UJI AKTIVITAS ANTIOKSIDAN ISOLAT FUNGI ENDOFIT DAUN GALING-GALING (Cayratia trifolia L.) DENGAN METODE 1,1-DIPHENYL-2-PICRYLHYDRAZIL (DPPH)

Magfirah Abdullah, Fitriana Fitriana, St. Maryam

Abstract


Endophytic fungi generally produce secondary metabolites that have beneficial biological activities such as anticancer, antiviral, antibacterial or antioxidant compounds. The aim of this study was to examine the antioxidant activity of galling-galing leaf endophytic fungi isolate (Cayratia trifolia L.) by 1,1-diphenyl-2-picrylhydrazil (DPPH) method. This study used IDGG-03 endophytic fungi isolate fermented for 21 days using MYB medium then extracted and evaporation was carried out. The fermentate extract obtained was then tested qualitatively and quantitatively for antioxidant activity. Qualitative results using TLC plates indicate that galling-leaf fungi isolates have antioxidant activity which is characterized by the presence of yellow spots on a purple background. And in the quantitative test IC50 68,133 µg / mL was obtained, which indicated the antioxidant activity of galling-vane leaf endophytic fungi isolate (Cayratia trifolia L.) classified as a strong antioxidant.


Keywords


Antioxidants, Cayratia trifolia L., endophytic fungi, 1,1-diphenyl-2-picrylhydrazil (DPPH)

References


Hasiani VV, Ahmad I, Rijai L. Isolasi Jamur Endofit dan Produksi Metabolit Sekunder Antioksidan dari Daun Pacar (Lawsonia inermis L.). Jurnal Sains dan Kesehatan. 2015;1(4): 146-153.

Prihatiningtias W, Wahyuningsih, MSH. Prospek Mikroba Endofit Sebagai Sumber Senyawa Bioaktif. Jogjakarta. Fakultas Farmasi Universitas Gadjah Mada, 2006.

Radji M. Peranan Bioteknologi dan Mikroba Endofit dalam Pengembangan Obat Herbal. Majalah Ilmu Kefarmasian. 2005;2(3).

Kuncahyono I and Sunardi. Uji Aktivitas Antiokisdan Ekstrak Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap 1,1-Diphenyl-2-Picrylhidrazyl (DPPH). Seminar Nasional Teknologi. 2007:1-9.

Elliott T. Mikrobiologi Kedokteran & Infeksi. Jakarta. EGC, 2013.

Kjer J, Debbab A, Aly AH, Proksch P. Methods for isolation of marine-dereved endophytic fungi and their bioactive secondary products. Nat Protoc. 2010;5(3);479-90

Noverita. Identifikasi Kapang dan Khamir Penyebab Penyakit Manusia pada Sumber Air Minum Penduduk pada Sungai Ciliwung dan Sumber Air Sekitarnya. Vis Vitalis. 2009;2(2):15-19.

Cholisoh Z and Utami W. Aktivitas penangkap Radikal Ekstrak Etanol 70% Biji Jengkol (Archidendron jiringa). PHARMACON. 2008;9(1):33-40.

Kumala S. Antimicrobial Activity of Secondary Metabolites Produced by Endophytic Fungi Isolated from Stems of Jati Tree (Tectona grandis L.F). International Journal of Pharmacetiucal Science and Research. 2015;6(6):2349-2353.

Strobel GA. Natural Products from Endophytic Microorganism. Journal Natural Product, 2004.

Saha A, Mandal P, Dasgupta S, Saha D. Influence of Culture Media and Environmental Factors an Mycelia Growth and Sporulation of Lasiopdiplodia the obromae (Pat.) Griffon and Maubl. Journal of Enviromental Biology. 2008;29(3):407-410.

Fitriana, Maryam St, Naid T, Maryana. Penelusuran Fungi Endofit Sebagai Penghasil Senyawa Antibiotika dari Daun Nanas (Ananas comosus (L) Merr). Jurnal Farmasi Asy-Syifaa. 2016;8(01):01-08

Rendowaty A, Djamaan A and Handayani D. Waktu Kultivasi Optimal dan Aktivitas Antibakteri dari Ekstrak Etil Asetat Jamur Simbion Aspergillus unguis (WR8) dengan Haliclona fascigera. Jurnal Sains Farmasi. 2017;4(2):49-54.

Sarker SD, Nahar L. Chemistry for Pharmacy Student (General, Organic and Natural Product Chemistry). Chichester: J Wiley, 2007.

Arora, Daljit S. Assay of Antioxidant Potential of Two Aspergillus Isolates by Different Methods Under Various Physio-Chemical Conditions. Brazilian Journal of Microbiology. 2009;41:765-777.

Srujana M, Lakhmidevi N. Antioxidant Studies of Methanolic Extract and Active Fraction Obtained From Ichnocarpus frutescens. Antioxidant determinations by the use of a stable free radical. International Journal of Pharma and Bio Science. 2017;8(1):110-117.

Sayuti K and Yenrina R. Antioksidan Alami dan Sintetik. Padang. Andalas University Press, 2015.

Handayani S, Najib A and Wati NP. Uji Aktivitas Antioksidan Ekstrak Daun Daruju (Acanthus ilicifolius L.) Dengan Metode Peredamna Radikal Bebas 1,1-Diphenyil-2-Picrylhidrazil (DPPH). Jurnal Fitofarmaka Indonesia. 2018;5(2):299-308.

Winarsi H. Antioksidan Alami dan Radikal Bebas. Yogyakarta: Kanisius., 2011.




DOI: https://doi.org/10.33096/jifa.v12i2.617

Refbacks

  • There are currently no refbacks.