UJI AKTIVITAS EKSTRAK ETANOL KULIT PISANG MAS (Musa acuminata (AA Goup)) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus Musculus) YANG DIINDUKSI ALOKSAN

Kadek Evi D.P. Dewi, Abdul Wahid Jamaluddin, Fedri Rell

Sari


Diabetes mellitus (DM) is a metabolic diseases caused by impaired insulin secretion. The prevalence of DM in pets increased from 2006 until 2015.Therapy by using insulin feared can be worse for patient, so yhat alternative medicine is needed like banana peel. The aims of this study isto determine the effect ofethanol extract of Mas banana peel (Musa acuminata (AA Group)) to decrease level of glucose in mice’s blood (Mus musculus) were induced by alloxan. This research was conducted in June 2017 at Biopharmaceutical Laboratory Faculty of Pharmacy, Hasanuddin University. This study uses 24 male mice which divided into 6 groups where the normal control (standard diet), negative control (Na.Cmc 1%), positive control (Metformin 140mg/KgBB) and treatment group by ethanol extract of Mas banana peel 1%, 5%, and 25%. The treatment was given for 10 days. Data were analyzed using one way Anova. The result of this study showed that ethanol extract in banana peel decrease the level of blood glucose which the decrease percentage are 43,15%, 54,61%, 74,28%. The summary of this research is the treatment by using variant concentration of ethanol extract of Mas banana peelfor 10 days give the significant effect for decreasing the level of blood glucose (p<0,05) and the activity of ethanol extract of Mas Banana Peel 25% are not give the significant effect with metformin dose 140mg/KgBB (p>0,05).


Kata Kunci


Alloxan, blood glucose, hyperglychemia, mas banana peel, mice.

Teks Lengkap:

Fulltext PDF

Referensi


Murray RK, Granner DK, Rodwell VW. Biokimia harper (27 ed.) Jakarta : Penerbit Buku Kedokteran EGC, 2009.

Chaiyasut C, Kusirisin W, Lailerd N, Lertrakarnoon P, Suttajit M, Srichairatanakool S. Effect of Phenolic Compounds of Fermented Thai Indigenous Plants on Oxidative Stress in Strepzotosin-Induced Diabetic Rats. Research Article.2011;15(2):118123.

Bruyette D. Diabetes Melllitus [internet] 2016. [Diakses pada 4 Desember 2017]. Tersedia di : http://www.merckvetmanual.com/endocrine-system/the-pancreas/diabetes-mellitus

American Veterinary Medical Association. Diabetic Pets. USA [internet]. 2010. [Diakses pada 13 Oktober 2017] Tersedia di :https://www.avma.org/public/PetCare/Pages/Diabetes-inPets.aspx.

Jamaluddin AW, Muslimin LW, Djide MN. Ekstrak Daun Jambu Biji (Psidium guajava L) sebagai inhibitor pertumbuhan bakteri zoonosis proteus mirabilis yang diisolasi dari daging ayam broiler. Jurnal As-Syifaa.2018;10(01):30-36.

Someya S, Yoshiki Y, Okubo K. Antioxidant Compounds From Banana (Musa cavendish). Journal Food Chemistry.2002;79(3):351-4.

Kanazawa K, Saksakibara H. High Content of Dopamine, A Strong Antioxidant in Cavendish Banana.J Agric Food Chem. 2000;25(3):844-48.

Sighal M, Ratra P. Antioxidant Activity, Total Flavonoid and Total Phenolic Content of Musa acuminata Peel Exctracts.Global J. Pharmacol.2013;7(2):188-22.

Satriany P. Pengaruh Pemberian Ekstrak Herba Daun Sendok (Plantago major L.) Terhadap Kadar Glukosa Darah Mencit Balb/c Induksi Streptozotocin (Skripsi). Surakarta: Universitas Sebelas Maret,2010.

Departemen Kesehatan RI. Acuan Sediaan Herbal. Direktorat Pengawas Obat dan Makanan. Jakarta: Departemen Kesehatan Republik Indonesia,2000.

Harborne JB. Metode Fitokimia : Penuntun Cara Modern Menganalisis Tumbuhan, terjemahan Kosasih Padmawinata dan Iwang Soediro. Bandung: Institut Teknologi Bandung,1987.

Kusumawati D. Bersahabat Dengan Hewan Coba. Yogyakarta: Gadjah Mada University Press,2004.

Pasaribu R, Hutahaean S, Ilyas S. Uji Antihiperglikemika Ekstrak Etanol Daun Kembang Bulan (Tithonia diversifolia) pada Mencit (Mus musculus) yang diinduksi Diabetes Dengan Aloksan. J Biosains,2015;1(2)2460-6804.

Suharmiati. Pengujian Bioaktivitas Anti Diabetes Mellitus Tumbuhan Obat.Badan Penelitian dan Pengembangan Pelayanan dan Teknologi Kesehatan. Surabaya: Departemen Kesehatan Republik Indonesia,2003.

Adams, Kathrynn A, Lawrence, Eva K. Research Methods Statistics, and Applications. California: Sage Publications Inc.,2015.

Gembong T. Morfologi Tumbuhan. Yogyakarta: Gadjah Mada University Press,2007.

Rosdiana, Nurul F. Uji Efek Antihiperglikemik Ekstrak Etil Asetat Lumut Hati (Mastigophora diclados) Dengan Metode Induksi Aloksan (Skripsi). Jakarta: Universitas Islam Negeri Syarif Hidayatullah,2013.

Syamsuni HA. Ilmu Resep. Jakarta: Penerbit Buku Kedokteran EGC,2006.

Gritter RJ, Bobbit JM, A. E. Schwarting. Bandung: Pengantar Kromatografi. Institut Teknologi Bandung,1991.

Ashley C, et al. Pro-Poor Tourism Strategies: Making Tourism Work for the Poor, A Review of Experience [internet] 2001. [Diakses pada 9 Februari 2017]. Tersedia di : http://www.propoortourism.org.uk/ppt_report.pdf.

Lenzen S. The Mechanism of Alloxan and Streptozotocin Induced Diabetes. Diabetologia 2008 51:216–226 DOI 10.1007/s00125-007-0886-7

Szkudelski T. The Mechanishm of Alloxan and streotozotocin Action in β Cells of the Rat pankreas. Physiology research.2001;50:536-54.

Robinson T. Kandungan Organik Tumbuhan Tinggi. Edisi ke 4. Terjemahan Koasasih Padmawinata. Bandung: ITB Press,1995.

Prakash A. Antioksidant Activity. Journal Medallions Laboratories. 2000;19(2):1-4.

Aprilia, Fitri I, Salim MN, Daud R, Armansyah T, Asmila N, Jamin F. Pengaruh pemberian Kacang Pnajang (Vigna unguiculata) Terhadap Kadar Glukosa Darah Mencit (Mus musculus) yang Diinduksi Aloksan. J Medika Veterinaria. 2015;9(2):0853-1943.

Kaneto et al. Benefical effect of Antioxidants in Diabetic Possible Protection of Pancreatic B-Cells Against Glucose Toxicity[internet] 1999. [Diakses pada 15 Agustus 2017]. Tersedia di : http://diabetes.diabetesjournals.org/cgi/reprint/48/12/2398.pdf.[online].

Winarsi H. Isoflavon : Berbagai Sumber, Sifat, Dan Manfaatnya pada penyakit Degenerative. Yogyakarta: Gadjah Mada university Press,2006.

Puspati NKS. Anthara MS., Dharmayudha AAGO. Pertambahan Bobot Bada Tikus Diabetes Dengan Pemberian Ekstrak Etanol Buah Naga Daging Putih. Journal Indoneisa Medicus Veterinus.2013;2(2):224-34.

Harapan, Jamil KF, Hayati Z, Muhammad I. Peran Puasa Dalam Remodelling Sel Enteroendokrin Untuk Mencegah Diabetes Mellitus Tipe 2. JIMKI.2010;1(1): 36-40.

Rao A. Gurfinkel. The Bioactivity of Saponins Triterpenoid and Steroidal Glycosides. Drugs Metab Grug Interact.2000 211-35.

Kumari M, Jain S. Tannins : An Antinutrient with Positive Effect to Manage Diabetes. Research Journal of Recent Science.2012;1(12) : 70 -1.

Yokuzawa T, Cho EJ, Park CH, Kim HJ. Rivies Article Protective Effect of Proanthocyanidin Agaisnst Diabetic Oxidative Stress.Evidence- Based Complementary and Alternative Medicine.2012;22:204

Fidrianny I, Padmawinata K, Soetarno S, Yulinah E. Efek Antihipertensi dan Hipotensi beberapa Fraksi dari Ekstrak Etanol Umbi Lapis Kucai (Allium schoenoprasum L., Lliliaceae). Jurnal Matematika dan Sains.2003; 8(4):147-150.

Widowati W. Potensi Antioksidan sebagai Antidiabetes. JKM.2008; 7(2):1-10.

Dor. Adult Pancreatic ß are Performed by Cell Duplication Rather than Stem Cell Differentiation. Nature.2005:429.

Chougale AD, Shirmant NP, Pradeep MG, Akalpita UA. Optimization of Alloxan Dose in Essential to Induce Stable Diabetes for Prolonged Period. Assian Journal of Biochemistry. 2007;2(6):402-408.

Doughari JH. Antimicrobial Activity of Tamarindus indica Linn. Tropical Journal of Pharmaceutical Research. 2006;5(2):597-60.


Refbacks

  • Saat ini tidak ada refbacks.


View My Stats