UJI AKTIVITAS EKSTRAK ETIL ASETAT DAUN KAYU JAWA (Lannea coromandelic (Houtt.) Merr.) TERHADAP PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

Bayu Putra, Safriani Rahman

Abstract


Wound healing is a process of repairing skin and soft tissue when these organs are injured. One of the plants that are presumed to affect wound healing is Kayu Jawa (Lannea coromandelic (Houtt.) Merr.). This research aims to determine the healing effect of burns and the effective dose of ethyl acetate extract of Kayu Jawa’s leaves. The animal test that has been induced using a hot plate with temperature 100oC stuck at the back of mice were divided into 5 (five) groups. Group 1 Na.CMC as Negative control, Group 2 Lanakeloid-E as Positive control, Group III, IV, and V were extract ethyl acetate of Kayu Jawa’s leaves (Lannea coromandelic (Houtt.) Merr) with 3 dosage variations namely, 75 mg/kg, 150 mg/kg, and 300 mg/kg were given topically 2 times a day for 21 days. Then wound area measure using calipers. The group with 300 mg/kg dosage has the best effect for reducing wound area of burns, with statistical value not significantly different from the comparison group (Lanakeloid-E) with a percentage value of 84%.


Keywords


Wound healing, ethyl acetate, Lannea coromandelic (Houtt.) Merr), Rattus norvegicus.

References


Mansjoer A, Wardhani WI, Setiowulan W, Triyanti K, and Savitri R. Kapita Selekta Kedokteran Edisi Tiga. Jakarta. Mediaesculapius, 2000

Sjamsuhidajat R and Dejong W. Buku Ajar: Ilmu Bedah. Jakarta. EGC., 2005.

Baxter C. The Normal Healing Process. In: New Directions in Wound Healing. NJ: E.R. Squlbb & Sons, Inc. Princeton. 1990.

Purnama H, Sriwidodo, Mita SR. Proses Penyembuhan dan Perawatan Luka: Review Sistematik. Farmaka. 2017;15(2): 251-258.

Garg VK, and Paliwal SK. Wound-Healing Activity of Ethanolic And Aqueous Extracts of Ficus benghalensis. J Adv Pharm Technol Res. 2011;2(2):110-114.

Ismail. Luka dan Perawatannya. 2008. http://images.mailmkes.multiplymultiplycontent.com. Diakses tanggal 1 Juni 2019. Pukul 12.30 WITA.

Alam MB, Kwon KR, Lee SH, Lee SH. Lannea coromandelica (Houtt.) Merr. Induces Heme Oxygenase 1 (HO-1) Expression and Reduces Oxidative Stress via the p38/c-Jun N-Terminal Kinase–Nuclear Factor Erythroid 2-Related Factor 2 (p38/JNK–NRF2)-Mediated Antioxidant Pathway. Int J Mol Sci. 2017;18(2): 266.

Rahayu, Sunarti S, Diah P, Suhardjono. Pemanfaatan tanaman obat secara tradisional oleh masyarakat lokal di pulau wawoni. Sulawesi tenggara. Jurnal Biodersivitas. 2006 ;(7)3.

Rahmadani F. Uji Aktivitas Antibakteri Dari Ekstrak Etanol 96% Kulit Batang Kayu Jawa (Lannea coromandelica) Terhadap Bakteri Staphylococcus aureus, Escherichia coli, Helicobacter pylori, Pseudomonas aeruginosa (Skripsi). Jakarta. Fakultas Kedokteran Dan Ilmu Kesehatan. UIN Syarif Hidayatullah. 2015

Saputra A. Uji Aktivitas Antiinflamasi Ekstrak Etanol 96 % Kulit Batang Kayu Jawa (Lannea coromandelica) dengan Metode Stabilitas Membrane Sel Darah Merah Secara In Vitro (Skripsi). Jakarta. Fakultas Kedokteran Dan Ilmu Kesehatan. UIN Syarif Hidayatullah. 2015.

Manik MA, Wahid SMA, Islam A, Pal KT, Ahmed. A Comparative Study of the Antioxidant, Antimicrobial and Thrombolytic Activity of the Bark and Leaves of Lannea coromandelica (Anacardiaceae). International Journal of Pharmaceutical Sciences and Research. 2013;4(7): 2609-2614.

Stalin JD, Babu DT, and Kumar SS. A Study on the Antioxidant and Free Radical Scavenging Property of Lannea Coromandelica Bark Extract. International Journal of Universal Pharmacy and Life Sciences. 2013;3(5), 2249-6793.

Soni H and Singhai AK. A Recent Update of Botanicals for Wound Healing Activity. International Research Journal of Pharmacy. 2012;3(7):1–7.

Ismail I, Paturusi AAE, Aridani I. Aktivitas Antimikroba Hasil Fraksinasi Kortex Kayu Jawa (Lannea coromandelica (Houtt.) Merr.). Biogenesis. 2016;4(2):122-130.

Putra B and Rahman S. Pengaruh Dosis Ekstrak Etanol Daun Kayu Jawa (Lannea coromandelic (Houtt.) Merr.) Terhadap Aktivitas Antiinflamasi Pada Tikus (Rattus norvegicus). As-Syifaa J Farm. 2019;11(2):131-136.

Dirjen POM. Sediaan Galenik. Jakarta Departemen Kesehatan Republik Indonesia., 1986.

Ditjen POM. Farmakope Indonesia Edisi V. Jakarta. Departemen Kesehatan Republik Indonesia. 2004.

Marison MJ. Manajemen Luka. Jakarta.Penerbit Buku Kedokteran EGC. 2004.

Alqahtani SM, Alzahrani MM, Carli A, and Harvey EJ. Burn Management in Orthopaedic Trauma. Journal of bone and joint surgery, incorporated. 2014;2(10): 4

Rahim F, Mimi A, Nurwani PA. Formulasi krim ekstrak etanol daun ubi jalar (Ipomea batatas L.) untuk pengobatan luka bakar. Scientia Jurnal Farmasi dan Kesehatan. 2011;1(1):21-26.

Wahyuningsih S. Soemardji AA and Febiyanti D. Efek Gel Lidah Buaya (Aloe barbadensis Mill) Terhadap Penyembuhan Luka Bakar Eksperimen Pada Tikus Wister Betina. Prosiding seminar nasional tumbuhan obat indonesia XXIX. 2006.

Ambiga SR, Narayanan D, Gowri D, Sukumar D, Madhavan S. Evaluation of Wound Healing Activity ofFlavonoids from Ipommoea carne Jacq. Ancient Science of Life. 2007;26(3):45-51.

Anief M. Formulasi Obat Topikal Dengan Dasar Penyakit Kulit. Yogyakarta. Gajah Mada University Press.1997.

Ajizah A. Sensitivitas Salmonella typhimurium terhadap Ekstrak Daun Psidium Guajava L. Bioscientie. 2004.1 (1): 31-38.




DOI: https://doi.org/10.33096/jifa.v12i2.720

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