EFEK ANTINEFROTOKSISITAS EKSTRAK ETANOL DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb.) DENGAN PARAMETER KADAR UREUM TIKUS PUTIH

Sukmawati Sukmawati, Rahmawati Rahmawati, Kenanga Isyanda A

Abstract


Nephrotoxicity caused by chemical compounds such as drugs. This condition can cause an increase in urea levels. Antioxidants used to inhibit nephrotoxicity by protect and repair cells. One of the plants as antioxidant is the pandan wangi leaf (Pandanus amaryllifolius Roxb.) which contains phenolic to absorb and neutralize free radicals or decompose peroxides. This study aimed to examine the antinephrotoxicity effect of the ethanolic extract of pandan wangi leaf (Pandanus amaryllifolius Roxb.) on kidney damage with parameters of urea level in rats. Eighteen male Wistar rats were divided into 6 groups. Group 1 as a naive group, group 2 as a negative control by Gentamicin, group 3 as a positive control by amino acid supplement, and groups 4, 5, and 6 were given ethanol extract of pandan wangi leaves at a dose by 125 mg/kgBW, 250 mg/kgBW and 500 mg/kgBW respectively. Nephrotoxicity condition was induced by Gentamicin 100 mg/kgBW (ip) for seven days. The blood samples were taken and measured the serum creatinine levels on days 0, 8th and 21st by Human Analyzer. The results study showed that ethanol extract of pandan wangi leaves at 125 mg/kgBW have the effect as antinephrotoxicity based on decreased ureum levels

Keywords


Gentamicin; nephrotoxicity; pandan wangi; Pandanus amaryllifolius roxb; ureum

References


Guyton AC, Hall JE. Fisiologi Kedokteran. Jakarta: EGC, 2007.

Goodman SL, Gilman A. Farmakologi Dasar Terapi. Jakarta: EGC, 2008.

Chandrasoma P, Taylor R. Ringkasan Patologi Anatomi. Jakarta: EGC, 2006.

Katzung BG. Farmakologi Dasar dan Klinik volume 2. Jakarta: EGC, 2013.

Kumar V, Abbas A, Aster. Robbins basic pathology 10th Edition. Philadelphia: Elsevier, 2018.

Sharma RK, Rajani GP, Sharma V, Komala N. Effect of ethanolic and aqueous extracts of Bauhinia variegata Linn. on Gentamicin-Induced Nephrotoxicity in Rats. Ind J Pharm Edu Res. 2011;45(2):192-198.

Dalimartha S. Atlas Tumbuhan Obat Indonesia Jilid I. Ungaran: Trubus Agriwidya, 1999.

Pamungkas DK, Retnaningtyas Y, Wulandari L. Pengujian aktivitas antioksidan kombinasi ekstrak metanol daun mangga gadung (Mangifera indica L. var. gadung) dan ekstrak etanol daun pandan wangi (Pandanus amaryllifolius Roxb.). e-Jurnal Pustaka Kesehatan. 2017;5(1): 46-49.

Prameswari OM, Widjanarko SB. Uji efek ekstrak air daun

pandan wangi terhadap penurunan kadar glukosa darah dan histopatologi tikus diabetes mellitus. Jurnal Pangan dan Agroindustri. 2018;2(2):16-27.

Yulinta NMR, Gelgel KTP, Kardena IM. Efek toksisitas ekstrak daun sirih merah terhadap gambaran mikroskopis ginjal tikus putih Diabetik yang diinduksi aloksan. Buletin Veteriner Udayana. 2013;5(2):114-121.

Kristianingsih I, Wiyono AS. Penggunaan infusa daun alpukat (Persea americana Mill.) dan ekstrak daun pandan (Pandanus amarryllifolius Roxb) sebagai peluruh kalsium batu ginjal secara In Vitro’, Jurna Wiyata.2015;2(1):93-101.

Departemen Kesehatan Republik Indonesia. Farmakope Herbal. Jakarta: Departemen Kesehatan Republik Indonesia, 2010.

Sukmawati, Asmaliani I. Ekstrak etanol daun pandan wangi (Pandanus amaryllifolius Roxb.) sebagai Antinefrotoksisitas berdasarkan penurunan kadar kreatinin tikus. As-Syifaa: Jurnal Farmasi. 2019; 11(2): 93-98.

Dewanti NI, Sofian FF. Review artikel: Aktivitas farmakologi ekstrak daun pandan wangi (Pandanus amaryllifolius Roxb.). Farmaka.2017;15(2):186-193.




DOI: https://doi.org/10.56711/jifa.v13i2.779

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