of anti-inflammatory potential of leaf extracts of Skimmia anquetilia.’, Asian Pacific journal of tropical biomedicine, 2(8), pp. 627–630. Available at: https://doi.org/10.1016/S2221-1691(12)60109-9.

Marrassini, C., Peralta, I. and Anesini, C. (2018) ‘Comparative study of the polyphenol content ‑ related anti ‑ inflammatory and antioxidant activities of two Urera aurantiaca specimens from different geographical areas’, Chinese Medicine, pp. 1–12. Available at: https://doi.org/10.1186/s13020-018-0181-1.

Novika, D.S., Ahsanunnisa, R. and Yani, F. (2021) ‘Stannum : Jurnal Sains dan Terapan Kimia Anti-Inflammatory Activity of Ethanol Extract of Starfruit Leaves ( Averrhoa bilimbi L .) Against Inhibition of Protein Denaturation Uji Aktivitas Antiinflamasi Ekstrak Etanol Daun Belimbing Wuluh ( Averrhoa bilimb’, 3(1), pp. 16–22. Available at: https://doi.org/10.33019/jstk.v3i1.2117.

Roslyana, I., Rahayu, T. and Widiastuti, L. (2021) ‘Pengaruh Macam Media dan PGPR Terhadap Keberhasilan Stek Tanaman Karet Kebo (Ficus Elastica)’, AGRISAINTIFIKA: Jurnal Ilmu-Ilmu Pertanian, 5(2), p. 176. Available at: https://doi.org/10.32585/ags.v5i2.1933.

Suhaenah, A., Pratama, M. and Amir, A.H.W. (2021) ‘Penetapan Kadar Flavonoid Fraksi Etil Asetat Daun Karet Kebo (Ficus elastica) Dengan Metode Spektrofotometri Uv-Vis’, 13(1), pp. 48–54.

Tukiran et al. (2023) ‘Kadar Total Flavonoid dan Aktivitas Antiinflamasi Kombinasi Ekstrak Etanol Secang ( Caesalpinia sappan L .) dan Jahe Merah ( Zingiber officinale Roxb .) Terhadap Penghambatan Denaturasi Protein Bovien Serum Albumin’, 6573, pp. 31–39. Available at: https://doi.org/10.17977/um0260v7i12023p031.

Zukhri, S. and Nurhaini, R. (2019) ‘Uji Aktivitas Antibakteri Ekstrak Etanol Daun Karet Kerbau (Ficus elastica Roxb. Ex Hornem.) Terhadap Bakteri Staphylococcus aureus’, Motorik, Jurnal Ilmu Kesehatan, 14(01), pp. 58–70.

DOI: https://doi.org/10.33096/jffi.v11i2.1288

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