UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN ARBENAN (Duchesnea indica (Jacks.) Focke) DENGAN METODE DPPH

Nuraziza Nuraziza, Seniwati Dali, Risda Waris

Sari


Arbenan (Duchesnea indica (Jacks.) Focke) is a plant belonging to Rosaceae family which server as an antioxidant, flavonoids. This research aimed to determine the antioxidant activity of ethanol extract of arbenan leaves using DPPH. The leaves were extracted using maceration method with a sample size of 300 gs dissolved using ethanol 70%, then obtained the viscous extract of 32,49 gs. The arrest of free radicals for each tested sample under the inhibition of DPPH. The tests were conducted at the 4 concentration series of 10, 20, 30, and 40 ppm by using methanol p.a by the addition of 0,5 mL with 3,5 mL DPPH of 30 ppm. Antioxidant activity absorbance was measured by a spectrophotometer at the wavelength of   515 nm and IC50 value was calculated based on the absorbance data. The calculations showed that the Arbenan leaves have a very strong antioxidant activity with IC50 value of 30,20 µg/mL.


Kata Kunci


Antioxidant, Ethanol extract of arbenan leaves [Dunchesnea indica (Jacks) Focke], DPPH.

Teks Lengkap:

Fulltext PDF

Referensi


Chaudury RR. Herbal Medicine for Human Health. , New Delhi : World Health Organization; 1992.

Hutapea JR. Inventaris Tanaman Obat Indonesia. Balai Penerbitan dan Pengembangan Kesehatan; 1991.

Dalimartha S. Atlas Tumbuhan Obat Indonesia, Jilid 3. Jakarta: Puspaswara; 2004.

Artanti N, Jamilah dan Hartati S. Laporan Teknis Sub Tolok Ukur Pengembangan Senyawa Potensial antikanker dari Taxus sumatrana dan Benalu. Serpong : Puslit Kimia LIPI; 2003.

Harborne JB. Metode Fitokimia 5 Penuntun cara modern menganalisa tumbuhan. Terbitan Kedua. Terjemahan Kosasih Padmawinata dan Iwang Soediro. Bandung: ITB; 1987.

Arief S. Radikal Bebas, Laporan Penelitian, Ilmu Kesehatan Anak, Surabaya: Fakultas Kedokteran UNAIR; 2008.

Wijeratne SSK, Cuppett SL, Schlegel V. Hydrogen Peroxide Induced Oxidative Stress Damage and Antioxidant Enzyme Response in Caco-Human colon cells. Journal Agricultural and Food Chemistry 2005;53:8768-8774.

Deng, Wangyuan, Guangzhong. A novel antioxidant activity index (AAU) for natural products using the DPPH assay. Food Chemistry 2011;125:1430–1435.

Tiwari, Kumar, Kaur Mandeep, Kaur Gurpreet & Kaur Harleem. Phytochemical Screening and Extraction: A Review. Internationale Pharmaceutica Sciencia 2011;1(1).

Brand-Williams W, Cuvelier ME and Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensmitted-Wissenschaft und-Technologie/Food Science and Technology; 1995.

Ahmad AR, Mun’im A and Elya B. Study of antioxidant activity With reduction of free radikal DPPH and Xanthine Oxidase inhibitor of the extract Ruella tuberosa linn lear. Internasional Research joernal of pharmacy 2012;102:29 47,48.

Pratiwi D, Wahdaningsih S, Isnindar. Uji Aktivitas Antioksidan Daun Bawang Mekah (Eleutherine Americana Merr.) dengan Metode DPPH. Trad. Med. Journal 2013;18(1).

Ukieyanna E. Aktivitas Antioksidan, Kadar Fenolik, dan Flavonoid Total Tumbuhan Suruhan (Peperomia pellucid L. Kunth). Bogor : Fakultas Teknologi Pertanian Institut Pertanian Bogor; 2012.

Molyneux P. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin. J Sci Technol 2004;26(2):212.

Andayani R, Yovita L, Maimunah. Penentuan aktivitas antioksidan, kadar fenolat total dan likopen pada buah Tomat (Solanum lycopersicum l). J Sains dan Teknologi Farmasi 2008;13(1): 31-37.

Nic´iforovic´ N, et al. Antioxidant Activity of Selected Plant Species; Potential New Sources of Natural Antioxidants, Food and Chemical Toxicology 2010;48:3125–3130.

Ridho EA. Uji Aktivitas Antioksidan Ekstrak Metanol Buah Lakum (Cayratia trifolia) Dengan Metode Dpph (2,2-Difenil-1-Pikrilhidrazil). Pontianak: Fakultas Kedokteran Universitas Tanjungpura; 2013.

Es-Safi NE, S. Ghidouche PH. Ducrot, Molecule 12 (2007) 2228.


Refbacks

  • Saat ini tidak ada refbacks.


View My Stats