AKTIVITAS ANTIBAKTERI FRAKSI-FRAKSI DAUN Colocacia esculenta L. TERHADAP Salmonella thypi Dan Staphylococcus aureus SECARA BIOAUTOGRAPHY-TLC
Abstract
Antibacterial activity test has been done on Colocacia esculenta L leaves fractions. The aim of the research was to determine the antibacterial activity of Colocacia esculenta L leaves fractions delivered from Pare-Pare District of South Sulawesi on Salmonella thypii and Staphyllococcus aureus by means of vacuum liquid chromatography and Bioautographic-TLC. Results of fractionations of the ethanol extract by the former method using eluent of 100 mL n-hexane, n-hexane:ethyl acetate (10:1; 1:1), 100 mL ethyl acetate, ethyl acetate:methanol (10:1; 1:1) and 100 mL methanol were obtained 7 fractions. Bioautographic-TLC assay then carried out on the obtained fractions they were fraction 3,4,5,6 and 7 respectively and obtained Rf value 0.80 was possessed antibacterial activity on both tested bacteria.
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DOI: https://doi.org/10.56711/jifa.v8i2.224
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ISSN: 2085-4714 | e-ISSN: 2502-9444
Editor's Address:
Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia
E-mail: jurnal.farmasi@umi.ac.id