AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL BATANG WOLE WOE TERHADAP MIKROBA PATOGEN MENGGUNAKAN DIFUSI AGAR

Fitriana Fitriana, Sitti Amirah, Safriani Rahman, Sukmawati Sukmawati

Abstract


Infectious diseases can be caused by the entry of microorganisms such as bacteria, fungi, parasites, and viruses that can cause diseases in humans. One of the plants used empirically by the Weda community in Central Halmahera is the stem part of the wole woe plant. The aim of this study was to determine the antimicrobial activity of the ethanol extract of wole woe stems against pathogenic microbes such as Propionibacterium acnes, Escherichia coli, Salmonella thypi, Shigella dysentriae, Vibrio cholerae, and Candida albicans. This study used the agar diffusion method with various concentrations of 2.5%, 5%, and 10%, and positive controls for bacteria with chloramphenicol, positive controls for fungi with nystatin, and DMSO as a negative control. Based on the average diameter of the inhibition zone obtained, the test microbes were classified into the medium-strong category because they had an inhibition zone diameter ranging from 9 mm to 13 mm. The positive control for chloramphenicol for bacteria was classified into the very strong category because it had an inhibition zone diameter of >20 mm, while the positive control for nystatin for fungi was classified into the strong category because it had an inhibition zone diameter of >10 mm. Based on these tests, it can be concluded that the ethanol extract of wole woe stems has antimicrobial activity

Keywords


Antimicrobial; Pathogenic Microbes; Wole Woe Stem

References


Mutsaqof AAN, Wiharto, Suryani E. Sistem Pakar Untuk Mendiagnosis Penyakit Infeksi Menggunakan Forward Chaining. ITSMART: Jurnal Teknologi dan Informasi. 2016; 4(1):43–47

Juariah S, Suryanto D, Jamilah I. Aktifitas Anti Bakteri Spesies Asterias Forbesii Terhadap Beberapa Jenis Bakteri Patogen. Berkala Perikanan Terubuk. 2016; 42(2):37–50

Fitriana, Amirah S, Rahman S. Aktivitas Antimikroba Ekstrak Etanol Batang Wole Woe Asal Halmahera Tengah Terhadap Bakteri Gram Positif Staphylococcus epidermidis dan Staphylococcus aureus. As-Syifaa Jurnal Farmasi. 2022; 14(1):57–65

Rahman S, Kosman R, Amirah S. Uji Efek Epitelisasi Ekstrak Batang Wole Woe Asal Kabupaten Halmahera Tengah Terhadap Penyembuhan Luka Bakar Pada Tikus. As-Syifaa Jurnal Farmasi. 2022; 14(1):48–56

Fitriana, Amirah S, Rahman S, Putra B. Potensi Antibakteri Ekstrak Etanol Batang Wole Woe Asal Halmaherah Tengah Terhadap Bakteri Pseudomonas aeruginosa dan Bacillus subtilis Menggunakan Metode Difusi Agar. As-Syifaa Jurnal Farmasi. 2022; 14(2):155–161

Handayani V, Rahman S, Amaliah ANA. Uji Toksisitas Ekstrak Etanol Batang Kayu Wole Woe Terhadap Larva Artemia salina Leach Menggunakan Metode Brine Shrimp Lethality Test (BSLT). As-Syifaa Jurnal Farmasi. 2022; 14(2):131–138

Sineke FU, Suryanto E, Sudewi S. Penentuan Kandungan Fenolik dan Sun Protection Factor (SPF) Dari Ekstrak Etanol Dari Beberapa Tongkol Jagung (Zea mays L.). PHARMACON. 2016; 5(1):275–283

Asfi D. Uji Efektivitas Losio Ekstrak Daun Kembang Sore (Abutilon indicum L) Terhadap Pertumbuhan Staphylococcus aureus. Jurnal Kesehatan Yamasi Makassar. 2021; 5(1):33–38

Nuryanti S. Aktivitas Antifungi Sari Daun Pepaya (Carica papaya L.) Terhadap Candida albicans. As-Syifaa Jurnal Farmasi. 2017; 9(2):137–145

Maryam St, Juniasti S, Kosman R. Uji Aktivitas Antibakteri Ekstrak Etanol Buah Belimbing Wuluh (Averrhoa bilimbi L.) Asal Kota Watampone. As-Syifaa Jurnal Farmasi. 2015; 7(1):60–69

Hasanuddin ARP, Salnus S. Uji Bioaktivitas Minyak Cengkeh (Syzygium aromaticum) Terhadap Pertumbuhan Bakteri Streptococcus mutans Penyebab Karier Gigi. BIOMA: Jurnal Biologi Makassar. 2020; 5(2):241–250

Kusumawati E, Saputri WR, Supriningrum R. Uji Aktivitas Antifungi Ekstrak Etanol Akar KB (Coptosapelta tomentosa Valeton Ex K. Heyne) Terhadap Candida albicans Secara in Vitro. Polhasains: jurnal sains dan terapan Politeknik Hasnur. 2020; 8(01):1–9

Mulyadi Moh, Wuryanti W, Sarjono PR. Konsentrasi Hambat Minimum (KHM) Kadar Sampel Alang-Alang (Imperata cylindrica) dalam Etanol Melalui Metode Difusi Cakram. Jurnal Kimia Sains dan Aplikasi. 2017; 20(3):130–135

Octaviani M, Fadhli H, Yuneistya E. Uji Aktivitas Antimikroba Ekstrak Etanol Kulit Bawang Merah (Allium cepa L.) dengan Metode Difusi Cakram. Pharmaceutical Sciences and Research. 2019; 6(1):62–68

Katzung BG. Farmakologi Dasar Dan Klinik (Ed 4). Jakarta: EGC. 1994

Utomo SB, Fujiyanti M, Lestari WP, Mulyani S. Antibacterial Activity Test of the C-4-Methoxyphenylcalix [4] Resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria. JKPK (Jurnal Kimia dan Pendidikan Kimia). 2018; 3(3):201–209

Fauziah GF. Perbedaan Potensi Antijamur Ekstrak Etanolik Kulit Manggis (Garcinia mangostana L.) dengan Nistatin Terhadap Candida albicans In Vitro (Skripsi). Yogyakarta: Fakultas Kedokteran Gigi. 2014

Pelczar MJ, Chan ECS. Dasar-Dasar Mikrobiologi. Jakarta: Universitas Indonesia Press. 1988




DOI: https://doi.org/10.56711/jifa.v15i1.967

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Faculty of Pharmacy, Univeristas Muslim Indonesia
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