THE EXTRACT STANDARDIZATION TEST AND PHYSICAL PROPERTIES EVALUATION OF UNCARIA GAMBIER (HUNTER) ROXB. EXTRACT CATECHINE CAPSULES

dina melia oktavilantika; Dona Suzana; Ditiya Himawati; Hendri Dwi Putra

doi:10.33096/jffi.v11i3.1048

THE EXTRACT STANDARDIZATION TEST AND PHYSICAL PROPERTIES EVALUATION OF UNCARIA GAMBIER (HUNTER) ROXB. EXTRACT CATECHINE CAPSULES

dina melia oktavilantika, Dona Suzana, Ditiya Himawati, Hendri Dwi Putra

Abstract

Society does not really know that the catechin extract contained in uncaria gambir roxb can be used as an anti-inflammatory and antioxidant. The catechins in gambier plant are very complex. They are composed of extracts of catechins (C), epicatechins (EC), epicatechin gallate (ECG), epigalocatechin (EGC), epigalocatechin gallate (EGCG), and galocatechin (GC). Thus, the use of this medicinal plants with anti-inflammatory and antioxidant properties needs to be developed as an alternative treatment. In addition, this species of plant has also important effectiveness and relatively smaller side effects. In an effort to increase efficiency and maximize the therapeutic effects of gambier as an anti-inflammatory and antioxidant, the authors need to develop an innovate pharmaceutical product from this plant. One of the selected pharmaceutical preparations is the capsule since it can conceal the taste and the unpleasant smell; besides, it is also easy to consume. The maceration method was carried out in the catechin extraction process. After obtaining pure catechin extracts, the authors tested catechin levels using the HPLC method and antioxidant levels using the DPPH method. Next, the authors performed extract standardization tests, and evaluated the physical properties of the capsule preparations. The test results showed catechin levels of 98.697% and IC50 antioxidant levels of 8.54 µg/dl. The results of the standardized extract and capsule evaluation tests indicated that they met the applicable requirements.

Keywords

catechins, extract standardization test, capsule physical evaluation, assay, antioxidant test

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References

Depkes RI. (2000). Parameter standar umum ekstrak tumbuhan obat : Jakarta Departemen Kesehatan Republik Indonesia. Edisi IV.

Depkes RI. Farmakope Indonesia. Edisi IV. Jakarta : Departemen Kesehatan RI, 1995.

Hasibuan, A. S., Edrianto, V., & Purba, N. (2021). SKRINING FITOKIMIA EKSTRAK ETANOL UMBI BAWANG MERAH (Allium Cepa L.). Jurnal Pengmas Kestra (Jpk), 1(1), 80–84. Https://Doi.Org/10.35451/Jpk.V1i1.732

Kementrian Kesehatan R.I, Farmakope Herbal Indonesia, Edisi 1,Dirjen Pelayanan Farmasi dan alat Kesehatan, Jakarta, 2008

Tandi, J., Melinda, B., Purwantari, A., & Widodo, A. (2020). Analisis Kualitatif Dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus Esculentus L. Moench) Dengan Metode Spektrofotometri UV-Vis. KOVALEN: Jurnal Riset Kimia, 6(1), 74–80. Https://Doi.Org/10.22487/Kovalen.2020.V6.I1.15044

Santi I, Putra B, Wahyuni S. Uji Efek ekstrak etanol daun gambier (Cyclea barbata Miers) sebagai anti inflamasi pada tikus putih yang diinduksi karagen. As- Syifaa.

Voight R. Buku Pelajaran Teknologi Farmasi. Edisi V. Soendani Noerno Soewandi , translator. Yogyakarta: Universitas Gadjah Mada Press, 1994.2017;09(01):58-66.

Wallach MD. (2007). Interpretation of Diagnostic Test, Eight Edition. Philadelphia: Lippincott Williams and Wilkins

Aditya, M., & Ariyanti, P. R. (2016). Manfaat Gambier ( Uncaria gambier Roxb ) sebagai Antioksidan Benefits of Gambier ( Uncaria gambier Roxb ) as Antioxidant. Majority, 5(September),129–133. http://juke.kedokteran.unila.ac.id/index.php/majority/article/view File/1049/844

Desta Donna Putri Damanik, Surbakti, N., & Hasibuan, R. (2014). Ekstraksi Katekin ( Uncaria Gambier roxb ) dengan Metode Maserasi. Jurnal Teknik Kimia USU, 3(2), 10–14. https://doi.org/10.1007/978-1-4419-0823-0

Pérez-Jiménez, J. & Saura-Calixto, F. (2006) Effect of Solvent and Certain Food Constituents on Different Antioxidant Capacity Assays. Food Research International. [Online] 39 (7), 791–800. Available from: doi:10.1016/j.foodres.2006.02.003.

Sultana, B., Anwar, F. & Ashraf, M. (2009) Effect of Extraction Solvent/Technique on the Antioxidant Activity of Selected Medicinal Plant Extracts. Molecules. [Online] 14 (6), 2167–2180. Available from: doi:10.3390/molecules14062167

Watanabe, M. (1998) Catechins as Antioxidants from Buckwheat ( Fagopyrum esculentum Moench) Groats. Journal of Agricultural and Food Chemistry. [Online] 46 (3), 839–845. Available from: doi:10.1021/jf9707546.

Widyasanti, A., Rohdiana, D. & Ekatama, N. (2016) Aktivitas antioksidan ekstrak teh putih (Camellia sinensis) dengan metode dpph (2, 2 difenil-1- pikrilhidrazil). Journal of Materials Processing Technology. 1 (1), 1–9.

DOI: https://doi.org/10.33096/jffi.v11i3.1048

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