UJI FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN EKSTRAK METANOL DAUN ZODIA (Evodia suaveolens)

Khoirul Ngibad, Lilla Puji Lestari

Sari


Zodia (Evodia suaveolens) plant is commonly used by the community to repel mosquitoes. The plant is native to Papua but has been widely cultivated in Java such as: Batu, Depok, Bogor, Bandung and Surabaya. The aim of this study is to determine the antioxidant activity of zodia leaves methanol extract. In addition, to determine the class of secondary metabolites of the methanol extract of zodia leaves. The extraction process was carried out by maceration method using methanol solvent for 3 days then the filtrate was concentrated using a rotary evaporator. The antioxidant potential of zodia leaves methanol extract was tested using the DPPH method (1,1-Diphenyl-2-picrylhydrazyl) with a UV-Vis spectrophotometer while the determination of secondary metabolite compounds from zodia leaves methanol extract was carried out qualitatively using reagents. The results of the phytochemical test showed that the methanol extract of zodia plant leaves contained group of alkaloid, flavonoids, and tannins compounds while the antioxidant activity test of zodia methanol extract using the DPPH method were shown with an IC50 value of 55,69 ppm while These results indicated that the zodia leaves methanol extracts have strong category of antioxidant activity.


Kata Kunci


In vitro antioxidants; DPPH; methanol extract of Evodia suaveolens; fitochemical test

Teks Lengkap:

Fulltext PDF

Referensi


Zhou Yunfenga, Li Lin, Sun Lan, Zhou Lidong XY. In comparison with vitamin C and butylated hydroxytoluene, the antioxidant capacity of aqueous extracts from buds and flowers of Lonicera japonica Thunb. J Tradit Chinese Med. 2019;38(3):373–9.

Karuna DS, Dey P, Das S, Kundu A, Bhakta T. Journal of Traditional and Complementary Medicine In vitro antioxidant activities of root extract of Asparagus racemosus. J Tradit Chinese Med Sci. 2018;8(1):60–5.

Samkeliso Takaidza, Fanyana Mtunzi MP. Analysis of the phytochemical contents and antioxidant activities of crude extracts from Tulbaghia species. J Tradit Chinese Med. 2018;38(2):272–9.

Ade Ferdinan ABP. Uji Aktivitas Antioksidan dari Ekstrak Jantung Pisang Kepok (Musa paradisiaca L.) Pontianak. J Ilm Ibnu Sina. 2018;3(1):88–96.

Reksi Sundu, Sapri HN. Uji Fitokimia dan Aktivitas Antioksidan Ekstrak Etanol Umbi Paku Atai Merah (Angiopteris ferox COPEL). J Ilm Ibnu Sina. 2018;3(1):97–105.

Hidayati MD, Ersam T, Shimizu K, Fatmawati S. Antioxidant activity of Syzygium polyanthum extracts. Indones J Chem. 2017;17(1):49–53.

Ngibad K. Efektivitas Kombinasi Ekstrak Etanol Daun Bunga Matahari dan Tanaman Anting-Anting sebagai Antimalaria Secara In Vivo. J Farm Galen. (5(1):12–9.

Ngibad K. Kandungan Senyawa Kimia dan Bioaktivitas dari Selaginella doederleinii Hieron. 2018

Anwar K, Triyasmono L. Kandungan total fenolik, total flavonoid, dan aktivitas antioksidan ekstrak etanol buah mengkudu (Morinda citrifolia L.). J Pharmascience. 2019;3(1):83–92.

Febriyenti F, Suharti N, Lucida H, Husni E, Sedona O. Karakterisasi dan Studi Aktivitas Antioksidan dari Ekstrak Etanol Secang (Caesalpinia sappan L.). J Sains Farm Klin. 2018;5(1):23–7.

Haiyul Fadhli, Ainun Nurain Nurdin MO. Potensi Antioksidan dari Ekstrak Kulit Batang Bauhinia semibifida Roxb. J Ilm Ibnu Sina. 2019;4(1):77–87.

Isrianto PL. Bisnis Usaha Perbanyakan Tanaman Zodia ( Evodia suaveolens ) Sebagai Tanaman Pengusir Nyamuk di Kota Surabaya. Inovasi. 2016;18(2):102–9.

Basundari SA, Tarwotjo U, Kusdiyantini E. Pengaruh Kandungan Ekstrak Daun Zodia (Evodia suaveolens) terhadap Mortalitas Larva Nyamuk Aedes aegypti. Bioma Berk Ilm Biol. 2018;20(1):51–8.

Lestari MS, Himawan T, Abadi AL, Retnowati R. Toxicity and phytochemistry test of methanol extract of several plants from Papua using Brine Shrimp Lethality Test (BSLT). J Chem Pharm Res. 2015;7(4):866–72.

Maryuni AE. Isolasi dan Identifikasi Senyawa Antibakteri Minyak Atsiri Daun Zodia (Evodia sp.). 2008.

Brewer MS. Natural antioxidants: sources, compounds, mechanisms of action, and potential applications. Compr Rev food Sci food Saf. 2011;10(4):221–47.

Porkony J, Yanishlieva N, Gordon M. Introduction of Antioxidant. Antioxidants in food: Practical applications. 2001. 1–3 p.

Ngibad K. Phytochemical Screening of Sunflower Leaf (Helianthus annuus) and Anting-Anting (Acalypha indica Linn) Plant Ethanol Extract. Borneo J Pharm. 2019;2(1):24–30.

Adebiyi OE, Olayemi FO, Ning-Hua T, Guang-Zhi Z. In vitro antioxidant activity, total phenolic and flavonoid contents of ethanol extract of stem and leaf of Grewia carpinifolia. Beni-Suef Univ J Basic Appl Sci [Internet]. 2017;6(1):10–4. Available from: http://linkinghub.elsevier.com/retrieve/pii/S2314853516301007

Lestari M., Himawan T, Abadi A., Retnowati R. Toxicity and phytochemistry test of methanol extract of several plants from papua using Brine Shrimp Lethality Test (BSLT). J Chem Pharm Res. 2015;7(4):866–72.

Harborne JB. Metode fitokimia: Penuntun cara modern menganalisis tumbuhan. Bandung Penerbit ITB. 1987;78.

Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochem Biophys. 2009;53(2):75–100.

Fathurrachman DA. Pengaruh konsentrasi pelarut terhadap aktivitas antioksidan ekstrak etanol daun sirsak (Annona muricata Linn) dengan metode peredaman radikal bebas DPPH. 2014;

Malangngi L, Sangi M, Paendong J. Penentuan kandungan tanin dan uji aktivitas antioksidan ekstrak biji buah alpukat (Persea americana Mill.). J MIPA. 2012;1(1):5–10.

Dungir SG, Katja DG, Kamu VS. Aktivitas antioksidan ekstrak fenolik dari kulit buah manggis (Garcinia mangostana L.). J MIPA. 2012;1(1):11–5.

Sami FJ, Soekamto NH, Firdaus F, Latip J. Uji Aktivitas Antioksidan Beberapa Ekstrak Alga Coklat Sargassum polycystum dan Turbinaria Deccurens Asal Pulau Dutungan Sulawesi Selatan Terhadap Radikal DPPH. J Kim Ris. 2019;4(1):1–6.


Refbacks

  • Saat ini tidak ada refbacks.


View My Stats