AKTIVITAS ANTIBAKTERI ISOLAT FUNGI ENDOFIT BIJI LABU KUNING (Cucurbita moschata ex. Poir)

Siska Nuryanti, Fitriana Fitriana, Fitriah Anggraini Ohorella

Abstract


Cucurbita moschata ex. Poir are one of the medicinal plants that have many benefits, including antidiabetic, antifungal, antibacterial, anti-inflammatory, and antioxidant effects. The content of compounds in yellow pumpkin seeds, such as phenols, alkaloids, and triterpenoids, This study aims to isolate endophytic fungi from yellow pumpkin seeds and test the antibacterial activity of isolates of yellow pumpkin bii endophytic fungi using the Agar diffusion method. The results of the isolation of yellow pumpkin seed endophytic fungi obtained 3 isolates, followed by macroscopic examination and fermentation of the 3 isolates with Maltose Yeast Broth (MYB) media and testing antibacterial activity by Agar diffusion method. Based on the results of the study, 3 isolates with the codes IJBL1, IJBL2, and IJBL3 were obtained that have antibacterial potential. The highest inhibitory zone was produced by IJBL3 endophytic fungi isolate with an inhibitory zone diameter of 12.39 mm against Staphylococcus aureus bacteria

Keywords


Agar diffusion; Antibacterial; Cucurbita moschata ex. Poir; Endophytic Fungi

References


Taechowisan T, Lu C, Shen Y, Lumayong S. 4-Arylcoumarin From Endophytic Streptomices Aureofaciens CMUAc130 and Their Antifungal Activityle. Journal Annals of Microbiology. 2005; 44:63–66

Ramos SAF et al. Endophytic Microorganisms from Bauhinia monandra Leaves: Isolation, Antimicrobial Activities and Interaction with Galactose-Specific Lectin BmoLL. Afr J Microbiol Res. 2016; 10(17):600–607

Strobel GA. Endophytes as Sources of Bioactive Products. Microbes Infect. 2003; 5(6):535–544

Alvin A, Miller KI, Neilan BA. Exploring the Potential of Endophytes from Medicinal Plants as Sources of Antimycobacterial Compounds. Microbiol Res. 2014; 169(7–8):483–495

Kuncoro H, Mulawarman U. Mini Review Jamur Endofit, Biodiversitas, Potensi, Dan Prospek Penggunaannya Sebagai Sumber Bahan Obat Baru. 2016; (January 2011):247–262

Latief A. Obat Tradisional. Jakarta: EGC. 2013

Tasmiatun S, Rustina. Uji Aktivitas Antioksidan dan Antibakteri Ekstrak Etil Asetat Biji Labu Kuning (Cucurbita moschata Duch. Poir). 2016

Nuryanti S et al. Antibacterial Activity of Endophytic Fungi Isolated from Portulaca oleracea L. AIP Conf Proc.; 2595(1). DOI: 10.1063/5.0124016/2890693

Mitra A, Herwin H, Nuryanti S. Isolation of Endophytic Fungi From Patchouli Leaves (Pogostemon cablin Benth) as Antibacterial Against Pathogenic Bacteria by Bioautography and Agar Diffusion. Journal Microbiology Science. 2023; 3(1):45–56

Nuryanti S, Rusli, Astuti R. Potensi Fungi Endofit Biji Pinang Sebagai Antibakteri Terhadap Escherichia coli dan Salmonella thypi. Green Medical Journal. 2019; 1(1):87–96

Radji M. Peranan Bioteknologi Dan Mikroba Endofit Dalam Pengembangan Obat Herbal. Majalah Ilmu Kefarmasian. 2005; 2(3):113–126

Davis WW, Stout TR. Disc Plate Method of Microbiological Antibiotic Assay. II. Novel Procedure Offering Improved Accuracy. Appl Microbiol. 1971; 22(4):666–670




DOI: https://doi.org/10.56711/jifa.v15i2.1069

Refbacks

  • There are currently no refbacks.


Indexed by:

     

ISSN: 2085-4714 | e-ISSN: 2502-9444

Editor's Address:

Faculty of Pharmacy, Univeristas Muslim Indonesia
2nd Campus of UMI: Jl. Urip Sumoharjo km. 5 , Makassar, South Sulawesi, Indonesia

E-mail: jurnal.farmasi@umi.ac.id

View My Stats